Experiment: Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_02635 and IAI47_02640 overlap by 27 nucleotides IAI47_02640 and IAI47_02645 are separated by 56 nucleotides
IAI47_02635: IAI47_02635 - isovaleryl-CoA dehydrogenase, at 546,378 to 548,006
_02635
IAI47_02640: IAI47_02640 - biofilm peroxide resistance protein BsmA, at 547,980 to 548,306
_02640
IAI47_02645: IAI47_02645 - Cache 3/Cache 2 fusion domain-containing protein, at 548,363 to 550,294
_02645
Position (kb) 547
548
549 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 547.011 kb on + strand, within IAI47_02635 at 547.012 kb on - strand, within IAI47_02635 at 547.028 kb on + strand, within IAI47_02635 at 547.028 kb on + strand, within IAI47_02635 at 547.029 kb on - strand, within IAI47_02635 at 547.029 kb on - strand, within IAI47_02635 at 547.093 kb on + strand, within IAI47_02635 at 547.094 kb on - strand, within IAI47_02635 at 547.094 kb on - strand, within IAI47_02635 at 547.094 kb on - strand, within IAI47_02635 at 547.094 kb on - strand, within IAI47_02635 at 547.166 kb on + strand, within IAI47_02635 at 547.166 kb on + strand, within IAI47_02635 at 547.166 kb on + strand, within IAI47_02635 at 547.166 kb on + strand, within IAI47_02635 at 547.166 kb on + strand, within IAI47_02635 at 547.166 kb on + strand, within IAI47_02635 at 547.167 kb on - strand, within IAI47_02635 at 547.167 kb on - strand, within IAI47_02635 at 547.167 kb on - strand, within IAI47_02635 at 547.366 kb on + strand, within IAI47_02635 at 547.366 kb on + strand, within IAI47_02635 at 547.366 kb on + strand, within IAI47_02635 at 547.367 kb on - strand, within IAI47_02635 at 547.367 kb on - strand, within IAI47_02635 at 547.367 kb on - strand, within IAI47_02635 at 547.367 kb on - strand, within IAI47_02635 at 547.537 kb on - strand, within IAI47_02635 at 547.537 kb on - strand, within IAI47_02635 at 547.632 kb on + strand, within IAI47_02635 at 547.652 kb on + strand, within IAI47_02635 at 547.652 kb on + strand, within IAI47_02635 at 547.652 kb on + strand, within IAI47_02635 at 547.652 kb on + strand, within IAI47_02635 at 547.653 kb on - strand, within IAI47_02635 at 547.653 kb on - strand, within IAI47_02635 at 547.653 kb on - strand, within IAI47_02635 at 547.653 kb on - strand, within IAI47_02635 at 547.735 kb on + strand, within IAI47_02635 at 547.736 kb on - strand, within IAI47_02635 at 547.736 kb on - strand, within IAI47_02635 at 547.736 kb on - strand, within IAI47_02635 at 547.767 kb on - strand, within IAI47_02635 at 547.812 kb on - strand, within IAI47_02635 at 547.882 kb on + strand at 547.882 kb on + strand at 547.882 kb on + strand at 547.883 kb on - strand at 547.883 kb on - strand at 547.883 kb on - strand at 547.883 kb on - strand at 547.890 kb on + strand at 547.891 kb on - strand at 547.891 kb on - strand at 547.891 kb on - strand at 547.891 kb on - strand at 547.971 kb on - strand at 548.014 kb on + strand, within IAI47_02640 at 548.033 kb on - strand, within IAI47_02640 at 548.077 kb on + strand, within IAI47_02640 at 548.078 kb on - strand, within IAI47_02640 at 548.078 kb on - strand, within IAI47_02640 at 548.080 kb on + strand, within IAI47_02640 at 548.081 kb on - strand, within IAI47_02640 at 548.081 kb on - strand, within IAI47_02640 at 548.081 kb on - strand, within IAI47_02640 at 548.081 kb on - strand, within IAI47_02640 at 548.081 kb on - strand, within IAI47_02640 at 548.081 kb on - strand, within IAI47_02640 at 548.145 kb on + strand, within IAI47_02640 at 548.145 kb on + strand, within IAI47_02640 at 548.145 kb on + strand, within IAI47_02640 at 548.145 kb on + strand, within IAI47_02640 at 548.146 kb on - strand, within IAI47_02640 at 548.146 kb on - strand, within IAI47_02640 at 548.146 kb on - strand, within IAI47_02640 at 548.156 kb on + strand, within IAI47_02640 at 548.156 kb on + strand, within IAI47_02640 at 548.156 kb on + strand, within IAI47_02640 at 548.157 kb on - strand, within IAI47_02640 at 548.157 kb on - strand, within IAI47_02640 at 548.160 kb on - strand, within IAI47_02640 at 548.160 kb on - strand, within IAI47_02640 at 548.160 kb on - strand, within IAI47_02640 at 548.160 kb on - strand, within IAI47_02640 at 548.160 kb on - strand, within IAI47_02640 at 548.160 kb on - strand, within IAI47_02640 at 548.160 kb on - strand, within IAI47_02640 at 548.289 kb on + strand at 548.289 kb on + strand at 548.289 kb on + strand at 548.290 kb on - strand at 548.290 kb on - strand at 548.290 kb on - strand at 548.290 kb on - strand at 548.290 kb on - strand at 548.290 kb on - strand at 548.293 kb on + strand at 548.293 kb on + strand at 548.293 kb on + strand at 548.294 kb on - strand at 548.307 kb on - strand at 548.307 kb on - strand at 548.341 kb on + strand at 548.341 kb on + strand at 548.341 kb on + strand at 548.341 kb on + strand at 548.341 kb on + strand at 548.341 kb on + strand at 548.342 kb on - strand at 548.342 kb on - strand at 548.342 kb on - strand at 548.342 kb on - strand at 548.342 kb on - strand at 548.342 kb on - strand at 548.342 kb on - strand at 548.381 kb on + strand at 548.382 kb on - strand at 548.413 kb on + strand at 548.414 kb on - strand at 548.486 kb on - strand at 548.500 kb on + strand at 548.500 kb on + strand at 548.500 kb on + strand at 548.500 kb on + strand at 548.501 kb on - strand at 548.501 kb on - strand at 548.503 kb on + strand at 548.503 kb on + strand at 548.503 kb on + strand at 548.504 kb on - strand at 548.504 kb on - strand at 548.504 kb on - strand at 548.504 kb on - strand at 548.504 kb on - strand at 548.516 kb on - strand at 548.516 kb on - strand at 548.516 kb on - strand at 548.516 kb on - strand at 548.516 kb on - strand at 548.650 kb on + strand, within IAI47_02645 at 548.650 kb on + strand, within IAI47_02645 at 548.651 kb on - strand, within IAI47_02645 at 548.651 kb on - strand, within IAI47_02645 at 548.727 kb on - strand, within IAI47_02645 at 548.833 kb on + strand, within IAI47_02645 at 548.860 kb on + strand, within IAI47_02645 at 548.861 kb on - strand, within IAI47_02645 at 548.875 kb on + strand, within IAI47_02645 at 548.875 kb on + strand, within IAI47_02645 at 548.875 kb on + strand, within IAI47_02645 at 548.875 kb on + strand, within IAI47_02645 at 548.875 kb on + strand, within IAI47_02645 at 548.876 kb on - strand, within IAI47_02645 at 548.876 kb on - strand, within IAI47_02645 at 548.876 kb on - strand, within IAI47_02645 at 548.876 kb on - strand, within IAI47_02645 at 548.876 kb on - strand, within IAI47_02645 at 548.876 kb on - strand, within IAI47_02645 at 548.918 kb on - strand, within IAI47_02645 at 548.918 kb on - strand, within IAI47_02645 at 548.918 kb on - strand, within IAI47_02645 at 549.055 kb on + strand, within IAI47_02645 at 549.055 kb on + strand, within IAI47_02645 at 549.055 kb on + strand, within IAI47_02645 at 549.056 kb on - strand, within IAI47_02645 at 549.056 kb on - strand, within IAI47_02645 at 549.196 kb on + strand, within IAI47_02645 at 549.203 kb on - strand, within IAI47_02645 at 549.228 kb on + strand, within IAI47_02645 at 549.228 kb on + strand, within IAI47_02645 at 549.228 kb on + strand, within IAI47_02645 at 549.228 kb on + strand, within IAI47_02645 at 549.228 kb on + strand, within IAI47_02645 at 549.229 kb on - strand, within IAI47_02645 at 549.229 kb on - strand, within IAI47_02645 at 549.229 kb on - strand, within IAI47_02645 at 549.285 kb on + strand, within IAI47_02645 at 549.285 kb on + strand, within IAI47_02645 at 549.286 kb on - strand, within IAI47_02645 at 549.286 kb on - strand, within IAI47_02645
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 547,011 + IAI47_02635 0.39 +2.2 547,012 - IAI47_02635 0.39 -0.1 547,028 + IAI47_02635 0.40 -2.1 547,028 + IAI47_02635 0.40 -0.6 547,029 - IAI47_02635 0.40 -0.1 547,029 - IAI47_02635 0.40 +1.7 547,093 + IAI47_02635 0.44 +1.5 547,094 - IAI47_02635 0.44 -0.7 547,094 - IAI47_02635 0.44 +2.4 547,094 - IAI47_02635 0.44 -1.2 547,094 - IAI47_02635 0.44 -2.0 547,166 + IAI47_02635 0.48 -1.3 547,166 + IAI47_02635 0.48 +1.5 547,166 + IAI47_02635 0.48 -1.1 547,166 + IAI47_02635 0.48 +3.4 547,166 + IAI47_02635 0.48 -0.7 547,166 + IAI47_02635 0.48 +1.9 547,167 - IAI47_02635 0.48 -0.6 547,167 - IAI47_02635 0.48 -0.8 547,167 - IAI47_02635 0.48 +1.0 547,366 + IAI47_02635 0.61 -0.7 547,366 + IAI47_02635 0.61 -0.3 547,366 + IAI47_02635 0.61 +0.7 547,367 - IAI47_02635 0.61 -0.5 547,367 - IAI47_02635 0.61 +2.0 547,367 - IAI47_02635 0.61 +1.3 547,367 - IAI47_02635 0.61 +0.2 547,537 - IAI47_02635 0.71 -0.5 547,537 - IAI47_02635 0.71 -1.4 547,632 + IAI47_02635 0.77 -1.1 547,652 + IAI47_02635 0.78 +0.6 547,652 + IAI47_02635 0.78 +1.2 547,652 + IAI47_02635 0.78 -0.3 547,652 + IAI47_02635 0.78 +1.0 547,653 - IAI47_02635 0.78 -0.9 547,653 - IAI47_02635 0.78 +0.1 547,653 - IAI47_02635 0.78 -1.2 547,653 - IAI47_02635 0.78 +0.1 547,735 + IAI47_02635 0.83 -0.3 547,736 - IAI47_02635 0.83 -0.5 547,736 - IAI47_02635 0.83 +0.3 547,736 - IAI47_02635 0.83 -1.1 547,767 - IAI47_02635 0.85 -0.7 547,812 - IAI47_02635 0.88 -1.2 547,882 + -0.1 547,882 + +0.9 547,882 + -0.1 547,883 - -0.4 547,883 - +0.8 547,883 - -0.9 547,883 - +0.1 547,890 + +1.4 547,891 - -0.9 547,891 - +0.1 547,891 - -0.3 547,891 - -0.0 547,971 - -2.1 548,014 + IAI47_02640 0.10 +0.5 548,033 - IAI47_02640 0.16 -1.5 548,077 + IAI47_02640 0.30 -1.0 548,078 - IAI47_02640 0.30 +1.0 548,078 - IAI47_02640 0.30 +1.1 548,080 + IAI47_02640 0.31 -1.2 548,081 - IAI47_02640 0.31 -1.9 548,081 - IAI47_02640 0.31 -2.3 548,081 - IAI47_02640 0.31 -0.4 548,081 - IAI47_02640 0.31 -0.5 548,081 - IAI47_02640 0.31 +1.0 548,081 - IAI47_02640 0.31 +1.4 548,145 + IAI47_02640 0.50 -1.7 548,145 + IAI47_02640 0.50 +0.7 548,145 + IAI47_02640 0.50 -0.3 548,145 + IAI47_02640 0.50 +2.1 548,146 - IAI47_02640 0.51 +0.8 548,146 - IAI47_02640 0.51 +0.3 548,146 - IAI47_02640 0.51 -1.0 548,156 + IAI47_02640 0.54 -0.7 548,156 + IAI47_02640 0.54 -0.5 548,156 + IAI47_02640 0.54 +1.7 548,157 - IAI47_02640 0.54 -1.4 548,157 - IAI47_02640 0.54 +4.0 548,160 - IAI47_02640 0.55 -2.2 548,160 - IAI47_02640 0.55 +0.2 548,160 - IAI47_02640 0.55 -0.2 548,160 - IAI47_02640 0.55 -0.8 548,160 - IAI47_02640 0.55 +0.9 548,160 - IAI47_02640 0.55 -0.5 548,160 - IAI47_02640 0.55 +0.8 548,289 + -1.3 548,289 + -1.2 548,289 + -0.6 548,290 - +1.9 548,290 - +4.0 548,290 - +1.6 548,290 - +0.4 548,290 - +0.2 548,290 - +0.6 548,293 + +1.0 548,293 + -1.0 548,293 + -0.5 548,294 - +0.9 548,307 - +2.8 548,307 - +0.1 548,341 + -0.7 548,341 + -0.8 548,341 + -1.2 548,341 + -1.2 548,341 + +0.2 548,341 + -1.2 548,342 - +1.7 548,342 - +1.7 548,342 - +3.1 548,342 - -1.2 548,342 - -0.6 548,342 - -0.5 548,342 - -0.1 548,381 + -0.4 548,382 - +1.3 548,413 + -1.1 548,414 - -0.4 548,486 - +0.6 548,500 + -0.6 548,500 + +0.5 548,500 + -2.5 548,500 + -0.2 548,501 - +0.6 548,501 - -0.6 548,503 + -0.2 548,503 + -0.3 548,503 + -1.2 548,504 - +1.6 548,504 - +1.4 548,504 - -0.5 548,504 - +0.3 548,504 - +0.3 548,516 - -1.3 548,516 - -1.1 548,516 - +3.0 548,516 - +0.6 548,516 - +1.0 548,650 + IAI47_02645 0.15 -0.4 548,650 + IAI47_02645 0.15 -1.7 548,651 - IAI47_02645 0.15 +1.4 548,651 - IAI47_02645 0.15 -1.1 548,727 - IAI47_02645 0.19 -1.0 548,833 + IAI47_02645 0.24 -1.0 548,860 + IAI47_02645 0.26 -0.4 548,861 - IAI47_02645 0.26 -2.5 548,875 + IAI47_02645 0.27 -0.8 548,875 + IAI47_02645 0.27 -1.5 548,875 + IAI47_02645 0.27 -2.2 548,875 + IAI47_02645 0.27 +0.8 548,875 + IAI47_02645 0.27 +0.1 548,876 - IAI47_02645 0.27 +2.8 548,876 - IAI47_02645 0.27 +2.9 548,876 - IAI47_02645 0.27 +0.2 548,876 - IAI47_02645 0.27 +0.2 548,876 - IAI47_02645 0.27 +0.8 548,876 - IAI47_02645 0.27 +0.8 548,918 - IAI47_02645 0.29 -1.4 548,918 - IAI47_02645 0.29 +0.5 548,918 - IAI47_02645 0.29 +0.3 549,055 + IAI47_02645 0.36 +1.9 549,055 + IAI47_02645 0.36 +1.0 549,055 + IAI47_02645 0.36 +0.0 549,056 - IAI47_02645 0.36 +1.4 549,056 - IAI47_02645 0.36 +1.5 549,196 + IAI47_02645 0.43 +0.2 549,203 - IAI47_02645 0.43 -0.9 549,228 + IAI47_02645 0.45 -1.0 549,228 + IAI47_02645 0.45 +0.3 549,228 + IAI47_02645 0.45 -0.2 549,228 + IAI47_02645 0.45 -0.1 549,228 + IAI47_02645 0.45 -0.4 549,229 - IAI47_02645 0.45 -1.4 549,229 - IAI47_02645 0.45 +0.4 549,229 - IAI47_02645 0.45 -0.5 549,285 + IAI47_02645 0.48 -0.8 549,285 + IAI47_02645 0.48 -1.1 549,286 - IAI47_02645 0.48 +1.0 549,286 - IAI47_02645 0.48 +3.8
Or see this region's nucleotide sequence