Experiment: Varel_Bryant medium with Sulfaquinoxaline 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2035 and BT2036 are separated by 87 nucleotides BT2036 and BT2037 are separated by 298 nucleotides BT2037 and BT2038 are separated by 86 nucleotides
BT2035: BT2035 - putative ABC transporter ATP-binding protein (NCBI ptt file), at 2,554,287 to 2,555,924
BT2035
BT2036: BT2036 - conserved hypothetical protein (NCBI ptt file), at 2,556,012 to 2,556,593
BT2036
BT2037: BT2037 - hypothetical protein (NCBI ptt file), at 2,556,892 to 2,557,302
BT2037
BT2038: BT2038 - cation efflux system protein (NCBI ptt file), at 2,557,389 to 2,558,660
BT2038
Position (kb)
2556
2557 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2555.201 kb on - strand, within BT2035 at 2555.403 kb on - strand, within BT2035 at 2555.603 kb on + strand, within BT2035 at 2555.640 kb on - strand, within BT2035 at 2555.823 kb on + strand at 2555.823 kb on + strand at 2555.922 kb on + strand at 2555.952 kb on + strand at 2555.968 kb on + strand at 2555.968 kb on + strand at 2555.987 kb on + strand at 2556.038 kb on + strand at 2556.040 kb on + strand at 2556.079 kb on - strand, within BT2036 at 2556.204 kb on - strand, within BT2036 at 2556.310 kb on + strand, within BT2036 at 2556.361 kb on + strand, within BT2036 at 2556.466 kb on + strand, within BT2036 at 2556.466 kb on + strand, within BT2036 at 2556.466 kb on + strand, within BT2036 at 2556.479 kb on - strand, within BT2036 at 2556.549 kb on + strand at 2556.794 kb on - strand at 2556.831 kb on - strand at 2556.832 kb on + strand at 2556.848 kb on + strand at 2556.879 kb on - strand at 2556.882 kb on + strand at 2556.882 kb on + strand at 2556.917 kb on + strand at 2556.917 kb on + strand at 2556.918 kb on - strand at 2556.926 kb on + strand at 2556.927 kb on - strand at 2556.934 kb on - strand, within BT2037 at 2556.945 kb on + strand, within BT2037 at 2556.946 kb on - strand, within BT2037 at 2556.963 kb on + strand, within BT2037 at 2557.219 kb on + strand, within BT2037 at 2557.261 kb on - strand, within BT2037 at 2557.339 kb on + strand at 2557.371 kb on - strand at 2557.389 kb on - strand at 2557.401 kb on - strand at 2557.455 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Sulfaquinoxaline 0.02 mM remove 2,555,201 - BT2035 0.56 -1.3 2,555,403 - BT2035 0.68 +0.6 2,555,603 + BT2035 0.80 -1.2 2,555,640 - BT2035 0.83 -1.8 2,555,823 + -0.5 2,555,823 + +1.6 2,555,922 + -1.3 2,555,952 + -1.6 2,555,968 + -1.0 2,555,968 + +0.0 2,555,987 + -1.0 2,556,038 + -1.6 2,556,040 + -1.5 2,556,079 - BT2036 0.12 -2.2 2,556,204 - BT2036 0.33 -1.4 2,556,310 + BT2036 0.51 -3.6 2,556,361 + BT2036 0.60 -2.4 2,556,466 + BT2036 0.78 -0.6 2,556,466 + BT2036 0.78 -3.1 2,556,466 + BT2036 0.78 -1.6 2,556,479 - BT2036 0.80 -2.5 2,556,549 + -3.1 2,556,794 - +0.4 2,556,831 - -2.0 2,556,832 + +1.8 2,556,848 + +0.0 2,556,879 - +2.0 2,556,882 + +0.0 2,556,882 + -1.2 2,556,917 + -0.3 2,556,917 + -0.6 2,556,918 - -1.1 2,556,926 + -0.8 2,556,927 - -0.6 2,556,934 - BT2037 0.10 -0.7 2,556,945 + BT2037 0.13 -0.2 2,556,946 - BT2037 0.13 +0.2 2,556,963 + BT2037 0.17 -0.7 2,557,219 + BT2037 0.80 -0.3 2,557,261 - BT2037 0.90 +0.6 2,557,339 + -1.3 2,557,371 - +1.6 2,557,389 - +1.3 2,557,401 - -0.2 2,557,455 - +0.0
Or see this region's nucleotide sequence