Experiment: Pyruvate-Sulfate (60-15mM) with Selenate 750uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Gly and tRNA-Cys are separated by 11 nucleotides tRNA-Cys and tRNA-Gly are separated by 7 nucleotides tRNA-Gly and tRNA-Gly are separated by 32 nucleotides tRNA-Gly and DVU2151 are separated by 93 nucleotides DVU2151 and DVU2152 are separated by 9 nucleotides
DVU_tRNA-Gly-1: tRNA-Gly - tRNA with anticodon GCC for Gly (VIMSS-AUTO), at 2,250,385 to 2,250,459
tRNA-Gly
DVU_tRNA-Cys-1: tRNA-Cys - tRNA with anticodon GCA for Cys (VIMSS-AUTO), at 2,250,471 to 2,250,545
tRNA-Cys
DVU_tRNA-Gly-2: tRNA-Gly - tRNA with anticodon GCC for Gly (VIMSS-AUTO), at 2,250,553 to 2,250,627
tRNA-Gly
DVU_tRNA-Gly-3: tRNA-Gly - tRNA with anticodon CCC for Gly (VIMSS-AUTO), at 2,250,660 to 2,250,734
tRNA-Gly
DVU2151: DVU2151 - conserved hypothetical protein (TIGR), at 2,250,828 to 2,251,187
DVU2151
DVU2152: DVU2152 - hypothetical protein (TIGR), at 2,251,197 to 2,252,213
DVU2152
Position (kb)
2250
2251
2252 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2249.863 kb on + strand at 2249.871 kb on - strand at 2249.871 kb on - strand at 2249.898 kb on + strand at 2249.903 kb on + strand at 2250.167 kb on + strand at 2250.188 kb on + strand at 2250.234 kb on - strand at 2250.834 kb on - strand at 2250.843 kb on - strand at 2250.863 kb on + strand at 2250.865 kb on + strand, within DVU2151 at 2250.873 kb on - strand, within DVU2151 at 2250.873 kb on - strand, within DVU2151 at 2250.892 kb on + strand, within DVU2151 at 2251.016 kb on + strand, within DVU2151 at 2251.035 kb on - strand, within DVU2151 at 2251.063 kb on - strand, within DVU2151 at 2251.081 kb on - strand, within DVU2151 at 2251.088 kb on + strand, within DVU2151 at 2251.088 kb on + strand, within DVU2151 at 2251.096 kb on - strand, within DVU2151 at 2251.106 kb on - strand, within DVU2151 at 2251.106 kb on - strand, within DVU2151 at 2251.136 kb on - strand, within DVU2151 at 2251.160 kb on + strand at 2251.192 kb on - strand at 2251.482 kb on + strand, within DVU2152 at 2251.539 kb on + strand, within DVU2152 at 2251.648 kb on - strand, within DVU2152 at 2251.668 kb on - strand, within DVU2152 at 2251.675 kb on + strand, within DVU2152 at 2251.683 kb on - strand, within DVU2152 at 2251.838 kb on + strand, within DVU2152 at 2251.838 kb on + strand, within DVU2152 at 2251.846 kb on - strand, within DVU2152 at 2251.852 kb on + strand, within DVU2152 at 2251.886 kb on - strand, within DVU2152 at 2251.901 kb on + strand, within DVU2152 at 2251.903 kb on + strand, within DVU2152 at 2251.909 kb on - strand, within DVU2152 at 2252.106 kb on + strand, within DVU2152 at 2252.176 kb on + strand at 2252.181 kb on + strand at 2252.181 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Sulfate (60-15mM) with Selenate 750uM remove 2,249,863 + +0.2 2,249,871 - -0.6 2,249,871 - +0.8 2,249,898 + -1.5 2,249,903 + -0.0 2,250,167 + -0.4 2,250,188 + -2.6 2,250,234 - -0.4 2,250,834 - -0.2 2,250,843 - +1.3 2,250,863 + -0.4 2,250,865 + DVU2151 0.10 +0.8 2,250,873 - DVU2151 0.12 -0.3 2,250,873 - DVU2151 0.12 -0.1 2,250,892 + DVU2151 0.18 -1.3 2,251,016 + DVU2151 0.52 +0.6 2,251,035 - DVU2151 0.57 -0.1 2,251,063 - DVU2151 0.65 +0.4 2,251,081 - DVU2151 0.70 +1.2 2,251,088 + DVU2151 0.72 +2.0 2,251,088 + DVU2151 0.72 +1.6 2,251,096 - DVU2151 0.74 +0.9 2,251,106 - DVU2151 0.77 -0.6 2,251,106 - DVU2151 0.77 +1.0 2,251,136 - DVU2151 0.86 -0.5 2,251,160 + -0.4 2,251,192 - -0.6 2,251,482 + DVU2152 0.28 +1.9 2,251,539 + DVU2152 0.34 -1.5 2,251,648 - DVU2152 0.44 +0.2 2,251,668 - DVU2152 0.46 -1.2 2,251,675 + DVU2152 0.47 -0.6 2,251,683 - DVU2152 0.48 -0.1 2,251,838 + DVU2152 0.63 +0.6 2,251,838 + DVU2152 0.63 -0.4 2,251,846 - DVU2152 0.64 -0.1 2,251,852 + DVU2152 0.64 -1.9 2,251,886 - DVU2152 0.68 -0.1 2,251,901 + DVU2152 0.69 -0.6 2,251,903 + DVU2152 0.69 -0.7 2,251,909 - DVU2152 0.70 -0.2 2,252,106 + DVU2152 0.89 +0.1 2,252,176 + +0.1 2,252,181 + +0.2 2,252,181 + +1.2
Or see this region's nucleotide sequence