Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_410 and Pf6N2E2_411 are separated by 76 nucleotides Pf6N2E2_411 and Pf6N2E2_412 are separated by 183 nucleotides Pf6N2E2_412 and Pf6N2E2_413 are separated by 32 nucleotides Pf6N2E2_413 and Pf6N2E2_414 are separated by 2 nucleotides Pf6N2E2_414 and Pf6N2E2_415 are separated by 17 nucleotides
Pf6N2E2_410 - hypothetical protein, at 480,118 to 480,660
_410
Pf6N2E2_411 - Nitrogen regulation protein NR(I), at 480,737 to 480,892
_411
Pf6N2E2_412 - hypothetical protein, at 481,076 to 481,336
_412
Pf6N2E2_413 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 481,369 to 482,442
_413
Pf6N2E2_414 - Chemotaxis protein CheD, at 482,445 to 482,921
_414
Pf6N2E2_415 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 482,939 to 483,775
_415
Position (kb) 481
482
483 Strain fitness (log2 ratio) -1
0
1
2 at 480.719 kb on - strand at 481.061 kb on - strand at 481.354 kb on + strand at 481.355 kb on - strand at 481.355 kb on - strand at 481.664 kb on + strand, within Pf6N2E2_413 at 481.664 kb on + strand, within Pf6N2E2_413 at 482.422 kb on + strand at 482.422 kb on + strand at 482.422 kb on + strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.442 kb on + strand at 482.461 kb on - strand at 482.461 kb on - strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.481 kb on + strand at 482.482 kb on - strand at 482.482 kb on - strand at 482.482 kb on - strand at 482.482 kb on - strand at 482.482 kb on - strand at 482.740 kb on + strand, within Pf6N2E2_414 at 482.741 kb on - strand, within Pf6N2E2_414 at 482.741 kb on - strand, within Pf6N2E2_414 at 482.741 kb on - strand, within Pf6N2E2_414 at 482.743 kb on - strand, within Pf6N2E2_414 at 482.743 kb on - strand, within Pf6N2E2_414 at 482.909 kb on - strand at 482.909 kb on - strand at 482.909 kb on - strand at 483.025 kb on - strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.091 kb on - strand, within Pf6N2E2_415 at 483.091 kb on - strand, within Pf6N2E2_415 at 483.091 kb on - strand, within Pf6N2E2_415 at 483.240 kb on + strand, within Pf6N2E2_415 at 483.241 kb on - strand, within Pf6N2E2_415 at 483.315 kb on - strand, within Pf6N2E2_415 at 483.315 kb on - strand, within Pf6N2E2_415 at 483.393 kb on + strand, within Pf6N2E2_415 at 483.394 kb on - strand, within Pf6N2E2_415
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 480,719 - -1.3 481,061 - -0.8 481,354 + +0.1 481,355 - +0.3 481,355 - -0.4 481,664 + Pf6N2E2_413 0.27 -1.6 481,664 + Pf6N2E2_413 0.27 -0.2 482,422 + -0.3 482,422 + +1.8 482,422 + -0.3 482,423 - +0.4 482,423 - +0.3 482,423 - -0.1 482,423 - +0.7 482,423 - +1.4 482,423 - +0.7 482,423 - -0.4 482,423 - +0.0 482,442 + -0.6 482,461 - +0.5 482,461 - +0.0 482,462 + +0.8 482,462 + +0.6 482,462 + -0.2 482,462 + -1.3 482,462 + -0.1 482,462 + +0.5 482,462 + -0.3 482,463 - +0.7 482,463 - -0.8 482,463 - +0.4 482,463 - +0.5 482,463 - -0.2 482,463 - -1.2 482,463 - +0.1 482,463 - +0.5 482,481 + -0.2 482,482 - -0.1 482,482 - -0.2 482,482 - +0.6 482,482 - -0.6 482,482 - -0.5 482,740 + Pf6N2E2_414 0.62 +0.7 482,741 - Pf6N2E2_414 0.62 -0.1 482,741 - Pf6N2E2_414 0.62 -0.9 482,741 - Pf6N2E2_414 0.62 +0.3 482,743 - Pf6N2E2_414 0.62 +0.2 482,743 - Pf6N2E2_414 0.62 -0.4 482,909 - +0.1 482,909 - -0.3 482,909 - +0.2 483,025 - Pf6N2E2_415 0.10 -0.1 483,090 + Pf6N2E2_415 0.18 +0.0 483,090 + Pf6N2E2_415 0.18 +1.1 483,090 + Pf6N2E2_415 0.18 +0.4 483,090 + Pf6N2E2_415 0.18 +0.5 483,091 - Pf6N2E2_415 0.18 +0.3 483,091 - Pf6N2E2_415 0.18 -1.5 483,091 - Pf6N2E2_415 0.18 +0.1 483,240 + Pf6N2E2_415 0.36 -0.3 483,241 - Pf6N2E2_415 0.36 -1.3 483,315 - Pf6N2E2_415 0.45 +0.5 483,315 - Pf6N2E2_415 0.45 -0.6 483,393 + Pf6N2E2_415 0.54 +0.5 483,394 - Pf6N2E2_415 0.54 +0.3
Or see this region's nucleotide sequence