Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3715 and Pf6N2E2_3716 are separated by 146 nucleotides Pf6N2E2_3716 and Pf6N2E2_3717 overlap by 4 nucleotides Pf6N2E2_3717 and Pf6N2E2_3718 overlap by 14 nucleotides
Pf6N2E2_3715 - FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase / (3R)-hydroxymyristoyl-[ACP] dehydratase (EC 4.2.1.-), at 4,327,782 to 4,329,461
_3715
Pf6N2E2_3716 - FIG143263: Glycosyl transferase, at 4,329,608 to 4,330,342
_3716
Pf6N2E2_3717 - Lysophospholipid acyltransferase, at 4,330,339 to 4,331,283
_3717
Pf6N2E2_3718 - Putative histidine ammonia-lyase protein, at 4,331,270 to 4,332,808
_3718
Position (kb)
4330
4331
4332 Strain fitness (log2 ratio)
-2
-1
0
1 at 4329.390 kb on + strand at 4329.391 kb on - strand at 4329.391 kb on - strand at 4329.439 kb on + strand at 4329.439 kb on + strand at 4329.440 kb on - strand at 4329.440 kb on - strand at 4329.440 kb on - strand at 4329.440 kb on - strand at 4329.459 kb on + strand at 4329.460 kb on - strand at 4329.528 kb on + strand at 4329.528 kb on + strand at 4329.529 kb on - strand at 4329.639 kb on - strand at 4329.639 kb on - strand at 4329.966 kb on - strand, within Pf6N2E2_3716 at 4329.996 kb on - strand, within Pf6N2E2_3716 at 4329.996 kb on - strand, within Pf6N2E2_3716 at 4330.038 kb on + strand, within Pf6N2E2_3716 at 4330.038 kb on + strand, within Pf6N2E2_3716 at 4330.039 kb on - strand, within Pf6N2E2_3716 at 4330.434 kb on - strand, within Pf6N2E2_3717 at 4330.477 kb on + strand, within Pf6N2E2_3717 at 4330.477 kb on + strand, within Pf6N2E2_3717 at 4330.477 kb on + strand, within Pf6N2E2_3717 at 4330.478 kb on - strand, within Pf6N2E2_3717 at 4330.478 kb on - strand, within Pf6N2E2_3717 at 4330.478 kb on - strand, within Pf6N2E2_3717 at 4330.478 kb on - strand, within Pf6N2E2_3717 at 4330.478 kb on - strand, within Pf6N2E2_3717 at 4330.478 kb on - strand, within Pf6N2E2_3717 at 4330.478 kb on - strand, within Pf6N2E2_3717 at 4330.522 kb on + strand, within Pf6N2E2_3717 at 4330.522 kb on + strand, within Pf6N2E2_3717 at 4331.209 kb on + strand at 4331.209 kb on + strand at 4331.209 kb on + strand at 4331.209 kb on + strand at 4331.209 kb on + strand at 4331.210 kb on - strand at 4331.210 kb on - strand at 4331.440 kb on + strand, within Pf6N2E2_3718 at 4331.441 kb on - strand, within Pf6N2E2_3718 at 4331.441 kb on - strand, within Pf6N2E2_3718 at 4331.445 kb on - strand, within Pf6N2E2_3718 at 4331.526 kb on - strand, within Pf6N2E2_3718 at 4331.526 kb on - strand, within Pf6N2E2_3718 at 4331.527 kb on + strand, within Pf6N2E2_3718 at 4331.528 kb on - strand, within Pf6N2E2_3718 at 4331.528 kb on - strand, within Pf6N2E2_3718 at 4331.528 kb on - strand, within Pf6N2E2_3718 at 4331.528 kb on - strand, within Pf6N2E2_3718 at 4331.528 kb on - strand, within Pf6N2E2_3718 at 4331.528 kb on - strand, within Pf6N2E2_3718 at 4331.738 kb on + strand, within Pf6N2E2_3718 at 4331.738 kb on + strand, within Pf6N2E2_3718 at 4331.738 kb on + strand, within Pf6N2E2_3718 at 4331.739 kb on - strand, within Pf6N2E2_3718 at 4331.739 kb on - strand, within Pf6N2E2_3718 at 4331.739 kb on - strand, within Pf6N2E2_3718 at 4331.925 kb on - strand, within Pf6N2E2_3718 at 4332.243 kb on + strand, within Pf6N2E2_3718 at 4332.244 kb on - strand, within Pf6N2E2_3718 at 4332.264 kb on - strand, within Pf6N2E2_3718 at 4332.274 kb on + strand, within Pf6N2E2_3718
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 4,329,390 + -1.4 4,329,391 - -0.7 4,329,391 - -1.2 4,329,439 + -0.3 4,329,439 + -0.4 4,329,440 - -1.4 4,329,440 - -1.1 4,329,440 - +0.6 4,329,440 - -0.7 4,329,459 + -0.7 4,329,460 - -0.8 4,329,528 + -0.4 4,329,528 + -0.4 4,329,529 - -0.1 4,329,639 - -0.1 4,329,639 - -0.9 4,329,966 - Pf6N2E2_3716 0.49 -2.0 4,329,996 - Pf6N2E2_3716 0.53 +0.2 4,329,996 - Pf6N2E2_3716 0.53 -1.6 4,330,038 + Pf6N2E2_3716 0.59 -0.2 4,330,038 + Pf6N2E2_3716 0.59 -0.7 4,330,039 - Pf6N2E2_3716 0.59 -0.4 4,330,434 - Pf6N2E2_3717 0.10 +0.8 4,330,477 + Pf6N2E2_3717 0.15 -2.0 4,330,477 + Pf6N2E2_3717 0.15 -0.9 4,330,477 + Pf6N2E2_3717 0.15 -1.1 4,330,478 - Pf6N2E2_3717 0.15 -0.8 4,330,478 - Pf6N2E2_3717 0.15 -0.5 4,330,478 - Pf6N2E2_3717 0.15 -1.6 4,330,478 - Pf6N2E2_3717 0.15 +0.0 4,330,478 - Pf6N2E2_3717 0.15 -0.7 4,330,478 - Pf6N2E2_3717 0.15 -2.0 4,330,478 - Pf6N2E2_3717 0.15 +0.0 4,330,522 + Pf6N2E2_3717 0.19 -0.5 4,330,522 + Pf6N2E2_3717 0.19 -1.4 4,331,209 + -0.3 4,331,209 + -1.4 4,331,209 + -0.5 4,331,209 + -1.2 4,331,209 + +0.5 4,331,210 - -0.5 4,331,210 - -0.1 4,331,440 + Pf6N2E2_3718 0.11 -0.1 4,331,441 - Pf6N2E2_3718 0.11 -2.4 4,331,441 - Pf6N2E2_3718 0.11 -0.5 4,331,445 - Pf6N2E2_3718 0.11 -0.2 4,331,526 - Pf6N2E2_3718 0.17 -0.5 4,331,526 - Pf6N2E2_3718 0.17 +0.4 4,331,527 + Pf6N2E2_3718 0.17 -0.7 4,331,528 - Pf6N2E2_3718 0.17 +1.6 4,331,528 - Pf6N2E2_3718 0.17 -0.3 4,331,528 - Pf6N2E2_3718 0.17 -0.1 4,331,528 - Pf6N2E2_3718 0.17 -1.2 4,331,528 - Pf6N2E2_3718 0.17 +0.4 4,331,528 - Pf6N2E2_3718 0.17 +0.5 4,331,738 + Pf6N2E2_3718 0.30 +0.7 4,331,738 + Pf6N2E2_3718 0.30 +0.4 4,331,738 + Pf6N2E2_3718 0.30 +0.3 4,331,739 - Pf6N2E2_3718 0.30 -1.0 4,331,739 - Pf6N2E2_3718 0.30 -0.0 4,331,739 - Pf6N2E2_3718 0.30 -0.2 4,331,925 - Pf6N2E2_3718 0.43 -2.4 4,332,243 + Pf6N2E2_3718 0.63 -1.0 4,332,244 - Pf6N2E2_3718 0.63 -1.2 4,332,264 - Pf6N2E2_3718 0.65 -1.1 4,332,274 + Pf6N2E2_3718 0.65 -0.0
Or see this region's nucleotide sequence