Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_368

Experiment: Calcium chloride 0.0125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_367 and Pf6N2E2_368 are separated by 254 nucleotidesPf6N2E2_368 and Pf6N2E2_369 are separated by 40 nucleotides Pf6N2E2_367 - Acetylornithine deacetylase (EC 3.5.1.16), at 433,628 to 434,785 _367 Pf6N2E2_368 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 435,040 to 436,497 _368 Pf6N2E2_369 - Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83), at 436,538 to 437,617 _369 Position (kb) 435 436 437Strain fitness (log2 ratio) -2 -1 0 1at 434.318 kb on + strand, within Pf6N2E2_367at 434.528 kb on + strand, within Pf6N2E2_367at 434.783 kb on + strandat 434.792 kb on + strandat 434.792 kb on + strandat 434.792 kb on + strandat 434.792 kb on + strandat 434.792 kb on + strandat 434.792 kb on + strandat 434.793 kb on - strandat 434.793 kb on - strandat 434.793 kb on - strandat 434.793 kb on - strandat 434.793 kb on - strandat 434.800 kb on + strandat 434.800 kb on + strandat 434.800 kb on + strandat 434.801 kb on - strandat 434.801 kb on - strandat 434.801 kb on - strandat 434.893 kb on + strandat 434.893 kb on + strandat 434.893 kb on + strandat 434.894 kb on - strandat 434.894 kb on - strandat 434.894 kb on - strandat 434.894 kb on - strandat 434.894 kb on - strandat 434.894 kb on - strandat 435.007 kb on + strandat 435.018 kb on + strandat 435.088 kb on + strandat 435.089 kb on - strandat 435.174 kb on - strandat 435.427 kb on + strand, within Pf6N2E2_368at 435.952 kb on + strand, within Pf6N2E2_368at 435.952 kb on + strand, within Pf6N2E2_368at 435.952 kb on + strand, within Pf6N2E2_368at 435.953 kb on - strand, within Pf6N2E2_368at 436.236 kb on + strand, within Pf6N2E2_368at 436.239 kb on + strand, within Pf6N2E2_368at 436.295 kb on - strand, within Pf6N2E2_368at 436.297 kb on + strand, within Pf6N2E2_368at 436.297 kb on + strand, within Pf6N2E2_368at 436.444 kb on + strandat 436.445 kb on - strandat 436.445 kb on - strandat 436.445 kb on - strandat 436.459 kb on + strandat 436.686 kb on - strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.688 kb on + strand, within Pf6N2E2_369at 436.689 kb on - strand, within Pf6N2E2_369at 436.754 kb on + strand, within Pf6N2E2_369at 436.844 kb on + strand, within Pf6N2E2_369at 436.844 kb on + strand, within Pf6N2E2_369at 436.845 kb on - strand, within Pf6N2E2_369at 436.955 kb on + strand, within Pf6N2E2_369at 437.405 kb on + strand, within Pf6N2E2_369at 437.405 kb on + strand, within Pf6N2E2_369at 437.405 kb on + strand, within Pf6N2E2_369at 437.405 kb on + strand, within Pf6N2E2_369at 437.406 kb on - strand, within Pf6N2E2_369at 437.490 kb on - strand, within Pf6N2E2_369

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Per-strain Table

Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M
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434,318 + Pf6N2E2_367 0.60 -0.0
434,528 + Pf6N2E2_367 0.78 +0.8
434,783 + +0.5
434,792 + +0.3
434,792 + -0.5
434,792 + +0.4
434,792 + +0.0
434,792 + -0.6
434,792 + +0.2
434,793 - +1.3
434,793 - +0.8
434,793 - +1.0
434,793 - -0.1
434,793 - -0.4
434,800 + +0.2
434,800 + -0.4
434,800 + +0.2
434,801 - -0.4
434,801 - -1.0
434,801 - +0.5
434,893 + -0.0
434,893 + +0.2
434,893 + -1.0
434,894 - +0.8
434,894 - +0.7
434,894 - +0.8
434,894 - -0.6
434,894 - +0.3
434,894 - +1.2
435,007 + +0.6
435,018 + +0.9
435,088 + +0.1
435,089 - -0.3
435,174 - -0.5
435,427 + Pf6N2E2_368 0.27 +0.1
435,952 + Pf6N2E2_368 0.63 -0.0
435,952 + Pf6N2E2_368 0.63 -2.3
435,952 + Pf6N2E2_368 0.63 -1.7
435,953 - Pf6N2E2_368 0.63 -0.1
436,236 + Pf6N2E2_368 0.82 -0.4
436,239 + Pf6N2E2_368 0.82 -0.5
436,295 - Pf6N2E2_368 0.86 -0.4
436,297 + Pf6N2E2_368 0.86 -0.2
436,297 + Pf6N2E2_368 0.86 +0.8
436,444 + -0.4
436,445 - +1.1
436,445 - +0.4
436,445 - -1.2
436,459 + +0.2
436,686 - Pf6N2E2_369 0.14 +0.3
436,688 + Pf6N2E2_369 0.14 +0.1
436,688 + Pf6N2E2_369 0.14 -0.1
436,688 + Pf6N2E2_369 0.14 -0.1
436,688 + Pf6N2E2_369 0.14 +0.0
436,688 + Pf6N2E2_369 0.14 +0.6
436,688 + Pf6N2E2_369 0.14 +0.1
436,689 - Pf6N2E2_369 0.14 -2.2
436,754 + Pf6N2E2_369 0.20 +1.3
436,844 + Pf6N2E2_369 0.28 +0.0
436,844 + Pf6N2E2_369 0.28 +0.8
436,845 - Pf6N2E2_369 0.28 +0.7
436,955 + Pf6N2E2_369 0.39 +0.0
437,405 + Pf6N2E2_369 0.80 -1.0
437,405 + Pf6N2E2_369 0.80 +1.2
437,405 + Pf6N2E2_369 0.80 -0.9
437,405 + Pf6N2E2_369 0.80 +0.8
437,406 - Pf6N2E2_369 0.80 +0.3
437,490 - Pf6N2E2_369 0.88 +0.0

Or see this region's nucleotide sequence