Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2829 and Pf6N2E2_2830 are separated by 10 nucleotides Pf6N2E2_2830 and Pf6N2E2_2831 are separated by 12 nucleotides Pf6N2E2_2831 and Pf6N2E2_2832 are separated by 152 nucleotides
Pf6N2E2_2829 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 3,318,565 to 3,318,957
_2829
Pf6N2E2_2830 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 3,318,968 to 3,319,768
_2830
Pf6N2E2_2831 - 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2), at 3,319,781 to 3,321,145
_2831
Pf6N2E2_2832 - dicarboxylic acid transporter PcaT, at 3,321,298 to 3,322,596
_2832
Position (kb)
3319
3320
3321
3322 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3318.919 kb on + strand at 3318.920 kb on - strand at 3318.938 kb on + strand at 3318.938 kb on + strand at 3319.054 kb on - strand, within Pf6N2E2_2830 at 3319.065 kb on + strand, within Pf6N2E2_2830 at 3319.331 kb on + strand, within Pf6N2E2_2830 at 3319.359 kb on - strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.507 kb on + strand, within Pf6N2E2_2830 at 3319.507 kb on + strand, within Pf6N2E2_2830 at 3319.731 kb on + strand at 3319.731 kb on + strand at 3320.706 kb on + strand, within Pf6N2E2_2831 at 3320.706 kb on + strand, within Pf6N2E2_2831 at 3320.828 kb on + strand, within Pf6N2E2_2831 at 3320.828 kb on + strand, within Pf6N2E2_2831 at 3320.838 kb on + strand, within Pf6N2E2_2831 at 3320.839 kb on - strand, within Pf6N2E2_2831 at 3320.839 kb on - strand, within Pf6N2E2_2831 at 3320.839 kb on - strand, within Pf6N2E2_2831 at 3320.950 kb on - strand, within Pf6N2E2_2831 at 3321.108 kb on + strand at 3321.108 kb on + strand at 3321.109 kb on - strand at 3321.109 kb on - strand at 3321.109 kb on - strand at 3321.145 kb on + strand at 3321.145 kb on + strand at 3321.145 kb on + strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.165 kb on - strand at 3321.317 kb on + strand at 3321.317 kb on + strand at 3321.380 kb on + strand at 3321.380 kb on + strand at 3321.380 kb on + strand at 3321.380 kb on + strand at 3321.381 kb on - strand at 3321.381 kb on - strand at 3321.381 kb on - strand at 3321.381 kb on - strand at 3321.431 kb on + strand, within Pf6N2E2_2832 at 3321.431 kb on + strand, within Pf6N2E2_2832 at 3321.431 kb on + strand, within Pf6N2E2_2832 at 3321.431 kb on + strand, within Pf6N2E2_2832 at 3321.432 kb on - strand, within Pf6N2E2_2832 at 3321.432 kb on - strand, within Pf6N2E2_2832 at 3321.432 kb on - strand, within Pf6N2E2_2832 at 3321.470 kb on + strand, within Pf6N2E2_2832 at 3321.471 kb on - strand, within Pf6N2E2_2832 at 3321.481 kb on + strand, within Pf6N2E2_2832 at 3321.481 kb on + strand, within Pf6N2E2_2832 at 3321.481 kb on + strand, within Pf6N2E2_2832 at 3321.482 kb on - strand, within Pf6N2E2_2832 at 3321.482 kb on - strand, within Pf6N2E2_2832 at 3321.482 kb on - strand, within Pf6N2E2_2832 at 3321.545 kb on + strand, within Pf6N2E2_2832 at 3321.545 kb on + strand, within Pf6N2E2_2832 at 3321.545 kb on + strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.546 kb on - strand, within Pf6N2E2_2832 at 3321.676 kb on - strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.728 kb on + strand, within Pf6N2E2_2832 at 3321.729 kb on - strand at 3321.729 kb on - strand, within Pf6N2E2_2832 at 3321.755 kb on + strand, within Pf6N2E2_2832 at 3321.755 kb on + strand, within Pf6N2E2_2832 at 3321.755 kb on + strand, within Pf6N2E2_2832 at 3321.756 kb on - strand, within Pf6N2E2_2832 at 3321.756 kb on - strand, within Pf6N2E2_2832 at 3321.756 kb on - strand, within Pf6N2E2_2832 at 3321.797 kb on + strand, within Pf6N2E2_2832 at 3321.797 kb on + strand, within Pf6N2E2_2832 at 3321.798 kb on - strand, within Pf6N2E2_2832 at 3321.798 kb on - strand, within Pf6N2E2_2832 at 3321.798 kb on - strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.809 kb on + strand, within Pf6N2E2_2832 at 3321.810 kb on - strand, within Pf6N2E2_2832 at 3321.810 kb on - strand, within Pf6N2E2_2832 at 3321.810 kb on - strand, within Pf6N2E2_2832 at 3321.810 kb on - strand, within Pf6N2E2_2832 at 3322.022 kb on + strand, within Pf6N2E2_2832 at 3322.023 kb on - strand, within Pf6N2E2_2832 at 3322.023 kb on - strand, within Pf6N2E2_2832 at 3322.109 kb on + strand, within Pf6N2E2_2832 at 3322.109 kb on + strand, within Pf6N2E2_2832
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 3,318,919 + -0.0 3,318,920 - -0.5 3,318,938 + +0.5 3,318,938 + +0.1 3,319,054 - Pf6N2E2_2830 0.11 -0.9 3,319,065 + Pf6N2E2_2830 0.12 +0.9 3,319,331 + Pf6N2E2_2830 0.45 +0.4 3,319,359 - Pf6N2E2_2830 0.49 -0.1 3,319,431 + Pf6N2E2_2830 0.58 -1.0 3,319,431 + Pf6N2E2_2830 0.58 -0.5 3,319,431 + Pf6N2E2_2830 0.58 -0.3 3,319,507 + Pf6N2E2_2830 0.67 +0.1 3,319,507 + Pf6N2E2_2830 0.67 -2.2 3,319,731 + +0.2 3,319,731 + -1.1 3,320,706 + Pf6N2E2_2831 0.68 -1.6 3,320,706 + Pf6N2E2_2831 0.68 +0.1 3,320,828 + Pf6N2E2_2831 0.77 -0.7 3,320,828 + Pf6N2E2_2831 0.77 -1.0 3,320,838 + Pf6N2E2_2831 0.77 -0.5 3,320,839 - Pf6N2E2_2831 0.78 -1.1 3,320,839 - Pf6N2E2_2831 0.78 -1.3 3,320,839 - Pf6N2E2_2831 0.78 +0.2 3,320,950 - Pf6N2E2_2831 0.86 +0.1 3,321,108 + +0.5 3,321,108 + +0.2 3,321,109 - +0.5 3,321,109 - -0.0 3,321,109 - -0.5 3,321,145 + +0.3 3,321,145 + +0.1 3,321,145 + +0.4 3,321,165 - -0.5 3,321,165 - +0.5 3,321,165 - -1.3 3,321,165 - -0.4 3,321,165 - -0.1 3,321,165 - +0.9 3,321,165 - -2.7 3,321,165 - +0.2 3,321,317 + +0.1 3,321,317 + -0.6 3,321,380 + -0.6 3,321,380 + -0.6 3,321,380 + +0.4 3,321,380 + +0.3 3,321,381 - +0.7 3,321,381 - -0.8 3,321,381 - +0.1 3,321,381 - -0.4 3,321,431 + Pf6N2E2_2832 0.10 +0.1 3,321,431 + Pf6N2E2_2832 0.10 +1.1 3,321,431 + Pf6N2E2_2832 0.10 +1.1 3,321,431 + Pf6N2E2_2832 0.10 -1.1 3,321,432 - Pf6N2E2_2832 0.10 +0.7 3,321,432 - Pf6N2E2_2832 0.10 -0.4 3,321,432 - Pf6N2E2_2832 0.10 +0.8 3,321,470 + Pf6N2E2_2832 0.13 +1.2 3,321,471 - Pf6N2E2_2832 0.13 +1.1 3,321,481 + Pf6N2E2_2832 0.14 +0.1 3,321,481 + Pf6N2E2_2832 0.14 -0.3 3,321,481 + Pf6N2E2_2832 0.14 -1.2 3,321,482 - Pf6N2E2_2832 0.14 -0.8 3,321,482 - Pf6N2E2_2832 0.14 +0.8 3,321,482 - Pf6N2E2_2832 0.14 +0.6 3,321,545 + Pf6N2E2_2832 0.19 -0.4 3,321,545 + Pf6N2E2_2832 0.19 +0.1 3,321,545 + Pf6N2E2_2832 0.19 +0.4 3,321,546 - Pf6N2E2_2832 0.19 +0.3 3,321,546 - Pf6N2E2_2832 0.19 -2.7 3,321,546 - Pf6N2E2_2832 0.19 -0.7 3,321,546 - Pf6N2E2_2832 0.19 -0.6 3,321,546 - Pf6N2E2_2832 0.19 -0.3 3,321,546 - Pf6N2E2_2832 0.19 -0.6 3,321,676 - Pf6N2E2_2832 0.29 -0.5 3,321,728 + Pf6N2E2_2832 0.33 +0.6 3,321,728 + Pf6N2E2_2832 0.33 +0.7 3,321,728 + Pf6N2E2_2832 0.33 -0.4 3,321,728 + Pf6N2E2_2832 0.33 +0.7 3,321,728 + Pf6N2E2_2832 0.33 -0.8 3,321,728 + Pf6N2E2_2832 0.33 +0.1 3,321,729 - +1.2 3,321,729 - Pf6N2E2_2832 0.33 +1.9 3,321,755 + Pf6N2E2_2832 0.35 +0.2 3,321,755 + Pf6N2E2_2832 0.35 -0.9 3,321,755 + Pf6N2E2_2832 0.35 -0.0 3,321,756 - Pf6N2E2_2832 0.35 +1.1 3,321,756 - Pf6N2E2_2832 0.35 -1.7 3,321,756 - Pf6N2E2_2832 0.35 -1.1 3,321,797 + Pf6N2E2_2832 0.38 +0.9 3,321,797 + Pf6N2E2_2832 0.38 -1.0 3,321,798 - Pf6N2E2_2832 0.38 +0.4 3,321,798 - Pf6N2E2_2832 0.38 -1.4 3,321,798 - Pf6N2E2_2832 0.38 -0.2 3,321,809 + Pf6N2E2_2832 0.39 +0.6 3,321,809 + Pf6N2E2_2832 0.39 -0.2 3,321,809 + Pf6N2E2_2832 0.39 -0.3 3,321,809 + Pf6N2E2_2832 0.39 -0.1 3,321,809 + Pf6N2E2_2832 0.39 +0.7 3,321,809 + Pf6N2E2_2832 0.39 -0.9 3,321,809 + Pf6N2E2_2832 0.39 +0.7 3,321,809 + Pf6N2E2_2832 0.39 +0.4 3,321,809 + Pf6N2E2_2832 0.39 -0.1 3,321,809 + Pf6N2E2_2832 0.39 +0.7 3,321,809 + Pf6N2E2_2832 0.39 +0.6 3,321,810 - Pf6N2E2_2832 0.39 +0.7 3,321,810 - Pf6N2E2_2832 0.39 +0.3 3,321,810 - Pf6N2E2_2832 0.39 -0.5 3,321,810 - Pf6N2E2_2832 0.39 -0.3 3,322,022 + Pf6N2E2_2832 0.56 +0.9 3,322,023 - Pf6N2E2_2832 0.56 +0.4 3,322,023 - Pf6N2E2_2832 0.56 -0.9 3,322,109 + Pf6N2E2_2832 0.62 -0.0 3,322,109 + Pf6N2E2_2832 0.62 +0.6
Or see this region's nucleotide sequence