Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_195 and Pf6N2E2_196 are separated by 205 nucleotides Pf6N2E2_196 and Pf6N2E2_197 overlap by 4 nucleotides Pf6N2E2_197 and Pf6N2E2_198 are separated by 106 nucleotides Pf6N2E2_198 and Pf6N2E2_199 are separated by 9 nucleotides Pf6N2E2_199 and Pf6N2E2_200 are separated by 60 nucleotides
Pf6N2E2_195 - hypothetical protein, at 247,566 to 249,515
_195
Pf6N2E2_196 - hypothetical protein, at 249,721 to 249,948
_196
Pf6N2E2_197 - Death on curing protein, Doc toxin, at 249,945 to 250,262
_197
Pf6N2E2_198 - hypothetical protein, at 250,369 to 250,527
_198
Pf6N2E2_199 - hypothetical protein, at 250,537 to 250,800
_199
Pf6N2E2_200 - Possible glycosyltransferase, at 250,861 to 251,967
_200
Position (kb)
250
251 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 249.472 kb on + strand at 249.472 kb on + strand at 249.472 kb on + strand at 249.472 kb on + strand at 249.472 kb on + strand at 249.472 kb on + strand at 249.472 kb on + strand at 249.472 kb on + strand at 249.473 kb on - strand at 249.473 kb on - strand at 249.566 kb on + strand at 249.566 kb on + strand at 249.567 kb on - strand at 249.567 kb on - strand at 249.576 kb on + strand at 249.576 kb on + strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.577 kb on - strand at 249.677 kb on - strand at 249.717 kb on + strand at 249.717 kb on + strand at 249.803 kb on - strand, within Pf6N2E2_196 at 249.991 kb on + strand, within Pf6N2E2_197 at 249.991 kb on + strand, within Pf6N2E2_197 at 249.991 kb on + strand, within Pf6N2E2_197 at 249.992 kb on - strand, within Pf6N2E2_197 at 250.008 kb on + strand, within Pf6N2E2_197 at 250.009 kb on - strand, within Pf6N2E2_197 at 250.009 kb on - strand, within Pf6N2E2_197 at 250.012 kb on - strand, within Pf6N2E2_197 at 250.012 kb on - strand, within Pf6N2E2_197 at 250.071 kb on + strand, within Pf6N2E2_197 at 250.071 kb on + strand, within Pf6N2E2_197 at 250.071 kb on + strand, within Pf6N2E2_197 at 250.071 kb on + strand, within Pf6N2E2_197 at 250.072 kb on - strand, within Pf6N2E2_197 at 250.072 kb on - strand, within Pf6N2E2_197 at 250.072 kb on - strand, within Pf6N2E2_197 at 250.073 kb on + strand, within Pf6N2E2_197 at 250.073 kb on + strand, within Pf6N2E2_197 at 250.073 kb on + strand, within Pf6N2E2_197 at 250.073 kb on + strand, within Pf6N2E2_197 at 250.073 kb on + strand, within Pf6N2E2_197 at 250.074 kb on - strand, within Pf6N2E2_197 at 250.074 kb on - strand, within Pf6N2E2_197 at 250.074 kb on - strand, within Pf6N2E2_197 at 250.074 kb on - strand, within Pf6N2E2_197 at 250.074 kb on - strand, within Pf6N2E2_197 at 250.103 kb on + strand, within Pf6N2E2_197 at 250.104 kb on - strand, within Pf6N2E2_197 at 250.114 kb on - strand, within Pf6N2E2_197 at 250.171 kb on - strand, within Pf6N2E2_197 at 250.182 kb on + strand, within Pf6N2E2_197 at 250.182 kb on + strand, within Pf6N2E2_197 at 250.182 kb on + strand, within Pf6N2E2_197 at 250.182 kb on + strand, within Pf6N2E2_197 at 250.211 kb on - strand, within Pf6N2E2_197 at 250.429 kb on + strand, within Pf6N2E2_198 at 250.430 kb on - strand, within Pf6N2E2_198 at 250.430 kb on - strand, within Pf6N2E2_198 at 250.430 kb on - strand, within Pf6N2E2_198 at 250.455 kb on + strand, within Pf6N2E2_198 at 250.455 kb on + strand, within Pf6N2E2_198 at 250.455 kb on + strand, within Pf6N2E2_198 at 250.456 kb on - strand, within Pf6N2E2_198 at 250.456 kb on - strand, within Pf6N2E2_198 at 250.456 kb on - strand, within Pf6N2E2_198 at 250.480 kb on + strand, within Pf6N2E2_198 at 250.748 kb on + strand, within Pf6N2E2_199 at 250.749 kb on - strand, within Pf6N2E2_199 at 250.749 kb on - strand, within Pf6N2E2_199 at 250.749 kb on - strand, within Pf6N2E2_199 at 250.777 kb on + strand at 250.778 kb on - strand at 250.787 kb on + strand at 250.787 kb on + strand at 250.809 kb on + strand at 250.810 kb on - strand at 250.843 kb on + strand at 251.087 kb on + strand, within Pf6N2E2_200 at 251.088 kb on - strand, within Pf6N2E2_200 at 251.222 kb on - strand, within Pf6N2E2_200 at 251.222 kb on - strand, within Pf6N2E2_200 at 251.422 kb on + strand, within Pf6N2E2_200 at 251.423 kb on - strand, within Pf6N2E2_200 at 251.476 kb on + strand, within Pf6N2E2_200 at 251.476 kb on + strand, within Pf6N2E2_200 at 251.477 kb on - strand, within Pf6N2E2_200
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 249,472 + +0.4 249,472 + +0.1 249,472 + +1.0 249,472 + -0.7 249,472 + -0.2 249,472 + +0.3 249,472 + -0.3 249,472 + +0.7 249,473 - +0.3 249,473 - -0.3 249,566 + -0.7 249,566 + +0.2 249,567 - -0.5 249,567 - -0.0 249,576 + -0.2 249,576 + -1.2 249,577 - -0.1 249,577 - +0.4 249,577 - +0.6 249,577 - +0.5 249,577 - -1.0 249,577 - -1.1 249,577 - -0.0 249,677 - -0.7 249,717 + -0.4 249,717 + +0.0 249,803 - Pf6N2E2_196 0.36 +0.0 249,991 + Pf6N2E2_197 0.14 +0.2 249,991 + Pf6N2E2_197 0.14 -0.4 249,991 + Pf6N2E2_197 0.14 +1.0 249,992 - Pf6N2E2_197 0.15 +0.7 250,008 + Pf6N2E2_197 0.20 +1.1 250,009 - Pf6N2E2_197 0.20 -0.0 250,009 - Pf6N2E2_197 0.20 +1.0 250,012 - Pf6N2E2_197 0.21 -0.3 250,012 - Pf6N2E2_197 0.21 -1.0 250,071 + Pf6N2E2_197 0.40 -0.0 250,071 + Pf6N2E2_197 0.40 +0.1 250,071 + Pf6N2E2_197 0.40 +1.3 250,071 + Pf6N2E2_197 0.40 +0.0 250,072 - Pf6N2E2_197 0.40 -0.1 250,072 - Pf6N2E2_197 0.40 +1.7 250,072 - Pf6N2E2_197 0.40 -0.4 250,073 + Pf6N2E2_197 0.40 +0.4 250,073 + Pf6N2E2_197 0.40 -0.5 250,073 + Pf6N2E2_197 0.40 -0.3 250,073 + Pf6N2E2_197 0.40 -0.8 250,073 + Pf6N2E2_197 0.40 -1.4 250,074 - Pf6N2E2_197 0.41 -0.7 250,074 - Pf6N2E2_197 0.41 -1.0 250,074 - Pf6N2E2_197 0.41 -1.1 250,074 - Pf6N2E2_197 0.41 +0.5 250,074 - Pf6N2E2_197 0.41 -0.4 250,103 + Pf6N2E2_197 0.50 -0.1 250,104 - Pf6N2E2_197 0.50 -1.2 250,114 - Pf6N2E2_197 0.53 +0.5 250,171 - Pf6N2E2_197 0.71 +1.0 250,182 + Pf6N2E2_197 0.75 -0.7 250,182 + Pf6N2E2_197 0.75 -1.2 250,182 + Pf6N2E2_197 0.75 +1.0 250,182 + Pf6N2E2_197 0.75 -0.3 250,211 - Pf6N2E2_197 0.84 -2.0 250,429 + Pf6N2E2_198 0.38 -0.5 250,430 - Pf6N2E2_198 0.38 -1.0 250,430 - Pf6N2E2_198 0.38 -0.1 250,430 - Pf6N2E2_198 0.38 -0.2 250,455 + Pf6N2E2_198 0.54 -0.8 250,455 + Pf6N2E2_198 0.54 -0.7 250,455 + Pf6N2E2_198 0.54 -0.2 250,456 - Pf6N2E2_198 0.55 -0.7 250,456 - Pf6N2E2_198 0.55 -1.6 250,456 - Pf6N2E2_198 0.55 -1.4 250,480 + Pf6N2E2_198 0.70 +0.1 250,748 + Pf6N2E2_199 0.80 +0.4 250,749 - Pf6N2E2_199 0.80 -0.1 250,749 - Pf6N2E2_199 0.80 +0.4 250,749 - Pf6N2E2_199 0.80 +0.5 250,777 + +0.0 250,778 - +1.3 250,787 + +1.8 250,787 + -0.3 250,809 + -0.2 250,810 - +0.7 250,843 + -1.4 251,087 + Pf6N2E2_200 0.20 +0.6 251,088 - Pf6N2E2_200 0.21 -0.7 251,222 - Pf6N2E2_200 0.33 -0.8 251,222 - Pf6N2E2_200 0.33 -0.0 251,422 + Pf6N2E2_200 0.51 -1.0 251,423 - Pf6N2E2_200 0.51 +0.2 251,476 + Pf6N2E2_200 0.56 +0.7 251,476 + Pf6N2E2_200 0.56 -0.7 251,477 - Pf6N2E2_200 0.56 -0.4
Or see this region's nucleotide sequence