Experiment: Calcium chloride 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1459 and Pf6N2E2_1460 are separated by 2 nucleotides Pf6N2E2_1460 and Pf6N2E2_1461 are separated by 472 nucleotides
Pf6N2E2_1459 - Glycine cleavage system transcriptional activator GcvA, at 1,707,865 to 1,708,770
_1459
Pf6N2E2_1460 - Epoxide hydrolase (EC 3.3.2.9), at 1,708,773 to 1,709,918
_1460
Pf6N2E2_1461 - hypothetical protein, at 1,710,391 to 1,710,672
_1461
Position (kb)
1708
1709
1710 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1707.845 kb on + strand at 1707.845 kb on + strand at 1707.846 kb on - strand at 1707.854 kb on - strand at 1707.986 kb on + strand, within Pf6N2E2_1459 at 1707.986 kb on + strand, within Pf6N2E2_1459 at 1707.987 kb on - strand, within Pf6N2E2_1459 at 1707.987 kb on - strand, within Pf6N2E2_1459 at 1708.292 kb on + strand, within Pf6N2E2_1459 at 1708.293 kb on - strand, within Pf6N2E2_1459 at 1708.293 kb on - strand, within Pf6N2E2_1459 at 1708.293 kb on - strand, within Pf6N2E2_1459 at 1708.361 kb on + strand, within Pf6N2E2_1459 at 1708.361 kb on + strand, within Pf6N2E2_1459 at 1708.361 kb on + strand, within Pf6N2E2_1459 at 1708.361 kb on + strand, within Pf6N2E2_1459 at 1708.362 kb on - strand, within Pf6N2E2_1459 at 1708.362 kb on - strand, within Pf6N2E2_1459 at 1708.362 kb on - strand, within Pf6N2E2_1459 at 1708.672 kb on + strand, within Pf6N2E2_1459 at 1708.673 kb on - strand, within Pf6N2E2_1459 at 1708.769 kb on - strand at 1708.907 kb on - strand, within Pf6N2E2_1460 at 1709.019 kb on - strand, within Pf6N2E2_1460 at 1709.229 kb on + strand, within Pf6N2E2_1460 at 1709.229 kb on + strand, within Pf6N2E2_1460 at 1709.275 kb on - strand, within Pf6N2E2_1460 at 1709.275 kb on - strand, within Pf6N2E2_1460 at 1709.373 kb on + strand, within Pf6N2E2_1460 at 1709.374 kb on - strand, within Pf6N2E2_1460 at 1709.640 kb on + strand, within Pf6N2E2_1460 at 1709.679 kb on + strand, within Pf6N2E2_1460 at 1709.680 kb on - strand, within Pf6N2E2_1460 at 1710.143 kb on + strand at 1710.143 kb on + strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.144 kb on - strand at 1710.145 kb on + strand at 1710.145 kb on + strand at 1710.145 kb on + strand at 1710.145 kb on + strand at 1710.145 kb on + strand at 1710.145 kb on + strand at 1710.146 kb on - strand at 1710.146 kb on - strand at 1710.146 kb on - strand at 1710.205 kb on + strand at 1710.206 kb on - strand at 1710.206 kb on - strand at 1710.206 kb on - strand at 1710.252 kb on - strand at 1710.338 kb on + strand at 1710.339 kb on - strand at 1710.383 kb on - strand at 1710.383 kb on - strand at 1710.383 kb on - strand at 1710.391 kb on - strand at 1710.391 kb on - strand at 1710.391 kb on - strand at 1710.533 kb on - strand, within Pf6N2E2_1461 at 1710.662 kb on + strand at 1710.769 kb on + strand at 1710.828 kb on + strand at 1710.828 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Calcium chloride 0.0125 M remove 1,707,845 + +0.6 1,707,845 + +0.4 1,707,846 - -0.3 1,707,854 - -0.1 1,707,986 + Pf6N2E2_1459 0.13 -0.3 1,707,986 + Pf6N2E2_1459 0.13 +0.4 1,707,987 - Pf6N2E2_1459 0.13 -1.2 1,707,987 - Pf6N2E2_1459 0.13 -0.9 1,708,292 + Pf6N2E2_1459 0.47 -0.3 1,708,293 - Pf6N2E2_1459 0.47 +1.4 1,708,293 - Pf6N2E2_1459 0.47 -0.0 1,708,293 - Pf6N2E2_1459 0.47 -0.6 1,708,361 + Pf6N2E2_1459 0.55 +0.1 1,708,361 + Pf6N2E2_1459 0.55 +1.9 1,708,361 + Pf6N2E2_1459 0.55 +0.7 1,708,361 + Pf6N2E2_1459 0.55 -1.2 1,708,362 - Pf6N2E2_1459 0.55 -0.0 1,708,362 - Pf6N2E2_1459 0.55 +0.3 1,708,362 - Pf6N2E2_1459 0.55 +0.4 1,708,672 + Pf6N2E2_1459 0.89 +0.0 1,708,673 - Pf6N2E2_1459 0.89 -0.6 1,708,769 - +0.7 1,708,907 - Pf6N2E2_1460 0.12 -0.2 1,709,019 - Pf6N2E2_1460 0.21 -0.8 1,709,229 + Pf6N2E2_1460 0.40 -0.9 1,709,229 + Pf6N2E2_1460 0.40 -3.5 1,709,275 - Pf6N2E2_1460 0.44 -0.2 1,709,275 - Pf6N2E2_1460 0.44 -0.6 1,709,373 + Pf6N2E2_1460 0.52 +0.1 1,709,374 - Pf6N2E2_1460 0.52 -0.1 1,709,640 + Pf6N2E2_1460 0.76 -0.8 1,709,679 + Pf6N2E2_1460 0.79 -1.1 1,709,680 - Pf6N2E2_1460 0.79 +1.4 1,710,143 + -0.0 1,710,143 + +0.2 1,710,144 - +0.1 1,710,144 - -0.7 1,710,144 - +0.0 1,710,144 - +0.5 1,710,144 - -0.1 1,710,144 - -0.2 1,710,144 - +0.3 1,710,144 - -0.0 1,710,144 - +0.0 1,710,145 + +0.6 1,710,145 + -0.4 1,710,145 + -0.4 1,710,145 + -0.6 1,710,145 + -0.6 1,710,145 + -1.0 1,710,146 - -0.5 1,710,146 - +0.0 1,710,146 - -0.9 1,710,205 + -0.5 1,710,206 - +0.5 1,710,206 - +0.1 1,710,206 - -0.3 1,710,252 - +1.4 1,710,338 + +1.3 1,710,339 - -0.5 1,710,383 - +0.7 1,710,383 - -0.2 1,710,383 - -0.4 1,710,391 - -0.6 1,710,391 - -1.1 1,710,391 - -0.0 1,710,533 - Pf6N2E2_1461 0.50 -0.1 1,710,662 + -1.2 1,710,769 + +0.0 1,710,828 + +0.8 1,710,828 + -0.8
Or see this region's nucleotide sequence