Experiment: Pyruvate-Sulfate (60-15mM) with Selenate 750uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1331 and selD overlap by 11 nucleotides selD and DVU1333 are separated by 27 nucleotides DVU1333 and tRNA-Leu overlap by 18 nucleotides tRNA-Leu and tig are separated by 78 nucleotides
DVU1331: DVU1331 - transcriptional regulator, LysR family (TIGR), at 1,407,515 to 1,408,441
DVU1331
DVU1332: selD - selenide, water dikinase, selenocysteine-containing (TIGR), at 1,408,431 to 1,409,486
selD
DVU1333: DVU1333 - hypothetical protein (TIGR), at 1,409,514 to 1,409,639
DVU1333
DVU_tRNA-Leu-3: tRNA-Leu - tRNA with anticodon TAG for Leu (VIMSS-AUTO), at 1,409,622 to 1,409,706
tRNA-Leu
DVU1334: tig - trigger factor (TIGR), at 1,409,785 to 1,411,086
tig
Position (kb)
1408
1409
1410 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1407.457 kb on + strand at 1407.551 kb on + strand at 1407.581 kb on + strand at 1407.581 kb on + strand at 1407.589 kb on - strand at 1407.615 kb on + strand, within DVU1331 at 1407.779 kb on + strand, within DVU1331 at 1407.837 kb on + strand, within DVU1331 at 1407.929 kb on - strand, within DVU1331 at 1407.972 kb on + strand, within DVU1331 at 1408.061 kb on - strand, within DVU1331 at 1408.194 kb on - strand, within DVU1331 at 1408.199 kb on - strand, within DVU1331 at 1408.199 kb on - strand, within DVU1331 at 1408.244 kb on - strand, within DVU1331 at 1408.306 kb on + strand, within DVU1331 at 1408.314 kb on - strand, within DVU1331 at 1408.662 kb on - strand, within selD at 1408.709 kb on - strand, within selD at 1408.720 kb on - strand, within selD at 1408.722 kb on - strand, within selD at 1408.830 kb on + strand, within selD at 1408.838 kb on - strand, within selD at 1408.838 kb on - strand, within selD at 1408.852 kb on - strand, within selD at 1408.895 kb on + strand, within selD at 1408.928 kb on + strand, within selD at 1408.964 kb on + strand, within selD at 1409.011 kb on + strand, within selD at 1409.057 kb on + strand, within selD at 1409.060 kb on - strand, within selD at 1409.070 kb on - strand, within selD at 1409.070 kb on - strand, within selD at 1409.075 kb on + strand, within selD at 1409.125 kb on + strand, within selD at 1409.127 kb on - strand, within selD at 1409.219 kb on - strand, within selD at 1409.395 kb on - strand at 1409.450 kb on + strand at 1409.471 kb on + strand at 1409.480 kb on + strand at 1409.491 kb on + strand at 1409.706 kb on + strand at 1409.713 kb on + strand at 1409.739 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Sulfate (60-15mM) with Selenate 750uM remove 1,407,457 + +0.6 1,407,551 + -0.0 1,407,581 + +0.0 1,407,581 + +1.6 1,407,589 - +0.4 1,407,615 + DVU1331 0.11 -0.2 1,407,779 + DVU1331 0.28 +0.5 1,407,837 + DVU1331 0.35 -1.0 1,407,929 - DVU1331 0.45 -0.2 1,407,972 + DVU1331 0.49 +0.2 1,408,061 - DVU1331 0.59 +0.4 1,408,194 - DVU1331 0.73 +0.5 1,408,199 - DVU1331 0.74 +0.7 1,408,199 - DVU1331 0.74 -0.5 1,408,244 - DVU1331 0.79 -1.0 1,408,306 + DVU1331 0.85 -0.5 1,408,314 - DVU1331 0.86 -0.1 1,408,662 - selD DVU1332 0.22 -4.8 1,408,709 - selD DVU1332 0.26 -0.7 1,408,720 - selD DVU1332 0.27 -0.6 1,408,722 - selD DVU1332 0.28 -0.9 1,408,830 + selD DVU1332 0.38 +0.6 1,408,838 - selD DVU1332 0.39 +0.3 1,408,838 - selD DVU1332 0.39 +0.6 1,408,852 - selD DVU1332 0.40 -0.6 1,408,895 + selD DVU1332 0.44 -0.6 1,408,928 + selD DVU1332 0.47 -1.8 1,408,964 + selD DVU1332 0.50 -0.4 1,409,011 + selD DVU1332 0.55 -1.3 1,409,057 + selD DVU1332 0.59 -0.4 1,409,060 - selD DVU1332 0.60 -2.2 1,409,070 - selD DVU1332 0.61 -1.7 1,409,070 - selD DVU1332 0.61 -0.4 1,409,075 + selD DVU1332 0.61 -0.2 1,409,125 + selD DVU1332 0.66 +0.2 1,409,127 - selD DVU1332 0.66 -1.5 1,409,219 - selD DVU1332 0.75 -0.2 1,409,395 - -0.1 1,409,450 + -0.2 1,409,471 + -0.3 1,409,480 + +1.9 1,409,491 + +0.3 1,409,706 + +0.3 1,409,713 + +0.5 1,409,739 - -0.7
Or see this region's nucleotide sequence