Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS23645 and HSERO_RS23650 are separated by 12 nucleotides HSERO_RS23650 and HSERO_RS23655 overlap by 8 nucleotides HSERO_RS23655 and HSERO_RS23660 overlap by 4 nucleotides HSERO_RS23660 and HSERO_RS23665 are separated by 182 nucleotides
HSERO_RS23645: HSERO_RS23645 - glycerol-3-phosphate dehydrogenase, at 5,420,023 to 5,421,627
_RS23645
HSERO_RS23650: HSERO_RS23650 - phosphonate ABC transporter permease, at 5,421,640 to 5,422,473
_RS23650
HSERO_RS23655: HSERO_RS23655 - phosphonate ABC transporter permease, at 5,422,466 to 5,423,269
_RS23655
HSERO_RS23660: HSERO_RS23660 - phosphonate ABC transporter ATP-binding protein, at 5,423,266 to 5,424,036
_RS23660
HSERO_RS23665: HSERO_RS23665 - phosphonate ABC transporter substrate-binding protein, at 5,424,219 to 5,425,208
_RS23665
Position (kb) 5422
5423
5424 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5421.480 kb on - strand at 5421.586 kb on - strand at 5421.777 kb on - strand, within HSERO_RS23650 at 5422.060 kb on - strand, within HSERO_RS23650 at 5422.285 kb on + strand, within HSERO_RS23650 at 5422.354 kb on - strand, within HSERO_RS23650 at 5422.393 kb on + strand at 5422.535 kb on + strand at 5422.635 kb on - strand, within HSERO_RS23655 at 5423.296 kb on - strand at 5423.386 kb on - strand, within HSERO_RS23660 at 5423.613 kb on + strand, within HSERO_RS23660 at 5423.629 kb on - strand, within HSERO_RS23660 at 5423.639 kb on - strand, within HSERO_RS23660 at 5423.703 kb on + strand, within HSERO_RS23660 at 5423.970 kb on + strand at 5424.034 kb on - strand at 5424.266 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 5,421,480 - -0.2 5,421,586 - -0.0 5,421,777 - HSERO_RS23650 0.16 +0.2 5,422,060 - HSERO_RS23650 0.50 -0.1 5,422,285 + HSERO_RS23650 0.77 +0.0 5,422,354 - HSERO_RS23650 0.86 -1.2 5,422,393 + -0.0 5,422,535 + -1.2 5,422,635 - HSERO_RS23655 0.21 +0.3 5,423,296 - -0.2 5,423,386 - HSERO_RS23660 0.16 -3.9 5,423,613 + HSERO_RS23660 0.45 +0.2 5,423,629 - HSERO_RS23660 0.47 +1.3 5,423,639 - HSERO_RS23660 0.48 +0.8 5,423,703 + HSERO_RS23660 0.57 -0.0 5,423,970 + +0.4 5,424,034 - +0.3 5,424,266 - -0.3
Or see this region's nucleotide sequence