Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22970 and HSERO_RS22975 are separated by 173 nucleotides HSERO_RS22975 and HSERO_RS22980 are separated by 155 nucleotides HSERO_RS22980 and HSERO_RS22985 overlap by 4 nucleotides
HSERO_RS22970: HSERO_RS22970 - membrane protein, at 5,269,785 to 5,271,455
_RS22970
HSERO_RS22975: HSERO_RS22975 - chemotaxis protein, at 5,271,629 to 5,273,191
_RS22975
HSERO_RS22980: HSERO_RS22980 - membrane protein, at 5,273,347 to 5,274,183
_RS22980
HSERO_RS22985: HSERO_RS22985 - MFS transporter, at 5,274,180 to 5,275,421
_RS22985
Position (kb)
5271
5272
5273
5274 Strain fitness (log2 ratio)
-2
-1
0
1 at 5270.648 kb on + strand, within HSERO_RS22970 at 5270.682 kb on + strand, within HSERO_RS22970 at 5270.703 kb on - strand, within HSERO_RS22970 at 5270.775 kb on + strand, within HSERO_RS22970 at 5270.798 kb on - strand, within HSERO_RS22970 at 5270.905 kb on + strand, within HSERO_RS22970 at 5270.991 kb on - strand, within HSERO_RS22970 at 5271.063 kb on + strand, within HSERO_RS22970 at 5271.087 kb on + strand, within HSERO_RS22970 at 5271.228 kb on - strand, within HSERO_RS22970 at 5271.310 kb on + strand at 5271.341 kb on - strand at 5271.361 kb on + strand at 5271.397 kb on + strand at 5271.425 kb on - strand at 5271.507 kb on + strand at 5271.531 kb on + strand at 5271.546 kb on + strand at 5271.796 kb on + strand, within HSERO_RS22975 at 5271.816 kb on - strand, within HSERO_RS22975 at 5271.902 kb on - strand, within HSERO_RS22975 at 5271.930 kb on + strand, within HSERO_RS22975 at 5271.943 kb on - strand, within HSERO_RS22975 at 5271.980 kb on - strand at 5271.990 kb on - strand, within HSERO_RS22975 at 5272.115 kb on - strand, within HSERO_RS22975 at 5272.137 kb on + strand, within HSERO_RS22975 at 5272.149 kb on + strand, within HSERO_RS22975 at 5272.198 kb on + strand, within HSERO_RS22975 at 5272.294 kb on + strand, within HSERO_RS22975 at 5272.302 kb on - strand, within HSERO_RS22975 at 5272.338 kb on + strand, within HSERO_RS22975 at 5272.401 kb on + strand, within HSERO_RS22975 at 5272.426 kb on - strand, within HSERO_RS22975 at 5272.433 kb on + strand, within HSERO_RS22975 at 5272.617 kb on + strand, within HSERO_RS22975 at 5272.641 kb on - strand, within HSERO_RS22975 at 5272.654 kb on + strand, within HSERO_RS22975 at 5272.691 kb on + strand, within HSERO_RS22975 at 5272.743 kb on + strand, within HSERO_RS22975 at 5272.751 kb on - strand, within HSERO_RS22975 at 5272.767 kb on + strand, within HSERO_RS22975 at 5272.767 kb on + strand, within HSERO_RS22975 at 5272.805 kb on - strand, within HSERO_RS22975 at 5272.887 kb on - strand, within HSERO_RS22975 at 5272.984 kb on + strand, within HSERO_RS22975 at 5273.073 kb on + strand at 5273.073 kb on + strand at 5273.137 kb on + strand at 5273.149 kb on + strand at 5273.218 kb on - strand at 5273.375 kb on + strand at 5273.500 kb on + strand, within HSERO_RS22980 at 5273.556 kb on + strand, within HSERO_RS22980 at 5273.677 kb on - strand, within HSERO_RS22980 at 5273.762 kb on + strand, within HSERO_RS22980 at 5273.824 kb on - strand, within HSERO_RS22980 at 5273.840 kb on + strand, within HSERO_RS22980 at 5273.840 kb on + strand, within HSERO_RS22980 at 5274.014 kb on - strand, within HSERO_RS22980 at 5274.014 kb on - strand, within HSERO_RS22980 at 5274.140 kb on + strand at 5274.148 kb on - strand at 5274.158 kb on - strand at 5274.175 kb on - strand at 5274.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 5,270,648 + HSERO_RS22970 0.52 +0.2 5,270,682 + HSERO_RS22970 0.54 +0.3 5,270,703 - HSERO_RS22970 0.55 -0.0 5,270,775 + HSERO_RS22970 0.59 +0.3 5,270,798 - HSERO_RS22970 0.61 +0.3 5,270,905 + HSERO_RS22970 0.67 +0.5 5,270,991 - HSERO_RS22970 0.72 -0.2 5,271,063 + HSERO_RS22970 0.76 -0.1 5,271,087 + HSERO_RS22970 0.78 +0.2 5,271,228 - HSERO_RS22970 0.86 -0.5 5,271,310 + +0.1 5,271,341 - -0.3 5,271,361 + -0.4 5,271,397 + +0.9 5,271,425 - -0.0 5,271,507 + +0.0 5,271,531 + -0.5 5,271,546 + +0.4 5,271,796 + HSERO_RS22975 0.11 -1.9 5,271,816 - HSERO_RS22975 0.12 +0.1 5,271,902 - HSERO_RS22975 0.17 -1.3 5,271,930 + HSERO_RS22975 0.19 +0.1 5,271,943 - HSERO_RS22975 0.20 -0.1 5,271,980 - -1.9 5,271,990 - HSERO_RS22975 0.23 -0.3 5,272,115 - HSERO_RS22975 0.31 -0.3 5,272,137 + HSERO_RS22975 0.33 -0.1 5,272,149 + HSERO_RS22975 0.33 -0.8 5,272,198 + HSERO_RS22975 0.36 -0.2 5,272,294 + HSERO_RS22975 0.43 -0.2 5,272,302 - HSERO_RS22975 0.43 +0.2 5,272,338 + HSERO_RS22975 0.45 +0.3 5,272,401 + HSERO_RS22975 0.49 -0.1 5,272,426 - HSERO_RS22975 0.51 +0.6 5,272,433 + HSERO_RS22975 0.51 -0.1 5,272,617 + HSERO_RS22975 0.63 +0.3 5,272,641 - HSERO_RS22975 0.65 -1.1 5,272,654 + HSERO_RS22975 0.66 +0.3 5,272,691 + HSERO_RS22975 0.68 -0.1 5,272,743 + HSERO_RS22975 0.71 -0.1 5,272,751 - HSERO_RS22975 0.72 +0.5 5,272,767 + HSERO_RS22975 0.73 -0.0 5,272,767 + HSERO_RS22975 0.73 +0.2 5,272,805 - HSERO_RS22975 0.75 -0.0 5,272,887 - HSERO_RS22975 0.80 -1.4 5,272,984 + HSERO_RS22975 0.87 -0.4 5,273,073 + -0.2 5,273,073 + -0.2 5,273,137 + -0.2 5,273,149 + +0.0 5,273,218 - -0.4 5,273,375 + -0.6 5,273,500 + HSERO_RS22980 0.18 -0.4 5,273,556 + HSERO_RS22980 0.25 -1.0 5,273,677 - HSERO_RS22980 0.39 -0.5 5,273,762 + HSERO_RS22980 0.50 -0.5 5,273,824 - HSERO_RS22980 0.57 +0.1 5,273,840 + HSERO_RS22980 0.59 -1.3 5,273,840 + HSERO_RS22980 0.59 -0.9 5,274,014 - HSERO_RS22980 0.80 -1.3 5,274,014 - HSERO_RS22980 0.80 -1.1 5,274,140 + +0.1 5,274,148 - -0.0 5,274,158 - -0.7 5,274,175 - +0.3 5,274,180 - +0.1
Or see this region's nucleotide sequence