Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22345 and HSERO_RS22350 are separated by 109 nucleotides HSERO_RS22350 and HSERO_RS22355 are separated by 15 nucleotides HSERO_RS22355 and HSERO_RS22360 are separated by 1 nucleotides HSERO_RS22360 and HSERO_RS22365 overlap by 4 nucleotides
HSERO_RS22345: HSERO_RS22345 - urea carboxylase, at 5,111,553 to 5,115,176
_RS22345
HSERO_RS22350: HSERO_RS22350 - urea carboxylase, at 5,115,286 to 5,115,921
_RS22350
HSERO_RS22355: HSERO_RS22355 - urea carboxylase, at 5,115,937 to 5,116,668
_RS22355
HSERO_RS22360: HSERO_RS22360 - lauroyl acyltransferase, at 5,116,670 to 5,117,446
_RS22360
HSERO_RS22365: HSERO_RS22365 - lipid kinase, at 5,117,443 to 5,118,258
_RS22365
Position (kb) 5115
5116
5117 Strain fitness (log2 ratio) -1
0
1
2 at 5115.074 kb on + strand at 5115.251 kb on - strand at 5115.255 kb on + strand at 5115.255 kb on + strand at 5115.263 kb on - strand at 5115.272 kb on + strand at 5115.295 kb on + strand at 5115.297 kb on - strand at 5115.650 kb on + strand, within HSERO_RS22350 at 5116.306 kb on - strand, within HSERO_RS22355 at 5116.344 kb on + strand, within HSERO_RS22355 at 5116.360 kb on - strand, within HSERO_RS22355 at 5116.428 kb on - strand, within HSERO_RS22355 at 5116.473 kb on - strand, within HSERO_RS22355 at 5116.666 kb on - strand at 5116.907 kb on + strand, within HSERO_RS22360 at 5117.008 kb on + strand, within HSERO_RS22360 at 5117.352 kb on - strand, within HSERO_RS22360 at 5117.470 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 5,115,074 + -0.4 5,115,251 - +0.7 5,115,255 + -0.3 5,115,255 + +1.4 5,115,263 - -0.1 5,115,272 + +0.3 5,115,295 + -0.4 5,115,297 - +0.7 5,115,650 + HSERO_RS22350 0.57 +0.2 5,116,306 - HSERO_RS22355 0.50 +1.9 5,116,344 + HSERO_RS22355 0.56 +0.1 5,116,360 - HSERO_RS22355 0.58 +0.8 5,116,428 - HSERO_RS22355 0.67 +0.2 5,116,473 - HSERO_RS22355 0.73 +0.6 5,116,666 - +0.5 5,116,907 + HSERO_RS22360 0.31 +0.1 5,117,008 + HSERO_RS22360 0.44 -0.7 5,117,352 - HSERO_RS22360 0.88 +0.3 5,117,470 - +0.0
Or see this region's nucleotide sequence