Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS22145 and HSERO_RS22150 are separated by 135 nucleotides HSERO_RS22150 and HSERO_RS22155 are separated by 163 nucleotides HSERO_RS22155 and HSERO_RS22160 overlap by 11 nucleotides
HSERO_RS22145: HSERO_RS22145 - hypothetical protein, at 5,064,419 to 5,064,760
_RS22145
HSERO_RS22150: HSERO_RS22150 - membrane protein, at 5,064,896 to 5,065,477
_RS22150
HSERO_RS22155: HSERO_RS22155 - hypothetical protein, at 5,065,641 to 5,066,162
_RS22155
HSERO_RS22160: HSERO_RS22160 - cation transporter, at 5,066,152 to 5,069,373
_RS22160
Position (kb) 5065
5066
5067 Strain fitness (log2 ratio) -1
0
1 at 5064.723 kb on + strand, within HSERO_RS22145 at 5064.723 kb on + strand, within HSERO_RS22145 at 5064.789 kb on - strand at 5065.003 kb on + strand, within HSERO_RS22150 at 5065.008 kb on + strand, within HSERO_RS22150 at 5065.115 kb on - strand, within HSERO_RS22150 at 5065.314 kb on + strand, within HSERO_RS22150 at 5065.448 kb on + strand at 5065.448 kb on + strand at 5065.534 kb on - strand at 5065.576 kb on + strand at 5065.579 kb on + strand at 5065.579 kb on - strand at 5065.820 kb on + strand, within HSERO_RS22155 at 5065.825 kb on + strand, within HSERO_RS22155 at 5065.874 kb on - strand, within HSERO_RS22155 at 5065.880 kb on - strand, within HSERO_RS22155 at 5065.936 kb on - strand, within HSERO_RS22155 at 5066.195 kb on + strand at 5066.267 kb on + strand at 5066.309 kb on + strand at 5066.363 kb on - strand at 5066.532 kb on + strand, within HSERO_RS22160 at 5066.540 kb on - strand, within HSERO_RS22160 at 5066.815 kb on - strand, within HSERO_RS22160 at 5067.042 kb on - strand, within HSERO_RS22160
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 5,064,723 + HSERO_RS22145 0.89 +0.1 5,064,723 + HSERO_RS22145 0.89 +0.1 5,064,789 - -0.2 5,065,003 + HSERO_RS22150 0.18 +0.2 5,065,008 + HSERO_RS22150 0.19 +0.0 5,065,115 - HSERO_RS22150 0.38 +0.4 5,065,314 + HSERO_RS22150 0.72 -0.0 5,065,448 + +0.0 5,065,448 + +0.3 5,065,534 - +1.5 5,065,576 + -0.4 5,065,579 + +0.1 5,065,579 - -0.0 5,065,820 + HSERO_RS22155 0.34 +0.2 5,065,825 + HSERO_RS22155 0.35 -0.0 5,065,874 - HSERO_RS22155 0.45 -0.4 5,065,880 - HSERO_RS22155 0.46 +1.4 5,065,936 - HSERO_RS22155 0.57 +0.4 5,066,195 + +0.2 5,066,267 + +0.2 5,066,309 + +0.2 5,066,363 - +1.2 5,066,532 + HSERO_RS22160 0.12 -0.1 5,066,540 - HSERO_RS22160 0.12 +0.4 5,066,815 - HSERO_RS22160 0.21 -0.3 5,067,042 - HSERO_RS22160 0.28 -0.3
Or see this region's nucleotide sequence