Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS19755 and HSERO_RS19760 are separated by 82 nucleotides HSERO_RS19760 and HSERO_RS19765 are separated by 95 nucleotides
HSERO_RS19755: HSERO_RS19755 - betaine-aldehyde dehydrogenase, at 4,530,852 to 4,532,306
_RS19755
HSERO_RS19760: HSERO_RS19760 - alanine racemase, at 4,532,389 to 4,533,495
_RS19760
HSERO_RS19765: HSERO_RS19765 - amino acid permease, at 4,533,591 to 4,535,048
_RS19765
Position (kb)
4532
4533
4534 Strain fitness (log2 ratio)
-2
-1
0
1 at 4531.424 kb on - strand, within HSERO_RS19755 at 4531.509 kb on + strand, within HSERO_RS19755 at 4531.603 kb on - strand, within HSERO_RS19755 at 4531.603 kb on - strand, within HSERO_RS19755 at 4531.609 kb on + strand, within HSERO_RS19755 at 4531.617 kb on - strand, within HSERO_RS19755 at 4531.671 kb on + strand, within HSERO_RS19755 at 4531.701 kb on + strand, within HSERO_RS19755 at 4531.832 kb on + strand, within HSERO_RS19755 at 4532.156 kb on - strand, within HSERO_RS19755 at 4532.269 kb on - strand at 4532.325 kb on - strand at 4532.393 kb on + strand at 4532.417 kb on + strand at 4532.483 kb on + strand at 4532.501 kb on - strand, within HSERO_RS19760 at 4532.550 kb on - strand, within HSERO_RS19760 at 4532.552 kb on - strand, within HSERO_RS19760 at 4532.575 kb on + strand, within HSERO_RS19760 at 4532.625 kb on + strand, within HSERO_RS19760 at 4532.625 kb on + strand, within HSERO_RS19760 at 4532.715 kb on - strand, within HSERO_RS19760 at 4532.725 kb on - strand at 4532.870 kb on - strand, within HSERO_RS19760 at 4532.972 kb on + strand, within HSERO_RS19760 at 4533.184 kb on + strand, within HSERO_RS19760 at 4533.233 kb on - strand, within HSERO_RS19760 at 4533.503 kb on + strand at 4533.505 kb on + strand at 4533.513 kb on - strand at 4533.588 kb on + strand at 4533.588 kb on + strand at 4533.710 kb on + strand at 4533.717 kb on + strand at 4533.875 kb on - strand, within HSERO_RS19765 at 4534.169 kb on + strand, within HSERO_RS19765 at 4534.375 kb on - strand, within HSERO_RS19765 at 4534.472 kb on + strand, within HSERO_RS19765 at 4534.474 kb on - strand, within HSERO_RS19765
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 4,531,424 - HSERO_RS19755 0.39 -0.2 4,531,509 + HSERO_RS19755 0.45 -0.0 4,531,603 - HSERO_RS19755 0.52 -0.8 4,531,603 - HSERO_RS19755 0.52 -0.2 4,531,609 + HSERO_RS19755 0.52 -0.2 4,531,617 - HSERO_RS19755 0.53 +0.2 4,531,671 + HSERO_RS19755 0.56 +0.0 4,531,701 + HSERO_RS19755 0.58 -0.5 4,531,832 + HSERO_RS19755 0.67 +0.1 4,532,156 - HSERO_RS19755 0.90 +1.0 4,532,269 - +0.2 4,532,325 - -0.1 4,532,393 + -0.0 4,532,417 + +1.0 4,532,483 + -0.2 4,532,501 - HSERO_RS19760 0.10 -0.0 4,532,550 - HSERO_RS19760 0.15 -0.2 4,532,552 - HSERO_RS19760 0.15 +0.1 4,532,575 + HSERO_RS19760 0.17 +1.0 4,532,625 + HSERO_RS19760 0.21 -0.2 4,532,625 + HSERO_RS19760 0.21 -0.2 4,532,715 - HSERO_RS19760 0.29 +0.2 4,532,725 - +0.5 4,532,870 - HSERO_RS19760 0.43 -0.4 4,532,972 + HSERO_RS19760 0.53 +0.3 4,533,184 + HSERO_RS19760 0.72 +0.1 4,533,233 - HSERO_RS19760 0.76 +0.1 4,533,503 + +0.2 4,533,505 + -0.2 4,533,513 - -0.1 4,533,588 + +0.2 4,533,588 + +0.4 4,533,710 + +0.2 4,533,717 + +1.0 4,533,875 - HSERO_RS19765 0.19 -0.4 4,534,169 + HSERO_RS19765 0.40 +0.1 4,534,375 - HSERO_RS19765 0.54 -2.4 4,534,472 + HSERO_RS19765 0.60 +0.2 4,534,474 - HSERO_RS19765 0.61 -0.2
Or see this region's nucleotide sequence