Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS19115 and HSERO_RS19120 are separated by 304 nucleotides HSERO_RS19120 and HSERO_RS19125 are separated by 135 nucleotides HSERO_RS19125 and HSERO_RS19130 are separated by 87 nucleotides
HSERO_RS19115: HSERO_RS19115 - D-lactate dehydrogenase, at 4,385,787 to 4,387,202
_RS19115
HSERO_RS19120: HSERO_RS19120 - ATP--cobalamin adenosyltransferase, at 4,387,507 to 4,388,061
_RS19120
HSERO_RS19125: HSERO_RS19125 - 1-deoxy-D-xylulose-5-phosphate synthase, at 4,388,197 to 4,390,062
_RS19125
HSERO_RS19130: HSERO_RS19130 - geranyl transferase, at 4,390,150 to 4,391,097
_RS19130
Position (kb)
4388
4389
4390
4391 Strain fitness (log2 ratio)
-1
0
1 at 4387.206 kb on + strand at 4387.315 kb on - strand at 4387.326 kb on + strand at 4387.342 kb on + strand at 4387.375 kb on - strand at 4387.375 kb on - strand at 4387.379 kb on - strand at 4387.474 kb on + strand at 4387.474 kb on + strand at 4387.482 kb on - strand at 4387.688 kb on - strand, within HSERO_RS19120 at 4387.722 kb on + strand, within HSERO_RS19120 at 4387.734 kb on + strand, within HSERO_RS19120 at 4387.743 kb on + strand, within HSERO_RS19120 at 4387.792 kb on - strand, within HSERO_RS19120 at 4387.944 kb on - strand, within HSERO_RS19120 at 4388.009 kb on + strand at 4388.055 kb on + strand at 4388.162 kb on + strand at 4388.167 kb on + strand at 4388.172 kb on + strand at 4388.172 kb on + strand at 4388.180 kb on - strand at 4388.224 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 4,387,206 + +0.5 4,387,315 - +0.0 4,387,326 + +0.1 4,387,342 + +0.3 4,387,375 - +0.2 4,387,375 - +0.0 4,387,379 - -0.3 4,387,474 + +0.1 4,387,474 + -0.6 4,387,482 - -0.3 4,387,688 - HSERO_RS19120 0.33 +0.1 4,387,722 + HSERO_RS19120 0.39 +0.3 4,387,734 + HSERO_RS19120 0.41 -0.4 4,387,743 + HSERO_RS19120 0.43 +0.2 4,387,792 - HSERO_RS19120 0.51 -0.2 4,387,944 - HSERO_RS19120 0.79 +0.7 4,388,009 + -0.2 4,388,055 + +0.2 4,388,162 + -0.1 4,388,167 + -0.7 4,388,172 + +0.0 4,388,172 + +0.0 4,388,180 - -0.1 4,388,224 - -0.1
Or see this region's nucleotide sequence