Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS17185 and HSERO_RS17190 are separated by 124 nucleotides HSERO_RS17190 and HSERO_RS17195 are separated by 222 nucleotides HSERO_RS17195 and HSERO_RS17200 are separated by 19 nucleotides
HSERO_RS17185: HSERO_RS17185 - 2-isopropylmalate synthase, at 3,935,004 to 3,936,707
_RS17185
HSERO_RS17190: HSERO_RS17190 - hypothetical protein, at 3,936,832 to 3,937,071
_RS17190
HSERO_RS17195: HSERO_RS17195 - chemotaxis protein, at 3,937,294 to 3,939,045
_RS17195
HSERO_RS17200: HSERO_RS17200 - hypothetical protein, at 3,939,065 to 3,940,300
_RS17200
Position (kb)
3937
3938
3939
3940 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3936.362 kb on + strand, within HSERO_RS17185 at 3936.690 kb on - strand at 3936.755 kb on - strand at 3936.840 kb on + strand at 3936.840 kb on + strand at 3936.949 kb on + strand, within HSERO_RS17190 at 3937.001 kb on + strand, within HSERO_RS17190 at 3937.007 kb on - strand, within HSERO_RS17190 at 3937.007 kb on - strand, within HSERO_RS17190 at 3937.040 kb on - strand, within HSERO_RS17190 at 3937.063 kb on - strand at 3937.088 kb on - strand at 3937.177 kb on + strand at 3937.255 kb on + strand at 3937.269 kb on - strand at 3937.324 kb on + strand at 3937.324 kb on + strand at 3937.376 kb on + strand at 3937.521 kb on - strand, within HSERO_RS17195 at 3937.631 kb on - strand, within HSERO_RS17195 at 3937.711 kb on - strand, within HSERO_RS17195 at 3937.716 kb on - strand, within HSERO_RS17195 at 3937.769 kb on + strand, within HSERO_RS17195 at 3937.831 kb on - strand, within HSERO_RS17195 at 3938.003 kb on + strand, within HSERO_RS17195 at 3938.089 kb on - strand, within HSERO_RS17195 at 3938.173 kb on - strand, within HSERO_RS17195 at 3938.175 kb on + strand, within HSERO_RS17195 at 3938.183 kb on + strand, within HSERO_RS17195 at 3938.183 kb on + strand, within HSERO_RS17195 at 3938.183 kb on + strand, within HSERO_RS17195 at 3938.328 kb on + strand, within HSERO_RS17195 at 3938.331 kb on - strand, within HSERO_RS17195 at 3938.465 kb on + strand, within HSERO_RS17195 at 3938.480 kb on + strand, within HSERO_RS17195 at 3938.632 kb on - strand, within HSERO_RS17195 at 3938.662 kb on + strand, within HSERO_RS17195 at 3938.695 kb on - strand, within HSERO_RS17195 at 3938.704 kb on - strand, within HSERO_RS17195 at 3938.759 kb on - strand, within HSERO_RS17195 at 3938.772 kb on + strand, within HSERO_RS17195 at 3938.931 kb on - strand at 3939.134 kb on + strand at 3939.149 kb on + strand at 3939.149 kb on + strand at 3939.149 kb on + strand at 3939.157 kb on - strand at 3939.157 kb on - strand at 3939.157 kb on - strand at 3939.385 kb on - strand, within HSERO_RS17200 at 3939.407 kb on + strand, within HSERO_RS17200 at 3939.494 kb on - strand, within HSERO_RS17200 at 3939.512 kb on + strand, within HSERO_RS17200 at 3939.700 kb on + strand, within HSERO_RS17200 at 3939.725 kb on + strand, within HSERO_RS17200 at 3940.015 kb on - strand, within HSERO_RS17200
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,936,362 + HSERO_RS17185 0.80 -3.3 3,936,690 - +0.1 3,936,755 - -1.4 3,936,840 + -0.1 3,936,840 + +0.4 3,936,949 + HSERO_RS17190 0.49 +0.4 3,937,001 + HSERO_RS17190 0.70 -0.0 3,937,007 - HSERO_RS17190 0.73 +0.2 3,937,007 - HSERO_RS17190 0.73 -0.4 3,937,040 - HSERO_RS17190 0.87 +0.5 3,937,063 - -0.2 3,937,088 - -0.0 3,937,177 + -0.2 3,937,255 + +0.1 3,937,269 - -0.2 3,937,324 + +0.1 3,937,324 + -0.0 3,937,376 + +0.1 3,937,521 - HSERO_RS17195 0.13 -0.3 3,937,631 - HSERO_RS17195 0.19 +0.4 3,937,711 - HSERO_RS17195 0.24 -0.4 3,937,716 - HSERO_RS17195 0.24 +0.5 3,937,769 + HSERO_RS17195 0.27 +0.2 3,937,831 - HSERO_RS17195 0.31 -0.1 3,938,003 + HSERO_RS17195 0.40 +0.9 3,938,089 - HSERO_RS17195 0.45 -0.7 3,938,173 - HSERO_RS17195 0.50 +2.1 3,938,175 + HSERO_RS17195 0.50 +0.6 3,938,183 + HSERO_RS17195 0.51 -0.3 3,938,183 + HSERO_RS17195 0.51 +0.2 3,938,183 + HSERO_RS17195 0.51 -0.1 3,938,328 + HSERO_RS17195 0.59 +0.3 3,938,331 - HSERO_RS17195 0.59 +0.3 3,938,465 + HSERO_RS17195 0.67 +0.0 3,938,480 + HSERO_RS17195 0.68 -0.1 3,938,632 - HSERO_RS17195 0.76 -0.2 3,938,662 + HSERO_RS17195 0.78 +0.2 3,938,695 - HSERO_RS17195 0.80 +0.5 3,938,704 - HSERO_RS17195 0.80 +0.1 3,938,759 - HSERO_RS17195 0.84 +0.0 3,938,772 + HSERO_RS17195 0.84 -0.3 3,938,931 - +0.6 3,939,134 + -0.7 3,939,149 + -0.4 3,939,149 + -0.7 3,939,149 + -0.6 3,939,157 - -0.1 3,939,157 - -0.2 3,939,157 - -0.3 3,939,385 - HSERO_RS17200 0.26 -0.7 3,939,407 + HSERO_RS17200 0.28 -1.4 3,939,494 - HSERO_RS17200 0.35 -0.3 3,939,512 + HSERO_RS17200 0.36 -0.1 3,939,700 + HSERO_RS17200 0.51 -0.1 3,939,725 + HSERO_RS17200 0.53 -1.0 3,940,015 - HSERO_RS17200 0.77 -0.5
Or see this region's nucleotide sequence