Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS16675 and HSERO_RS16680 overlap by 13 nucleotides HSERO_RS16680 and HSERO_RS16685 are separated by 8 nucleotides HSERO_RS16685 and HSERO_RS16690 are separated by 39 nucleotides
HSERO_RS16675: HSERO_RS16675 - hypothetical protein, at 3,817,238 to 3,817,807
_RS16675
HSERO_RS16680: HSERO_RS16680 - GntR family transcriptional regulator, at 3,817,795 to 3,818,565
_RS16680
HSERO_RS16685: HSERO_RS16685 - lactate dehydrogenase, at 3,818,574 to 3,819,647
_RS16685
HSERO_RS16690: HSERO_RS16690 - C4-dicarboxylate ABC transporter permease, at 3,819,687 to 3,821,183
_RS16690
Position (kb)
3818
3819
3820 Strain fitness (log2 ratio)
-1
0
1
2 at 3817.656 kb on - strand, within HSERO_RS16675 at 3818.273 kb on + strand, within HSERO_RS16680 at 3818.318 kb on - strand, within HSERO_RS16680 at 3818.577 kb on + strand at 3818.649 kb on - strand at 3818.661 kb on - strand at 3818.847 kb on - strand, within HSERO_RS16685 at 3818.898 kb on + strand, within HSERO_RS16685 at 3819.122 kb on + strand, within HSERO_RS16685 at 3819.242 kb on - strand, within HSERO_RS16685 at 3819.428 kb on + strand, within HSERO_RS16685 at 3819.600 kb on - strand at 3819.661 kb on + strand at 3819.684 kb on + strand at 3819.684 kb on + strand at 3819.704 kb on - strand at 3819.861 kb on - strand, within HSERO_RS16690 at 3819.875 kb on - strand, within HSERO_RS16690 at 3819.938 kb on - strand, within HSERO_RS16690 at 3819.955 kb on + strand, within HSERO_RS16690 at 3819.960 kb on + strand, within HSERO_RS16690 at 3819.968 kb on - strand, within HSERO_RS16690 at 3820.226 kb on - strand, within HSERO_RS16690 at 3820.535 kb on + strand, within HSERO_RS16690
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,817,656 - HSERO_RS16675 0.73 +0.1 3,818,273 + HSERO_RS16680 0.62 +0.0 3,818,318 - HSERO_RS16680 0.68 +0.2 3,818,577 + +0.1 3,818,649 - -0.6 3,818,661 - +0.3 3,818,847 - HSERO_RS16685 0.25 +0.2 3,818,898 + HSERO_RS16685 0.30 +0.3 3,819,122 + HSERO_RS16685 0.51 +0.2 3,819,242 - HSERO_RS16685 0.62 +0.7 3,819,428 + HSERO_RS16685 0.80 +2.4 3,819,600 - +0.6 3,819,661 + -0.1 3,819,684 + -0.4 3,819,684 + -0.5 3,819,704 - -0.2 3,819,861 - HSERO_RS16690 0.12 -0.1 3,819,875 - HSERO_RS16690 0.13 +0.4 3,819,938 - HSERO_RS16690 0.17 -0.5 3,819,955 + HSERO_RS16690 0.18 +0.1 3,819,960 + HSERO_RS16690 0.18 -0.2 3,819,968 - HSERO_RS16690 0.19 +0.7 3,820,226 - HSERO_RS16690 0.36 -0.2 3,820,535 + HSERO_RS16690 0.57 -0.0
Or see this region's nucleotide sequence