Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS15770

Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS15760 and HSERO_RS15765 are separated by 54 nucleotidesHSERO_RS15765 and HSERO_RS15770 are separated by 84 nucleotidesHSERO_RS15770 and HSERO_RS15775 are separated by 16 nucleotidesHSERO_RS15775 and HSERO_RS15780 are separated by 304 nucleotides HSERO_RS15760: HSERO_RS15760 - heme biosynthesis protein HemY, at 3,598,686 to 3,599,009 _RS15760 HSERO_RS15765: HSERO_RS15765 - FeS cluster assembly scaffold IscU, at 3,599,064 to 3,599,462 _RS15765 HSERO_RS15770: HSERO_RS15770 - cysteine desulfurase, at 3,599,547 to 3,600,800 _RS15770 HSERO_RS15775: HSERO_RS15775 - DNA-binding protein, at 3,600,817 to 3,601,311 _RS15775 HSERO_RS15780: HSERO_RS15780 - GntR family transcriptional regulator, at 3,601,616 to 3,602,383 _RS15780 Position (kb) 3599 3600 3601Strain fitness (log2 ratio) -1 0 1at 3598.614 kb on - strandat 3599.086 kb on - strandat 3600.854 kb on - strandat 3600.973 kb on - strand, within HSERO_RS15775at 3600.988 kb on - strandat 3601.337 kb on - strandat 3601.412 kb on + strandat 3601.506 kb on - strandat 3601.532 kb on - strandat 3601.576 kb on + strandat 3601.622 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
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3,598,614 - -0.3
3,599,086 - -0.9
3,600,854 - +0.1
3,600,973 - HSERO_RS15775 0.32 +1.0
3,600,988 - +1.4
3,601,337 - +0.1
3,601,412 + -0.3
3,601,506 - -0.2
3,601,532 - +1.1
3,601,576 + +0.8
3,601,622 - -0.3

Or see this region's nucleotide sequence