Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS15760 and HSERO_RS15765 are separated by 54 nucleotides HSERO_RS15765 and HSERO_RS15770 are separated by 84 nucleotides HSERO_RS15770 and HSERO_RS15775 are separated by 16 nucleotides HSERO_RS15775 and HSERO_RS15780 are separated by 304 nucleotides
HSERO_RS15760: HSERO_RS15760 - heme biosynthesis protein HemY, at 3,598,686 to 3,599,009
_RS15760
HSERO_RS15765: HSERO_RS15765 - FeS cluster assembly scaffold IscU, at 3,599,064 to 3,599,462
_RS15765
HSERO_RS15770: HSERO_RS15770 - cysteine desulfurase, at 3,599,547 to 3,600,800
_RS15770
HSERO_RS15775: HSERO_RS15775 - DNA-binding protein, at 3,600,817 to 3,601,311
_RS15775
HSERO_RS15780: HSERO_RS15780 - GntR family transcriptional regulator, at 3,601,616 to 3,602,383
_RS15780
Position (kb)
3599
3600
3601 Strain fitness (log2 ratio)
-1
0
1 at 3598.614 kb on - strand at 3599.086 kb on - strand at 3600.854 kb on - strand at 3600.973 kb on - strand, within HSERO_RS15775 at 3600.988 kb on - strand at 3601.337 kb on - strand at 3601.412 kb on + strand at 3601.506 kb on - strand at 3601.532 kb on - strand at 3601.576 kb on + strand at 3601.622 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,598,614 - -0.3 3,599,086 - -0.9 3,600,854 - +0.1 3,600,973 - HSERO_RS15775 0.32 +1.0 3,600,988 - +1.4 3,601,337 - +0.1 3,601,412 + -0.3 3,601,506 - -0.2 3,601,532 - +1.1 3,601,576 + +0.8 3,601,622 - -0.3
Or see this region's nucleotide sequence