Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14370 and HSERO_RS14375 are separated by 4 nucleotides HSERO_RS14375 and HSERO_RS14380 are separated by 9 nucleotides HSERO_RS14380 and HSERO_RS14385 are separated by 33 nucleotides HSERO_RS14385 and HSERO_RS14390 are separated by 391 nucleotides
HSERO_RS14370: HSERO_RS14370 - hypothetical protein, at 3,254,587 to 3,255,345
_RS14370
HSERO_RS14375: HSERO_RS14375 - IscN protein, at 3,255,350 to 3,255,724
_RS14375
HSERO_RS14380: HSERO_RS14380 - ferredoxin, at 3,255,734 to 3,255,952
_RS14380
HSERO_RS14385: HSERO_RS14385 - FeMo cofactor biosynthesis protein NifB, at 3,255,986 to 3,257,563
_RS14385
HSERO_RS14390: HSERO_RS14390 - ATPase AAA, at 3,257,955 to 3,259,574
_RS14390
Position (kb)
3255
3256
3257
3258 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3255.170 kb on + strand, within HSERO_RS14370 at 3255.301 kb on + strand at 3255.356 kb on - strand at 3255.356 kb on - strand at 3255.436 kb on + strand, within HSERO_RS14375 at 3255.732 kb on + strand at 3255.732 kb on + strand at 3255.732 kb on + strand at 3255.732 kb on + strand at 3256.012 kb on - strand at 3256.017 kb on - strand at 3256.046 kb on + strand at 3256.257 kb on - strand at 3256.291 kb on - strand, within HSERO_RS14385 at 3256.291 kb on - strand, within HSERO_RS14385 at 3256.321 kb on - strand, within HSERO_RS14385 at 3256.423 kb on - strand, within HSERO_RS14385 at 3256.437 kb on - strand, within HSERO_RS14385 at 3256.532 kb on + strand, within HSERO_RS14385 at 3256.540 kb on - strand, within HSERO_RS14385 at 3256.743 kb on + strand, within HSERO_RS14385 at 3257.030 kb on - strand, within HSERO_RS14385 at 3257.040 kb on + strand, within HSERO_RS14385 at 3257.094 kb on - strand, within HSERO_RS14385 at 3257.177 kb on - strand, within HSERO_RS14385 at 3257.368 kb on + strand at 3257.434 kb on - strand at 3257.491 kb on - strand at 3257.594 kb on + strand at 3257.682 kb on - strand at 3257.734 kb on + strand at 3257.792 kb on - strand at 3257.804 kb on - strand at 3257.813 kb on + strand at 3258.015 kb on + strand at 3258.047 kb on - strand at 3258.093 kb on + strand at 3258.364 kb on - strand, within HSERO_RS14390 at 3258.365 kb on - strand, within HSERO_RS14390 at 3258.446 kb on - strand, within HSERO_RS14390 at 3258.504 kb on - strand, within HSERO_RS14390 at 3258.504 kb on - strand, within HSERO_RS14390 at 3258.516 kb on + strand, within HSERO_RS14390 at 3258.543 kb on - strand, within HSERO_RS14390
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,255,170 + HSERO_RS14370 0.77 -0.7 3,255,301 + -0.9 3,255,356 - +0.4 3,255,356 - -0.4 3,255,436 + HSERO_RS14375 0.23 -0.7 3,255,732 + -1.2 3,255,732 + -1.2 3,255,732 + -1.6 3,255,732 + -1.0 3,256,012 - -0.2 3,256,017 - -1.0 3,256,046 + -1.6 3,256,257 - -2.2 3,256,291 - HSERO_RS14385 0.19 -4.4 3,256,291 - HSERO_RS14385 0.19 -4.1 3,256,321 - HSERO_RS14385 0.21 -3.4 3,256,423 - HSERO_RS14385 0.28 -4.3 3,256,437 - HSERO_RS14385 0.29 -3.4 3,256,532 + HSERO_RS14385 0.35 -1.6 3,256,540 - HSERO_RS14385 0.35 -5.4 3,256,743 + HSERO_RS14385 0.48 -1.2 3,257,030 - HSERO_RS14385 0.66 -3.9 3,257,040 + HSERO_RS14385 0.67 -4.4 3,257,094 - HSERO_RS14385 0.70 -1.7 3,257,177 - HSERO_RS14385 0.75 -4.2 3,257,368 + -1.7 3,257,434 - -4.1 3,257,491 - -4.4 3,257,594 + -3.3 3,257,682 - -4.2 3,257,734 + -0.6 3,257,792 - -0.5 3,257,804 - +0.4 3,257,813 + +0.1 3,258,015 + -2.3 3,258,047 - -1.0 3,258,093 + -2.8 3,258,364 - HSERO_RS14390 0.25 -4.2 3,258,365 - HSERO_RS14390 0.25 -3.9 3,258,446 - HSERO_RS14390 0.30 -3.9 3,258,504 - HSERO_RS14390 0.34 -3.3 3,258,504 - HSERO_RS14390 0.34 -3.6 3,258,516 + HSERO_RS14390 0.35 -1.9 3,258,543 - HSERO_RS14390 0.36 -3.2
Or see this region's nucleotide sequence