Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14325 and HSERO_RS14330 are separated by 35 nucleotides HSERO_RS14330 and HSERO_RS14335 are separated by 73 nucleotides HSERO_RS14335 and HSERO_RS14340 are separated by 30 nucleotides HSERO_RS14340 and HSERO_RS14345 are separated by 9 nucleotides HSERO_RS14345 and HSERO_RS14350 are separated by 44 nucleotides
HSERO_RS14325: HSERO_RS14325 - hypothetical protein, at 3,249,525 to 3,250,376
_RS14325
HSERO_RS14330: HSERO_RS14330 - ferredoxin, at 3,250,412 to 3,250,717
_RS14330
HSERO_RS14335: HSERO_RS14335 - aminotransferase DegT, at 3,250,791 to 3,251,939
_RS14335
HSERO_RS14340: HSERO_RS14340 - hypothetical protein, at 3,251,970 to 3,252,230
_RS14340
HSERO_RS14345: HSERO_RS14345 - protein fixU, at 3,252,240 to 3,252,440
_RS14345
HSERO_RS14350: HSERO_RS14350 - cysteine desulfurase, at 3,252,485 to 3,253,624
_RS14350
Position (kb) 3250
3251
3252 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3249.906 kb on + strand, within HSERO_RS14325 at 3249.968 kb on + strand, within HSERO_RS14325 at 3249.983 kb on - strand, within HSERO_RS14325 at 3250.209 kb on + strand, within HSERO_RS14325 at 3250.270 kb on + strand at 3250.318 kb on + strand at 3250.397 kb on + strand at 3250.518 kb on + strand, within HSERO_RS14330 at 3250.585 kb on - strand at 3250.657 kb on + strand, within HSERO_RS14330 at 3250.718 kb on + strand at 3250.718 kb on + strand at 3250.721 kb on - strand at 3250.867 kb on + strand at 3250.867 kb on + strand at 3250.887 kb on + strand at 3251.036 kb on + strand, within HSERO_RS14335 at 3251.058 kb on + strand, within HSERO_RS14335 at 3251.372 kb on + strand, within HSERO_RS14335 at 3251.374 kb on + strand, within HSERO_RS14335 at 3251.521 kb on - strand, within HSERO_RS14335 at 3251.523 kb on + strand, within HSERO_RS14335 at 3251.663 kb on + strand, within HSERO_RS14335 at 3251.855 kb on - strand at 3251.904 kb on - strand at 3251.958 kb on - strand at 3252.066 kb on + strand, within HSERO_RS14340 at 3252.163 kb on + strand, within HSERO_RS14340 at 3252.176 kb on - strand, within HSERO_RS14340 at 3252.443 kb on + strand at 3252.569 kb on + strand at 3252.728 kb on - strand, within HSERO_RS14350 at 3252.834 kb on - strand, within HSERO_RS14350 at 3252.834 kb on - strand, within HSERO_RS14350 at 3252.869 kb on + strand, within HSERO_RS14350
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,249,906 + HSERO_RS14325 0.45 +0.3 3,249,968 + HSERO_RS14325 0.52 +0.3 3,249,983 - HSERO_RS14325 0.54 +0.8 3,250,209 + HSERO_RS14325 0.80 +0.4 3,250,270 + +0.0 3,250,318 + +0.8 3,250,397 + -3.0 3,250,518 + HSERO_RS14330 0.35 -0.1 3,250,585 - -1.6 3,250,657 + HSERO_RS14330 0.80 -0.1 3,250,718 + -0.7 3,250,718 + +0.2 3,250,721 - -0.1 3,250,867 + -1.4 3,250,867 + -2.3 3,250,887 + -2.0 3,251,036 + HSERO_RS14335 0.21 -1.8 3,251,058 + HSERO_RS14335 0.23 -2.6 3,251,372 + HSERO_RS14335 0.51 -0.9 3,251,374 + HSERO_RS14335 0.51 -1.6 3,251,521 - HSERO_RS14335 0.64 -1.4 3,251,523 + HSERO_RS14335 0.64 -1.5 3,251,663 + HSERO_RS14335 0.76 -0.9 3,251,855 - -1.1 3,251,904 - -1.7 3,251,958 - -0.6 3,252,066 + HSERO_RS14340 0.37 -1.2 3,252,163 + HSERO_RS14340 0.74 -1.1 3,252,176 - HSERO_RS14340 0.79 +0.4 3,252,443 + -0.4 3,252,569 + -1.5 3,252,728 - HSERO_RS14350 0.21 -0.2 3,252,834 - HSERO_RS14350 0.31 +0.1 3,252,834 - HSERO_RS14350 0.31 +0.8 3,252,869 + HSERO_RS14350 0.34 -1.1
Or see this region's nucleotide sequence