Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14285 and HSERO_RS14290 are separated by 88 nucleotides HSERO_RS14290 and HSERO_RS14295 are separated by 105 nucleotides
HSERO_RS14285: HSERO_RS14285 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 3,241,552 to 3,242,931
_RS14285
HSERO_RS14290: HSERO_RS14290 - nitrogenase molybdenum-iron protein beta chain, at 3,243,020 to 3,244,579
_RS14290
HSERO_RS14295: HSERO_RS14295 - nitrogenase molybdenum-iron protein alpha chain, at 3,244,685 to 3,246,139
_RS14295
Position (kb)
3243
3244
3245 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3242.437 kb on - strand, within HSERO_RS14285 at 3242.550 kb on - strand, within HSERO_RS14285 at 3242.648 kb on - strand, within HSERO_RS14285 at 3242.671 kb on + strand, within HSERO_RS14285 at 3242.701 kb on - strand at 3242.861 kb on + strand at 3242.947 kb on - strand at 3243.129 kb on + strand at 3243.399 kb on - strand at 3243.418 kb on - strand, within HSERO_RS14290 at 3243.543 kb on - strand, within HSERO_RS14290 at 3243.697 kb on + strand, within HSERO_RS14290 at 3243.808 kb on + strand, within HSERO_RS14290 at 3243.833 kb on - strand, within HSERO_RS14290 at 3243.867 kb on - strand, within HSERO_RS14290 at 3243.951 kb on + strand, within HSERO_RS14290 at 3244.024 kb on - strand, within HSERO_RS14290 at 3244.097 kb on - strand, within HSERO_RS14290 at 3244.242 kb on + strand, within HSERO_RS14290 at 3244.289 kb on - strand, within HSERO_RS14290 at 3244.374 kb on + strand, within HSERO_RS14290 at 3244.374 kb on + strand, within HSERO_RS14290 at 3244.442 kb on - strand at 3244.462 kb on - strand at 3244.625 kb on - strand at 3244.660 kb on - strand at 3244.709 kb on - strand at 3244.727 kb on + strand at 3244.734 kb on + strand at 3244.786 kb on + strand at 3244.934 kb on - strand, within HSERO_RS14295 at 3245.100 kb on + strand, within HSERO_RS14295 at 3245.108 kb on - strand, within HSERO_RS14295 at 3245.126 kb on + strand, within HSERO_RS14295 at 3245.133 kb on + strand, within HSERO_RS14295 at 3245.133 kb on + strand, within HSERO_RS14295 at 3245.286 kb on + strand, within HSERO_RS14295 at 3245.294 kb on - strand, within HSERO_RS14295
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,242,437 - HSERO_RS14285 0.64 -2.3 3,242,550 - HSERO_RS14285 0.72 -3.9 3,242,648 - HSERO_RS14285 0.79 -4.4 3,242,671 + HSERO_RS14285 0.81 -4.6 3,242,701 - -3.2 3,242,861 + -5.0 3,242,947 - -0.0 3,243,129 + -1.7 3,243,399 - -3.0 3,243,418 - HSERO_RS14290 0.26 -4.2 3,243,543 - HSERO_RS14290 0.34 -4.8 3,243,697 + HSERO_RS14290 0.43 -3.8 3,243,808 + HSERO_RS14290 0.51 -3.6 3,243,833 - HSERO_RS14290 0.52 -3.4 3,243,867 - HSERO_RS14290 0.54 -4.7 3,243,951 + HSERO_RS14290 0.60 -4.8 3,244,024 - HSERO_RS14290 0.64 -4.7 3,244,097 - HSERO_RS14290 0.69 -3.0 3,244,242 + HSERO_RS14290 0.78 -2.1 3,244,289 - HSERO_RS14290 0.81 -4.4 3,244,374 + HSERO_RS14290 0.87 -3.3 3,244,374 + HSERO_RS14290 0.87 -2.9 3,244,442 - -3.3 3,244,462 - -4.2 3,244,625 - -0.7 3,244,660 - -0.3 3,244,709 - -3.2 3,244,727 + -2.9 3,244,734 + -2.6 3,244,786 + -2.7 3,244,934 - HSERO_RS14295 0.17 -2.8 3,245,100 + HSERO_RS14295 0.29 -3.4 3,245,108 - HSERO_RS14295 0.29 -2.4 3,245,126 + HSERO_RS14295 0.30 -5.2 3,245,133 + HSERO_RS14295 0.31 -3.6 3,245,133 + HSERO_RS14295 0.31 -5.3 3,245,286 + HSERO_RS14295 0.41 -4.4 3,245,294 - HSERO_RS14295 0.42 -4.4
Or see this region's nucleotide sequence