Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14280 and HSERO_RS14285 are separated by 4 nucleotides HSERO_RS14285 and HSERO_RS14290 are separated by 88 nucleotides
HSERO_RS14280: HSERO_RS14280 - nitrogenase molybdenum-cofactor synthesis protein NifN protein, at 3,240,159 to 3,241,547
_RS14280
HSERO_RS14285: HSERO_RS14285 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 3,241,552 to 3,242,931
_RS14285
HSERO_RS14290: HSERO_RS14290 - nitrogenase molybdenum-iron protein beta chain, at 3,243,020 to 3,244,579
_RS14290
Position (kb)
3241
3242
3243 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3240.600 kb on + strand, within HSERO_RS14280 at 3240.745 kb on + strand, within HSERO_RS14280 at 3240.756 kb on + strand, within HSERO_RS14280 at 3240.872 kb on + strand, within HSERO_RS14280 at 3241.155 kb on + strand, within HSERO_RS14280 at 3241.163 kb on - strand, within HSERO_RS14280 at 3241.163 kb on - strand, within HSERO_RS14280 at 3241.168 kb on - strand, within HSERO_RS14280 at 3241.207 kb on - strand, within HSERO_RS14280 at 3241.231 kb on + strand, within HSERO_RS14280 at 3241.265 kb on + strand, within HSERO_RS14280 at 3241.276 kb on + strand, within HSERO_RS14280 at 3241.412 kb on + strand at 3241.414 kb on + strand at 3241.420 kb on - strand at 3241.467 kb on + strand at 3241.475 kb on - strand at 3241.475 kb on - strand at 3241.475 kb on - strand at 3241.475 kb on - strand at 3241.475 kb on - strand at 3241.475 kb on - strand at 3241.600 kb on - strand at 3241.890 kb on + strand, within HSERO_RS14285 at 3241.988 kb on + strand, within HSERO_RS14285 at 3241.988 kb on + strand, within HSERO_RS14285 at 3242.437 kb on - strand, within HSERO_RS14285 at 3242.550 kb on - strand, within HSERO_RS14285 at 3242.648 kb on - strand, within HSERO_RS14285 at 3242.671 kb on + strand, within HSERO_RS14285 at 3242.701 kb on - strand at 3242.861 kb on + strand at 3242.947 kb on - strand at 3243.129 kb on + strand at 3243.399 kb on - strand at 3243.418 kb on - strand, within HSERO_RS14290 at 3243.543 kb on - strand, within HSERO_RS14290 at 3243.697 kb on + strand, within HSERO_RS14290 at 3243.808 kb on + strand, within HSERO_RS14290 at 3243.833 kb on - strand, within HSERO_RS14290 at 3243.867 kb on - strand, within HSERO_RS14290
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,240,600 + HSERO_RS14280 0.32 -5.1 3,240,745 + HSERO_RS14280 0.42 -4.1 3,240,756 + HSERO_RS14280 0.43 -2.4 3,240,872 + HSERO_RS14280 0.51 -0.6 3,241,155 + HSERO_RS14280 0.72 -3.1 3,241,163 - HSERO_RS14280 0.72 -4.3 3,241,163 - HSERO_RS14280 0.72 -2.6 3,241,168 - HSERO_RS14280 0.73 -4.3 3,241,207 - HSERO_RS14280 0.75 -3.7 3,241,231 + HSERO_RS14280 0.77 -3.3 3,241,265 + HSERO_RS14280 0.80 -2.6 3,241,276 + HSERO_RS14280 0.80 -4.0 3,241,412 + -4.8 3,241,414 + -1.4 3,241,420 - -4.6 3,241,467 + -2.5 3,241,475 - -4.1 3,241,475 - -3.9 3,241,475 - -5.2 3,241,475 - -4.2 3,241,475 - -3.3 3,241,475 - -4.4 3,241,600 - -4.6 3,241,890 + HSERO_RS14285 0.24 -4.8 3,241,988 + HSERO_RS14285 0.32 -3.7 3,241,988 + HSERO_RS14285 0.32 -4.6 3,242,437 - HSERO_RS14285 0.64 -2.3 3,242,550 - HSERO_RS14285 0.72 -3.9 3,242,648 - HSERO_RS14285 0.79 -4.4 3,242,671 + HSERO_RS14285 0.81 -4.6 3,242,701 - -3.2 3,242,861 + -5.0 3,242,947 - -0.0 3,243,129 + -1.7 3,243,399 - -3.0 3,243,418 - HSERO_RS14290 0.26 -4.2 3,243,543 - HSERO_RS14290 0.34 -4.8 3,243,697 + HSERO_RS14290 0.43 -3.8 3,243,808 + HSERO_RS14290 0.51 -3.6 3,243,833 - HSERO_RS14290 0.52 -3.4 3,243,867 - HSERO_RS14290 0.54 -4.7
Or see this region's nucleotide sequence