Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14225 and HSERO_RS14230 are separated by 20 nucleotides HSERO_RS14230 and HSERO_RS14235 overlap by 4 nucleotides HSERO_RS14235 and HSERO_RS14240 are separated by 81 nucleotides
HSERO_RS14225: HSERO_RS14225 - protein fixB, at 3,232,630 to 3,233,724
_RS14225
HSERO_RS14230: HSERO_RS14230 - protein fixC, at 3,233,745 to 3,235,043
_RS14230
HSERO_RS14235: HSERO_RS14235 - ferredoxin, at 3,235,040 to 3,235,333
_RS14235
HSERO_RS14240: HSERO_RS14240 - molybdenum ABC transporter ATP-binding protein, at 3,235,415 to 3,236,533
_RS14240
Position (kb)
3233
3234
3235
3236 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3232.760 kb on + strand, within HSERO_RS14225 at 3232.768 kb on - strand, within HSERO_RS14225 at 3232.825 kb on + strand, within HSERO_RS14225 at 3232.910 kb on + strand, within HSERO_RS14225 at 3232.918 kb on - strand, within HSERO_RS14225 at 3233.051 kb on + strand, within HSERO_RS14225 at 3233.548 kb on + strand, within HSERO_RS14225 at 3233.679 kb on - strand at 3233.956 kb on + strand, within HSERO_RS14230 at 3233.974 kb on - strand, within HSERO_RS14230 at 3234.240 kb on + strand, within HSERO_RS14230 at 3234.417 kb on + strand, within HSERO_RS14230 at 3234.422 kb on + strand, within HSERO_RS14230 at 3234.699 kb on + strand, within HSERO_RS14230 at 3234.808 kb on - strand, within HSERO_RS14230 at 3234.894 kb on - strand, within HSERO_RS14230 at 3234.894 kb on - strand, within HSERO_RS14230 at 3234.914 kb on + strand at 3234.928 kb on - strand at 3235.047 kb on + strand at 3235.152 kb on - strand, within HSERO_RS14235 at 3235.223 kb on + strand, within HSERO_RS14235 at 3235.421 kb on - strand at 3235.439 kb on - strand at 3235.548 kb on + strand, within HSERO_RS14240 at 3235.556 kb on - strand, within HSERO_RS14240 at 3235.618 kb on - strand, within HSERO_RS14240 at 3235.662 kb on + strand, within HSERO_RS14240 at 3235.860 kb on - strand, within HSERO_RS14240 at 3235.956 kb on + strand, within HSERO_RS14240
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,232,760 + HSERO_RS14225 0.12 -4.3 3,232,768 - HSERO_RS14225 0.13 -3.1 3,232,825 + HSERO_RS14225 0.18 -3.8 3,232,910 + HSERO_RS14225 0.26 -4.0 3,232,918 - HSERO_RS14225 0.26 -4.7 3,233,051 + HSERO_RS14225 0.38 -5.6 3,233,548 + HSERO_RS14225 0.84 -2.7 3,233,679 - -2.6 3,233,956 + HSERO_RS14230 0.16 -4.6 3,233,974 - HSERO_RS14230 0.18 -4.4 3,234,240 + HSERO_RS14230 0.38 -4.2 3,234,417 + HSERO_RS14230 0.52 -3.2 3,234,422 + HSERO_RS14230 0.52 -3.1 3,234,699 + HSERO_RS14230 0.73 -3.9 3,234,808 - HSERO_RS14230 0.82 -2.4 3,234,894 - HSERO_RS14230 0.88 -3.3 3,234,894 - HSERO_RS14230 0.88 -2.6 3,234,914 + -3.6 3,234,928 - -3.9 3,235,047 + -4.2 3,235,152 - HSERO_RS14235 0.38 -3.9 3,235,223 + HSERO_RS14235 0.62 -2.1 3,235,421 - +0.8 3,235,439 - -0.7 3,235,548 + HSERO_RS14240 0.12 -0.0 3,235,556 - HSERO_RS14240 0.13 +0.1 3,235,618 - HSERO_RS14240 0.18 -0.3 3,235,662 + HSERO_RS14240 0.22 +0.1 3,235,860 - HSERO_RS14240 0.40 -0.2 3,235,956 + HSERO_RS14240 0.48 -0.4
Or see this region's nucleotide sequence