Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS14220

Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS14210 and HSERO_RS14215 are separated by 67 nucleotidesHSERO_RS14215 and HSERO_RS14220 are separated by 22 nucleotidesHSERO_RS14220 and HSERO_RS14225 are separated by 31 nucleotides HSERO_RS14210: HSERO_RS14210 - homocitrate synthase, at 3,230,203 to 3,231,333 _RS14210 HSERO_RS14215: HSERO_RS14215 - nitrogen fixation protein NifW, at 3,231,401 to 3,231,730 _RS14215 HSERO_RS14220: HSERO_RS14220 - protein fixA, at 3,231,753 to 3,232,598 _RS14220 HSERO_RS14225: HSERO_RS14225 - protein fixB, at 3,232,630 to 3,233,724 _RS14225 Position (kb) 3231 3232 3233Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3230.804 kb on - strand, within HSERO_RS14210at 3230.836 kb on + strand, within HSERO_RS14210at 3230.841 kb on + strand, within HSERO_RS14210at 3230.849 kb on - strand, within HSERO_RS14210at 3230.908 kb on + strand, within HSERO_RS14210at 3230.940 kb on + strand, within HSERO_RS14210at 3230.940 kb on + strand, within HSERO_RS14210at 3231.097 kb on + strand, within HSERO_RS14210at 3231.099 kb on + strand, within HSERO_RS14210at 3231.519 kb on + strand, within HSERO_RS14215at 3231.569 kb on + strand, within HSERO_RS14215at 3231.651 kb on - strand, within HSERO_RS14215at 3231.724 kb on + strandat 3231.724 kb on + strandat 3231.932 kb on - strand, within HSERO_RS14220at 3231.947 kb on + strand, within HSERO_RS14220at 3231.960 kb on - strand, within HSERO_RS14220at 3232.045 kb on - strand, within HSERO_RS14220at 3232.055 kb on + strand, within HSERO_RS14220at 3232.137 kb on + strand, within HSERO_RS14220at 3232.383 kb on - strand, within HSERO_RS14220at 3232.409 kb on + strand, within HSERO_RS14220at 3232.473 kb on - strand, within HSERO_RS14220at 3232.473 kb on - strand, within HSERO_RS14220at 3232.552 kb on + strandat 3232.611 kb on - strandat 3232.695 kb on + strandat 3232.760 kb on + strand, within HSERO_RS14225at 3232.768 kb on - strand, within HSERO_RS14225at 3232.825 kb on + strand, within HSERO_RS14225at 3232.910 kb on + strand, within HSERO_RS14225at 3232.918 kb on - strand, within HSERO_RS14225at 3233.051 kb on + strand, within HSERO_RS14225at 3233.548 kb on + strand, within HSERO_RS14225

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
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3,230,804 - HSERO_RS14210 0.53 -4.2
3,230,836 + HSERO_RS14210 0.56 -5.1
3,230,841 + HSERO_RS14210 0.56 -2.2
3,230,849 - HSERO_RS14210 0.57 -2.9
3,230,908 + HSERO_RS14210 0.62 -3.3
3,230,940 + HSERO_RS14210 0.65 -3.2
3,230,940 + HSERO_RS14210 0.65 -1.3
3,231,097 + HSERO_RS14210 0.79 -4.2
3,231,099 + HSERO_RS14210 0.79 -3.5
3,231,519 + HSERO_RS14215 0.36 -3.3
3,231,569 + HSERO_RS14215 0.51 -3.9
3,231,651 - HSERO_RS14215 0.76 -2.0
3,231,724 + -0.8
3,231,724 + +0.2
3,231,932 - HSERO_RS14220 0.21 -3.2
3,231,947 + HSERO_RS14220 0.23 -4.4
3,231,960 - HSERO_RS14220 0.24 -3.7
3,232,045 - HSERO_RS14220 0.35 -5.1
3,232,055 + HSERO_RS14220 0.36 -3.0
3,232,137 + HSERO_RS14220 0.45 +1.6
3,232,383 - HSERO_RS14220 0.74 -3.6
3,232,409 + HSERO_RS14220 0.78 -3.7
3,232,473 - HSERO_RS14220 0.85 -4.5
3,232,473 - HSERO_RS14220 0.85 -5.2
3,232,552 + -2.6
3,232,611 - -3.2
3,232,695 + -3.9
3,232,760 + HSERO_RS14225 0.12 -4.3
3,232,768 - HSERO_RS14225 0.13 -3.1
3,232,825 + HSERO_RS14225 0.18 -3.8
3,232,910 + HSERO_RS14225 0.26 -4.0
3,232,918 - HSERO_RS14225 0.26 -4.7
3,233,051 + HSERO_RS14225 0.38 -5.6
3,233,548 + HSERO_RS14225 0.84 -2.7

Or see this region's nucleotide sequence