Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14210 and HSERO_RS14215 are separated by 67 nucleotides HSERO_RS14215 and HSERO_RS14220 are separated by 22 nucleotides HSERO_RS14220 and HSERO_RS14225 are separated by 31 nucleotides
HSERO_RS14210: HSERO_RS14210 - homocitrate synthase, at 3,230,203 to 3,231,333
_RS14210
HSERO_RS14215: HSERO_RS14215 - nitrogen fixation protein NifW, at 3,231,401 to 3,231,730
_RS14215
HSERO_RS14220: HSERO_RS14220 - protein fixA, at 3,231,753 to 3,232,598
_RS14220
HSERO_RS14225: HSERO_RS14225 - protein fixB, at 3,232,630 to 3,233,724
_RS14225
Position (kb)
3231
3232
3233 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3230.804 kb on - strand, within HSERO_RS14210 at 3230.836 kb on + strand, within HSERO_RS14210 at 3230.841 kb on + strand, within HSERO_RS14210 at 3230.849 kb on - strand, within HSERO_RS14210 at 3230.908 kb on + strand, within HSERO_RS14210 at 3230.940 kb on + strand, within HSERO_RS14210 at 3230.940 kb on + strand, within HSERO_RS14210 at 3231.097 kb on + strand, within HSERO_RS14210 at 3231.099 kb on + strand, within HSERO_RS14210 at 3231.519 kb on + strand, within HSERO_RS14215 at 3231.569 kb on + strand, within HSERO_RS14215 at 3231.651 kb on - strand, within HSERO_RS14215 at 3231.724 kb on + strand at 3231.724 kb on + strand at 3231.932 kb on - strand, within HSERO_RS14220 at 3231.947 kb on + strand, within HSERO_RS14220 at 3231.960 kb on - strand, within HSERO_RS14220 at 3232.045 kb on - strand, within HSERO_RS14220 at 3232.055 kb on + strand, within HSERO_RS14220 at 3232.137 kb on + strand, within HSERO_RS14220 at 3232.383 kb on - strand, within HSERO_RS14220 at 3232.409 kb on + strand, within HSERO_RS14220 at 3232.473 kb on - strand, within HSERO_RS14220 at 3232.473 kb on - strand, within HSERO_RS14220 at 3232.552 kb on + strand at 3232.611 kb on - strand at 3232.695 kb on + strand at 3232.760 kb on + strand, within HSERO_RS14225 at 3232.768 kb on - strand, within HSERO_RS14225 at 3232.825 kb on + strand, within HSERO_RS14225 at 3232.910 kb on + strand, within HSERO_RS14225 at 3232.918 kb on - strand, within HSERO_RS14225 at 3233.051 kb on + strand, within HSERO_RS14225 at 3233.548 kb on + strand, within HSERO_RS14225
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,230,804 - HSERO_RS14210 0.53 -4.2 3,230,836 + HSERO_RS14210 0.56 -5.1 3,230,841 + HSERO_RS14210 0.56 -2.2 3,230,849 - HSERO_RS14210 0.57 -2.9 3,230,908 + HSERO_RS14210 0.62 -3.3 3,230,940 + HSERO_RS14210 0.65 -3.2 3,230,940 + HSERO_RS14210 0.65 -1.3 3,231,097 + HSERO_RS14210 0.79 -4.2 3,231,099 + HSERO_RS14210 0.79 -3.5 3,231,519 + HSERO_RS14215 0.36 -3.3 3,231,569 + HSERO_RS14215 0.51 -3.9 3,231,651 - HSERO_RS14215 0.76 -2.0 3,231,724 + -0.8 3,231,724 + +0.2 3,231,932 - HSERO_RS14220 0.21 -3.2 3,231,947 + HSERO_RS14220 0.23 -4.4 3,231,960 - HSERO_RS14220 0.24 -3.7 3,232,045 - HSERO_RS14220 0.35 -5.1 3,232,055 + HSERO_RS14220 0.36 -3.0 3,232,137 + HSERO_RS14220 0.45 +1.6 3,232,383 - HSERO_RS14220 0.74 -3.6 3,232,409 + HSERO_RS14220 0.78 -3.7 3,232,473 - HSERO_RS14220 0.85 -4.5 3,232,473 - HSERO_RS14220 0.85 -5.2 3,232,552 + -2.6 3,232,611 - -3.2 3,232,695 + -3.9 3,232,760 + HSERO_RS14225 0.12 -4.3 3,232,768 - HSERO_RS14225 0.13 -3.1 3,232,825 + HSERO_RS14225 0.18 -3.8 3,232,910 + HSERO_RS14225 0.26 -4.0 3,232,918 - HSERO_RS14225 0.26 -4.7 3,233,051 + HSERO_RS14225 0.38 -5.6 3,233,548 + HSERO_RS14225 0.84 -2.7
Or see this region's nucleotide sequence