Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14200 and HSERO_RS14205 are separated by 51 nucleotides HSERO_RS14205 and HSERO_RS14210 are separated by 209 nucleotides HSERO_RS14210 and HSERO_RS14215 are separated by 67 nucleotides HSERO_RS14215 and HSERO_RS14220 are separated by 22 nucleotides
HSERO_RS14200: HSERO_RS14200 - molecular chaperone HscA, at 3,227,675 to 3,229,603
_RS14200
HSERO_RS14205: HSERO_RS14205 - ferredoxin [2Fe-2S]-type protein, at 3,229,655 to 3,229,993
_RS14205
HSERO_RS14210: HSERO_RS14210 - homocitrate synthase, at 3,230,203 to 3,231,333
_RS14210
HSERO_RS14215: HSERO_RS14215 - nitrogen fixation protein NifW, at 3,231,401 to 3,231,730
_RS14215
HSERO_RS14220: HSERO_RS14220 - protein fixA, at 3,231,753 to 3,232,598
_RS14220
Position (kb) 3230
3231
3232 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 3229.229 kb on - strand, within HSERO_RS14200 at 3229.274 kb on - strand, within HSERO_RS14200 at 3229.417 kb on + strand at 3229.425 kb on - strand at 3229.427 kb on - strand at 3229.561 kb on + strand at 3229.569 kb on - strand at 3229.680 kb on + strand at 3229.724 kb on + strand at 3229.777 kb on - strand, within HSERO_RS14205 at 3229.931 kb on + strand, within HSERO_RS14205 at 3229.957 kb on + strand, within HSERO_RS14205 at 3229.965 kb on - strand at 3229.965 kb on - strand at 3230.059 kb on + strand at 3230.067 kb on - strand at 3230.067 kb on - strand at 3230.219 kb on + strand at 3230.227 kb on - strand at 3230.227 kb on - strand at 3230.227 kb on - strand at 3230.237 kb on + strand at 3230.254 kb on - strand at 3230.370 kb on + strand, within HSERO_RS14210 at 3230.651 kb on - strand, within HSERO_RS14210 at 3230.804 kb on - strand, within HSERO_RS14210 at 3230.836 kb on + strand, within HSERO_RS14210 at 3230.841 kb on + strand, within HSERO_RS14210 at 3230.849 kb on - strand, within HSERO_RS14210 at 3230.908 kb on + strand, within HSERO_RS14210 at 3230.940 kb on + strand, within HSERO_RS14210 at 3230.940 kb on + strand, within HSERO_RS14210 at 3231.097 kb on + strand, within HSERO_RS14210 at 3231.099 kb on + strand, within HSERO_RS14210 at 3231.519 kb on + strand, within HSERO_RS14215 at 3231.569 kb on + strand, within HSERO_RS14215 at 3231.651 kb on - strand, within HSERO_RS14215 at 3231.724 kb on + strand at 3231.724 kb on + strand at 3231.932 kb on - strand, within HSERO_RS14220 at 3231.947 kb on + strand, within HSERO_RS14220 at 3231.960 kb on - strand, within HSERO_RS14220 at 3232.045 kb on - strand, within HSERO_RS14220 at 3232.055 kb on + strand, within HSERO_RS14220 at 3232.137 kb on + strand, within HSERO_RS14220
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,229,229 - HSERO_RS14200 0.81 -1.1 3,229,274 - HSERO_RS14200 0.83 -0.8 3,229,417 + -0.1 3,229,425 - -1.2 3,229,427 - -0.7 3,229,561 + -0.1 3,229,569 - -0.4 3,229,680 + -1.2 3,229,724 + -0.0 3,229,777 - HSERO_RS14205 0.36 -0.9 3,229,931 + HSERO_RS14205 0.81 -1.6 3,229,957 + HSERO_RS14205 0.89 -0.8 3,229,965 - -1.2 3,229,965 - -0.2 3,230,059 + -2.6 3,230,067 - -4.5 3,230,067 - -4.1 3,230,219 + -3.5 3,230,227 - -2.0 3,230,227 - -4.1 3,230,227 - -2.5 3,230,237 + -4.1 3,230,254 - -4.5 3,230,370 + HSERO_RS14210 0.15 -3.4 3,230,651 - HSERO_RS14210 0.40 -3.1 3,230,804 - HSERO_RS14210 0.53 -4.2 3,230,836 + HSERO_RS14210 0.56 -5.1 3,230,841 + HSERO_RS14210 0.56 -2.2 3,230,849 - HSERO_RS14210 0.57 -2.9 3,230,908 + HSERO_RS14210 0.62 -3.3 3,230,940 + HSERO_RS14210 0.65 -3.2 3,230,940 + HSERO_RS14210 0.65 -1.3 3,231,097 + HSERO_RS14210 0.79 -4.2 3,231,099 + HSERO_RS14210 0.79 -3.5 3,231,519 + HSERO_RS14215 0.36 -3.3 3,231,569 + HSERO_RS14215 0.51 -3.9 3,231,651 - HSERO_RS14215 0.76 -2.0 3,231,724 + -0.8 3,231,724 + +0.2 3,231,932 - HSERO_RS14220 0.21 -3.2 3,231,947 + HSERO_RS14220 0.23 -4.4 3,231,960 - HSERO_RS14220 0.24 -3.7 3,232,045 - HSERO_RS14220 0.35 -5.1 3,232,055 + HSERO_RS14220 0.36 -3.0 3,232,137 + HSERO_RS14220 0.45 +1.6
Or see this region's nucleotide sequence