Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS14180

Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS14175 and HSERO_RS14180 are separated by 516 nucleotidesHSERO_RS14180 and HSERO_RS14185 are separated by 13 nucleotidesHSERO_RS14185 and HSERO_RS14190 are separated by 43 nucleotidesHSERO_RS14190 and HSERO_RS14195 are separated by 12 nucleotides HSERO_RS14175: HSERO_RS14175 - lactoylglutathione lyase, at 3,224,427 to 3,224,738 _RS14175 HSERO_RS14180: HSERO_RS14180 - cysteine desulfurase, at 3,225,255 to 3,226,475 _RS14180 HSERO_RS14185: HSERO_RS14185 - FeS cluster assembly scaffold IscU, at 3,226,489 to 3,226,938 _RS14185 HSERO_RS14190: HSERO_RS14190 - iron--sulfur cluster insertion protein ErpA, at 3,226,982 to 3,227,338 _RS14190 HSERO_RS14195: HSERO_RS14195 - heme biosynthesis protein HemY, at 3,227,351 to 3,227,671 _RS14195 Position (kb) 3225 3226 3227Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3224.288 kb on + strandat 3224.339 kb on + strandat 3224.388 kb on - strandat 3224.568 kb on - strand, within HSERO_RS14175at 3224.905 kb on - strandat 3225.228 kb on + strandat 3225.228 kb on + strandat 3225.236 kb on + strandat 3225.313 kb on + strandat 3225.517 kb on + strand, within HSERO_RS14180at 3225.548 kb on + strand, within HSERO_RS14180at 3225.598 kb on + strand, within HSERO_RS14180at 3225.613 kb on + strand, within HSERO_RS14180at 3225.613 kb on + strand, within HSERO_RS14180at 3225.705 kb on + strand, within HSERO_RS14180at 3225.746 kb on - strand, within HSERO_RS14180at 3225.746 kb on - strand, within HSERO_RS14180at 3225.781 kb on + strand, within HSERO_RS14180at 3225.850 kb on - strand, within HSERO_RS14180at 3225.893 kb on + strand, within HSERO_RS14180at 3225.950 kb on - strand, within HSERO_RS14180at 3226.199 kb on + strand, within HSERO_RS14180at 3226.228 kb on + strand, within HSERO_RS14180at 3226.236 kb on - strand, within HSERO_RS14180at 3226.239 kb on + strand, within HSERO_RS14180at 3226.255 kb on + strand, within HSERO_RS14180at 3226.263 kb on - strand, within HSERO_RS14180at 3226.263 kb on - strand, within HSERO_RS14180at 3226.267 kb on + strand, within HSERO_RS14180at 3226.275 kb on - strand, within HSERO_RS14180at 3226.275 kb on - strand, within HSERO_RS14180at 3226.355 kb on - strandat 3226.392 kb on + strandat 3226.485 kb on + strandat 3226.813 kb on - strand, within HSERO_RS14185at 3227.131 kb on - strand, within HSERO_RS14190

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
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3,224,288 + +0.8
3,224,339 + -0.7
3,224,388 - +0.3
3,224,568 - HSERO_RS14175 0.45 +0.2
3,224,905 - +2.2
3,225,228 + -1.9
3,225,228 + -1.8
3,225,236 + -1.6
3,225,313 + -2.6
3,225,517 + HSERO_RS14180 0.21 -1.2
3,225,548 + HSERO_RS14180 0.24 -0.5
3,225,598 + HSERO_RS14180 0.28 -1.2
3,225,613 + HSERO_RS14180 0.29 -1.5
3,225,613 + HSERO_RS14180 0.29 -1.1
3,225,705 + HSERO_RS14180 0.37 -0.3
3,225,746 - HSERO_RS14180 0.40 -4.5
3,225,746 - HSERO_RS14180 0.40 -2.4
3,225,781 + HSERO_RS14180 0.43 -0.8
3,225,850 - HSERO_RS14180 0.49 -3.2
3,225,893 + HSERO_RS14180 0.52 -2.0
3,225,950 - HSERO_RS14180 0.57 -2.5
3,226,199 + HSERO_RS14180 0.77 -1.2
3,226,228 + HSERO_RS14180 0.80 +0.7
3,226,236 - HSERO_RS14180 0.80 -2.3
3,226,239 + HSERO_RS14180 0.81 -0.2
3,226,255 + HSERO_RS14180 0.82 -0.7
3,226,263 - HSERO_RS14180 0.83 -1.9
3,226,263 - HSERO_RS14180 0.83 -2.1
3,226,267 + HSERO_RS14180 0.83 -0.1
3,226,275 - HSERO_RS14180 0.84 -1.1
3,226,275 - HSERO_RS14180 0.84 -2.0
3,226,355 - -1.9
3,226,392 + +0.7
3,226,485 + -1.6
3,226,813 - HSERO_RS14185 0.72 -2.7
3,227,131 - HSERO_RS14190 0.42 -2.8

Or see this region's nucleotide sequence