Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14175 and HSERO_RS14180 are separated by 516 nucleotides HSERO_RS14180 and HSERO_RS14185 are separated by 13 nucleotides HSERO_RS14185 and HSERO_RS14190 are separated by 43 nucleotides HSERO_RS14190 and HSERO_RS14195 are separated by 12 nucleotides
HSERO_RS14175: HSERO_RS14175 - lactoylglutathione lyase, at 3,224,427 to 3,224,738
_RS14175
HSERO_RS14180: HSERO_RS14180 - cysteine desulfurase, at 3,225,255 to 3,226,475
_RS14180
HSERO_RS14185: HSERO_RS14185 - FeS cluster assembly scaffold IscU, at 3,226,489 to 3,226,938
_RS14185
HSERO_RS14190: HSERO_RS14190 - iron--sulfur cluster insertion protein ErpA, at 3,226,982 to 3,227,338
_RS14190
HSERO_RS14195: HSERO_RS14195 - heme biosynthesis protein HemY, at 3,227,351 to 3,227,671
_RS14195
Position (kb)
3225
3226
3227 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3224.288 kb on + strand at 3224.339 kb on + strand at 3224.388 kb on - strand at 3224.568 kb on - strand, within HSERO_RS14175 at 3224.905 kb on - strand at 3225.228 kb on + strand at 3225.228 kb on + strand at 3225.236 kb on + strand at 3225.313 kb on + strand at 3225.517 kb on + strand, within HSERO_RS14180 at 3225.548 kb on + strand, within HSERO_RS14180 at 3225.598 kb on + strand, within HSERO_RS14180 at 3225.613 kb on + strand, within HSERO_RS14180 at 3225.613 kb on + strand, within HSERO_RS14180 at 3225.705 kb on + strand, within HSERO_RS14180 at 3225.746 kb on - strand, within HSERO_RS14180 at 3225.746 kb on - strand, within HSERO_RS14180 at 3225.781 kb on + strand, within HSERO_RS14180 at 3225.850 kb on - strand, within HSERO_RS14180 at 3225.893 kb on + strand, within HSERO_RS14180 at 3225.950 kb on - strand, within HSERO_RS14180 at 3226.199 kb on + strand, within HSERO_RS14180 at 3226.228 kb on + strand, within HSERO_RS14180 at 3226.236 kb on - strand, within HSERO_RS14180 at 3226.239 kb on + strand, within HSERO_RS14180 at 3226.255 kb on + strand, within HSERO_RS14180 at 3226.263 kb on - strand, within HSERO_RS14180 at 3226.263 kb on - strand, within HSERO_RS14180 at 3226.267 kb on + strand, within HSERO_RS14180 at 3226.275 kb on - strand, within HSERO_RS14180 at 3226.275 kb on - strand, within HSERO_RS14180 at 3226.355 kb on - strand at 3226.392 kb on + strand at 3226.485 kb on + strand at 3226.813 kb on - strand, within HSERO_RS14185 at 3227.131 kb on - strand, within HSERO_RS14190
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,224,288 + +0.8 3,224,339 + -0.7 3,224,388 - +0.3 3,224,568 - HSERO_RS14175 0.45 +0.2 3,224,905 - +2.2 3,225,228 + -1.9 3,225,228 + -1.8 3,225,236 + -1.6 3,225,313 + -2.6 3,225,517 + HSERO_RS14180 0.21 -1.2 3,225,548 + HSERO_RS14180 0.24 -0.5 3,225,598 + HSERO_RS14180 0.28 -1.2 3,225,613 + HSERO_RS14180 0.29 -1.5 3,225,613 + HSERO_RS14180 0.29 -1.1 3,225,705 + HSERO_RS14180 0.37 -0.3 3,225,746 - HSERO_RS14180 0.40 -4.5 3,225,746 - HSERO_RS14180 0.40 -2.4 3,225,781 + HSERO_RS14180 0.43 -0.8 3,225,850 - HSERO_RS14180 0.49 -3.2 3,225,893 + HSERO_RS14180 0.52 -2.0 3,225,950 - HSERO_RS14180 0.57 -2.5 3,226,199 + HSERO_RS14180 0.77 -1.2 3,226,228 + HSERO_RS14180 0.80 +0.7 3,226,236 - HSERO_RS14180 0.80 -2.3 3,226,239 + HSERO_RS14180 0.81 -0.2 3,226,255 + HSERO_RS14180 0.82 -0.7 3,226,263 - HSERO_RS14180 0.83 -1.9 3,226,263 - HSERO_RS14180 0.83 -2.1 3,226,267 + HSERO_RS14180 0.83 -0.1 3,226,275 - HSERO_RS14180 0.84 -1.1 3,226,275 - HSERO_RS14180 0.84 -2.0 3,226,355 - -1.9 3,226,392 + +0.7 3,226,485 + -1.6 3,226,813 - HSERO_RS14185 0.72 -2.7 3,227,131 - HSERO_RS14190 0.42 -2.8
Or see this region's nucleotide sequence