Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14005 and HSERO_RS14010 are separated by 105 nucleotides HSERO_RS14010 and HSERO_RS14015 are separated by 131 nucleotides HSERO_RS14015 and HSERO_RS14020 are separated by 11 nucleotides
HSERO_RS14005: HSERO_RS14005 - carboxylesterase, at 3,187,457 to 3,188,368
_RS14005
HSERO_RS14010: HSERO_RS14010 - histidine kinase, at 3,188,474 to 3,188,992
_RS14010
HSERO_RS14015: HSERO_RS14015 - hisitidine kinase, at 3,189,124 to 3,189,558
_RS14015
HSERO_RS14020: HSERO_RS14020 - LysR family transcriptional regulator, at 3,189,570 to 3,190,487
_RS14020
Position (kb)
3189
3190 Strain fitness (log2 ratio)
-1
0
1 at 3188.202 kb on - strand, within HSERO_RS14005 at 3188.300 kb on - strand at 3188.345 kb on + strand at 3188.608 kb on - strand, within HSERO_RS14010 at 3188.625 kb on - strand, within HSERO_RS14010 at 3188.645 kb on + strand, within HSERO_RS14010 at 3188.694 kb on - strand, within HSERO_RS14010 at 3188.695 kb on + strand, within HSERO_RS14010 at 3188.766 kb on - strand, within HSERO_RS14010 at 3188.814 kb on - strand, within HSERO_RS14010 at 3188.922 kb on + strand, within HSERO_RS14010 at 3189.508 kb on - strand, within HSERO_RS14015 at 3189.519 kb on - strand at 3189.533 kb on + strand at 3189.552 kb on + strand at 3189.560 kb on - strand at 3189.580 kb on + strand at 3189.728 kb on - strand, within HSERO_RS14020 at 3189.741 kb on - strand, within HSERO_RS14020 at 3189.843 kb on - strand, within HSERO_RS14020 at 3189.958 kb on - strand, within HSERO_RS14020 at 3190.215 kb on + strand, within HSERO_RS14020 at 3190.242 kb on - strand, within HSERO_RS14020 at 3190.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,188,202 - HSERO_RS14005 0.82 +0.0 3,188,300 - +0.0 3,188,345 + +0.3 3,188,608 - HSERO_RS14010 0.26 +0.6 3,188,625 - HSERO_RS14010 0.29 -0.3 3,188,645 + HSERO_RS14010 0.33 +0.2 3,188,694 - HSERO_RS14010 0.42 -0.3 3,188,695 + HSERO_RS14010 0.43 +0.2 3,188,766 - HSERO_RS14010 0.56 -0.2 3,188,814 - HSERO_RS14010 0.66 -0.1 3,188,922 + HSERO_RS14010 0.86 -0.3 3,189,508 - HSERO_RS14015 0.88 +0.5 3,189,519 - -0.7 3,189,533 + +0.1 3,189,552 + -0.4 3,189,560 - +0.4 3,189,580 + +0.4 3,189,728 - HSERO_RS14020 0.17 +0.1 3,189,741 - HSERO_RS14020 0.19 +0.8 3,189,843 - HSERO_RS14020 0.30 -0.6 3,189,958 - HSERO_RS14020 0.42 -0.3 3,190,215 + HSERO_RS14020 0.70 -0.5 3,190,242 - HSERO_RS14020 0.73 +0.2 3,190,404 - -0.2
Or see this region's nucleotide sequence