Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14000 and HSERO_RS14005 are separated by 385 nucleotides HSERO_RS14005 and HSERO_RS14010 are separated by 105 nucleotides HSERO_RS14010 and HSERO_RS14015 are separated by 131 nucleotides
HSERO_RS14000: HSERO_RS14000 - hypothetical protein, at 3,186,460 to 3,187,071
_RS14000
HSERO_RS14005: HSERO_RS14005 - carboxylesterase, at 3,187,457 to 3,188,368
_RS14005
HSERO_RS14010: HSERO_RS14010 - histidine kinase, at 3,188,474 to 3,188,992
_RS14010
HSERO_RS14015: HSERO_RS14015 - hisitidine kinase, at 3,189,124 to 3,189,558
_RS14015
Position (kb)
3187
3188
3189 Strain fitness (log2 ratio)
-2
-1
0
1 at 3186.569 kb on + strand, within HSERO_RS14000 at 3186.787 kb on + strand, within HSERO_RS14000 at 3186.787 kb on + strand, within HSERO_RS14000 at 3186.787 kb on + strand, within HSERO_RS14000 at 3187.297 kb on + strand at 3187.297 kb on + strand at 3187.614 kb on - strand, within HSERO_RS14005 at 3187.702 kb on - strand, within HSERO_RS14005 at 3187.709 kb on + strand, within HSERO_RS14005 at 3187.720 kb on - strand, within HSERO_RS14005 at 3187.845 kb on - strand, within HSERO_RS14005 at 3187.848 kb on - strand, within HSERO_RS14005 at 3188.098 kb on + strand, within HSERO_RS14005 at 3188.202 kb on - strand, within HSERO_RS14005 at 3188.300 kb on - strand at 3188.345 kb on + strand at 3188.608 kb on - strand, within HSERO_RS14010 at 3188.625 kb on - strand, within HSERO_RS14010 at 3188.645 kb on + strand, within HSERO_RS14010 at 3188.694 kb on - strand, within HSERO_RS14010 at 3188.695 kb on + strand, within HSERO_RS14010 at 3188.766 kb on - strand, within HSERO_RS14010 at 3188.814 kb on - strand, within HSERO_RS14010 at 3188.922 kb on + strand, within HSERO_RS14010
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,186,569 + HSERO_RS14000 0.18 +0.4 3,186,787 + HSERO_RS14000 0.53 -2.6 3,186,787 + HSERO_RS14000 0.53 +0.4 3,186,787 + HSERO_RS14000 0.53 -0.0 3,187,297 + +0.2 3,187,297 + +0.4 3,187,614 - HSERO_RS14005 0.17 +0.6 3,187,702 - HSERO_RS14005 0.27 +0.6 3,187,709 + HSERO_RS14005 0.28 +0.8 3,187,720 - HSERO_RS14005 0.29 +0.3 3,187,845 - HSERO_RS14005 0.43 +0.1 3,187,848 - HSERO_RS14005 0.43 +0.1 3,188,098 + HSERO_RS14005 0.70 +1.2 3,188,202 - HSERO_RS14005 0.82 +0.0 3,188,300 - +0.0 3,188,345 + +0.3 3,188,608 - HSERO_RS14010 0.26 +0.6 3,188,625 - HSERO_RS14010 0.29 -0.3 3,188,645 + HSERO_RS14010 0.33 +0.2 3,188,694 - HSERO_RS14010 0.42 -0.3 3,188,695 + HSERO_RS14010 0.43 +0.2 3,188,766 - HSERO_RS14010 0.56 -0.2 3,188,814 - HSERO_RS14010 0.66 -0.1 3,188,922 + HSERO_RS14010 0.86 -0.3
Or see this region's nucleotide sequence