Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS13055 and HSERO_RS13060 are separated by 70 nucleotides HSERO_RS13060 and HSERO_RS13065 overlap by 4 nucleotides HSERO_RS13065 and HSERO_RS13070 are separated by 75 nucleotides
HSERO_RS13055: HSERO_RS13055 - energy transducer TonB, at 2,976,315 to 2,976,968
_RS13055
HSERO_RS13060: HSERO_RS13060 - biopolymer transporter ExbD, at 2,977,039 to 2,977,437
_RS13060
HSERO_RS13065: HSERO_RS13065 - biopolymer transporter, at 2,977,434 to 2,978,159
_RS13065
HSERO_RS13070: HSERO_RS13070 - TonB-denpendent receptor, at 2,978,235 to 2,981,030
_RS13070
Position (kb)
2977
2978
2979 Strain fitness (log2 ratio)
-2
-1
0
1 at 2976.456 kb on - strand, within HSERO_RS13055 at 2976.554 kb on + strand, within HSERO_RS13055 at 2976.912 kb on - strand at 2977.280 kb on - strand at 2977.483 kb on + strand at 2977.483 kb on + strand at 2977.736 kb on - strand, within HSERO_RS13065 at 2977.872 kb on + strand, within HSERO_RS13065 at 2977.872 kb on + strand, within HSERO_RS13065 at 2977.893 kb on - strand, within HSERO_RS13065 at 2977.930 kb on - strand, within HSERO_RS13065 at 2978.187 kb on - strand at 2978.211 kb on + strand at 2978.226 kb on + strand at 2978.269 kb on - strand at 2978.380 kb on + strand at 2978.390 kb on + strand at 2978.482 kb on - strand at 2978.571 kb on + strand, within HSERO_RS13070 at 2978.700 kb on + strand at 2978.705 kb on + strand, within HSERO_RS13070 at 2978.751 kb on + strand, within HSERO_RS13070 at 2978.806 kb on + strand, within HSERO_RS13070 at 2979.013 kb on - strand, within HSERO_RS13070 at 2979.123 kb on - strand, within HSERO_RS13070
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 2,976,456 - HSERO_RS13055 0.22 -0.3 2,976,554 + HSERO_RS13055 0.37 -0.6 2,976,912 - -0.3 2,977,280 - +0.5 2,977,483 + -0.8 2,977,483 + -1.8 2,977,736 - HSERO_RS13065 0.42 +0.9 2,977,872 + HSERO_RS13065 0.60 +0.0 2,977,872 + HSERO_RS13065 0.60 +0.5 2,977,893 - HSERO_RS13065 0.63 +1.1 2,977,930 - HSERO_RS13065 0.68 +0.0 2,978,187 - -0.0 2,978,211 + +0.4 2,978,226 + +0.1 2,978,269 - -0.2 2,978,380 + -0.2 2,978,390 + -0.3 2,978,482 - -0.1 2,978,571 + HSERO_RS13070 0.12 -0.5 2,978,700 + +0.5 2,978,705 + HSERO_RS13070 0.17 -0.1 2,978,751 + HSERO_RS13070 0.18 -0.8 2,978,806 + HSERO_RS13070 0.20 -0.4 2,979,013 - HSERO_RS13070 0.28 -0.5 2,979,123 - HSERO_RS13070 0.32 +0.5
Or see this region's nucleotide sequence