Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12085 and HSERO_RS12090 are separated by 134 nucleotides HSERO_RS12090 and HSERO_RS12095 are separated by 119 nucleotides HSERO_RS12095 and HSERO_RS12100 overlap by 4 nucleotides
HSERO_RS12085: HSERO_RS12085 - RNA polymerase sigma-E factor protein, at 2,745,958 to 2,746,518
_RS12085
HSERO_RS12090: HSERO_RS12090 - hypothetical protein, at 2,746,653 to 2,747,237
_RS12090
HSERO_RS12095: HSERO_RS12095 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18) (from data) , at 2,747,357 to 2,748,349
_RS12095
HSERO_RS12100: HSERO_RS12100 - oxidoreductase, at 2,748,346 to 2,749,371
_RS12100
Position (kb)
2747
2748
2749 Strain fitness (log2 ratio)
-1
0
1 at 2746.373 kb on + strand, within HSERO_RS12085 at 2746.376 kb on - strand, within HSERO_RS12085 at 2746.381 kb on - strand, within HSERO_RS12085 at 2746.473 kb on + strand at 2746.601 kb on + strand at 2746.652 kb on + strand at 2746.671 kb on - strand at 2746.689 kb on + strand at 2746.845 kb on + strand at 2746.846 kb on + strand, within HSERO_RS12090 at 2746.972 kb on - strand, within HSERO_RS12090 at 2747.148 kb on + strand, within HSERO_RS12090 at 2747.177 kb on - strand, within HSERO_RS12090 at 2747.240 kb on + strand at 2747.488 kb on + strand, within HSERO_RS12095 at 2747.520 kb on - strand, within HSERO_RS12095 at 2747.536 kb on - strand, within HSERO_RS12095 at 2747.566 kb on - strand, within HSERO_RS12095 at 2748.047 kb on + strand, within HSERO_RS12095 at 2748.445 kb on - strand at 2748.583 kb on + strand, within HSERO_RS12100 at 2748.659 kb on + strand, within HSERO_RS12100 at 2748.708 kb on + strand at 2748.890 kb on + strand, within HSERO_RS12100 at 2748.903 kb on - strand, within HSERO_RS12100 at 2749.089 kb on + strand, within HSERO_RS12100 at 2749.172 kb on + strand at 2749.172 kb on + strand, within HSERO_RS12100
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 2,746,373 + HSERO_RS12085 0.74 -0.1 2,746,376 - HSERO_RS12085 0.75 -0.9 2,746,381 - HSERO_RS12085 0.75 -0.0 2,746,473 + +0.0 2,746,601 + +0.3 2,746,652 + +0.6 2,746,671 - +1.7 2,746,689 + -0.9 2,746,845 + +0.2 2,746,846 + HSERO_RS12090 0.33 +0.6 2,746,972 - HSERO_RS12090 0.55 +0.0 2,747,148 + HSERO_RS12090 0.85 +0.5 2,747,177 - HSERO_RS12090 0.90 -0.1 2,747,240 + +0.2 2,747,488 + HSERO_RS12095 0.13 +0.0 2,747,520 - HSERO_RS12095 0.16 +1.0 2,747,536 - HSERO_RS12095 0.18 +1.1 2,747,566 - HSERO_RS12095 0.21 -0.2 2,748,047 + HSERO_RS12095 0.69 +0.1 2,748,445 - +0.2 2,748,583 + HSERO_RS12100 0.23 -0.1 2,748,659 + HSERO_RS12100 0.31 +0.4 2,748,708 + -0.6 2,748,890 + HSERO_RS12100 0.53 -0.4 2,748,903 - HSERO_RS12100 0.54 -0.2 2,749,089 + HSERO_RS12100 0.72 +0.0 2,749,172 + +0.4 2,749,172 + HSERO_RS12100 0.81 -0.4
Or see this region's nucleotide sequence