Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS11160 and HSERO_RS11165 are separated by 95 nucleotides HSERO_RS11165 and HSERO_RS11170 are separated by 55 nucleotides HSERO_RS11170 and HSERO_RS11175 are separated by 16 nucleotides HSERO_RS11175 and HSERO_RS11180 overlap by 47 nucleotides
HSERO_RS11160: HSERO_RS11160 - glutamine amidotransferase, at 2,519,779 to 2,520,498
_RS11160
HSERO_RS11165: HSERO_RS11165 - arylesterase, at 2,520,594 to 2,521,214
_RS11165
HSERO_RS11170: HSERO_RS11170 - ABC transporter ATP-binding protein, at 2,521,270 to 2,521,974
_RS11170
HSERO_RS11175: HSERO_RS11175 - multidrug DMT transporter permease, at 2,521,991 to 2,522,935
_RS11175
HSERO_RS11180: HSERO_RS11180 - sugar kinase, at 2,522,889 to 2,524,457
_RS11180
Position (kb)
2521
2522 Strain fitness (log2 ratio)
-1
0
1 at 2520.323 kb on - strand, within HSERO_RS11160 at 2520.644 kb on + strand at 2520.649 kb on + strand at 2520.835 kb on + strand, within HSERO_RS11165 at 2520.885 kb on - strand, within HSERO_RS11165 at 2520.966 kb on + strand, within HSERO_RS11165 at 2521.086 kb on - strand, within HSERO_RS11165 at 2521.285 kb on + strand at 2521.823 kb on + strand, within HSERO_RS11170 at 2521.886 kb on + strand, within HSERO_RS11170 at 2521.894 kb on - strand, within HSERO_RS11170 at 2522.304 kb on + strand, within HSERO_RS11175 at 2522.338 kb on - strand, within HSERO_RS11175 at 2522.351 kb on - strand, within HSERO_RS11175 at 2522.429 kb on - strand, within HSERO_RS11175 at 2522.595 kb on + strand, within HSERO_RS11175 at 2522.772 kb on - strand, within HSERO_RS11175 at 2522.788 kb on - strand, within HSERO_RS11175 at 2522.815 kb on + strand, within HSERO_RS11175 at 2522.861 kb on - strand at 2522.925 kb on + strand at 2522.930 kb on + strand at 2522.933 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 2,520,323 - HSERO_RS11160 0.76 -0.3 2,520,644 + -0.2 2,520,649 + +1.2 2,520,835 + HSERO_RS11165 0.39 +0.2 2,520,885 - HSERO_RS11165 0.47 +0.4 2,520,966 + HSERO_RS11165 0.60 +0.2 2,521,086 - HSERO_RS11165 0.79 +0.0 2,521,285 + +0.3 2,521,823 + HSERO_RS11170 0.78 +1.4 2,521,886 + HSERO_RS11170 0.87 -0.3 2,521,894 - HSERO_RS11170 0.89 -0.2 2,522,304 + HSERO_RS11175 0.33 +0.2 2,522,338 - HSERO_RS11175 0.37 +0.0 2,522,351 - HSERO_RS11175 0.38 +0.2 2,522,429 - HSERO_RS11175 0.46 -1.5 2,522,595 + HSERO_RS11175 0.64 +0.3 2,522,772 - HSERO_RS11175 0.83 -0.3 2,522,788 - HSERO_RS11175 0.84 +0.3 2,522,815 + HSERO_RS11175 0.87 +0.0 2,522,861 - +0.6 2,522,925 + +0.2 2,522,930 + -0.1 2,522,933 - -0.4
Or see this region's nucleotide sequence