Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS10270

Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS10260 and HSERO_RS10265 are separated by 46 nucleotidesHSERO_RS10265 and HSERO_RS10270 are separated by 19 nucleotidesHSERO_RS10270 and HSERO_RS10275 are separated by 2 nucleotides HSERO_RS10260: HSERO_RS10260 - flagellar biosynthesis protein FliP, at 2,314,327 to 2,315,061 _RS10260 HSERO_RS10265: HSERO_RS10265 - flagellar biosynthesis protein FliO, at 2,315,108 to 2,315,728 _RS10265 HSERO_RS10270: HSERO_RS10270 - flagellar motor switch protein FliN, at 2,315,748 to 2,316,203 _RS10270 HSERO_RS10275: HSERO_RS10275 - flagellar motor switch protein FliM, at 2,316,206 to 2,317,210 _RS10275 Position (kb) 2315 2316 2317Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2314.991 kb on + strandat 2315.098 kb on + strandat 2315.389 kb on + strand, within HSERO_RS10265at 2315.465 kb on - strand, within HSERO_RS10265at 2315.686 kb on + strandat 2315.686 kb on + strandat 2315.689 kb on - strandat 2315.694 kb on - strandat 2315.788 kb on - strandat 2316.118 kb on + strand, within HSERO_RS10270at 2316.862 kb on + strand, within HSERO_RS10275at 2316.870 kb on - strand, within HSERO_RS10275at 2316.893 kb on - strand, within HSERO_RS10275at 2316.989 kb on - strand, within HSERO_RS10275at 2317.109 kb on - strand, within HSERO_RS10275

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
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2,314,991 + -5.6
2,315,098 + -5.7
2,315,389 + HSERO_RS10265 0.45 -2.0
2,315,465 - HSERO_RS10265 0.57 -4.5
2,315,686 + -4.3
2,315,686 + -4.2
2,315,689 - -4.6
2,315,694 - -5.0
2,315,788 - -3.7
2,316,118 + HSERO_RS10270 0.81 -2.7
2,316,862 + HSERO_RS10275 0.65 -4.5
2,316,870 - HSERO_RS10275 0.66 -2.0
2,316,893 - HSERO_RS10275 0.68 -5.2
2,316,989 - HSERO_RS10275 0.78 -5.4
2,317,109 - HSERO_RS10275 0.90 -1.0

Or see this region's nucleotide sequence