Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10125 and HSERO_RS10130 are separated by 173 nucleotides HSERO_RS10130 and HSERO_RS10135 overlap by 8 nucleotides HSERO_RS10135 and HSERO_RS10140 are separated by 274 nucleotides
HSERO_RS10125: HSERO_RS10125 - chemotaxis protein, at 2,286,267 to 2,287,346
_RS10125
HSERO_RS10130: HSERO_RS10130 - chemotaxis protein CheY, at 2,287,520 to 2,287,915
_RS10130
HSERO_RS10135: HSERO_RS10135 - chemotaxis protein CheZ, at 2,287,908 to 2,288,567
_RS10135
HSERO_RS10140: HSERO_RS10140 - flagellar biosynthesis protein FlhB, at 2,288,842 to 2,290,017
_RS10140
Position (kb)
2287
2288
2289 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2287.054 kb on + strand, within HSERO_RS10125 at 2287.376 kb on - strand at 2287.548 kb on - strand at 2288.300 kb on + strand, within HSERO_RS10135 at 2288.485 kb on - strand, within HSERO_RS10135 at 2288.845 kb on + strand at 2288.875 kb on - strand at 2288.898 kb on - strand at 2288.993 kb on + strand, within HSERO_RS10140 at 2289.001 kb on - strand, within HSERO_RS10140 at 2289.016 kb on - strand, within HSERO_RS10140 at 2289.087 kb on - strand, within HSERO_RS10140 at 2289.098 kb on + strand, within HSERO_RS10140 at 2289.098 kb on + strand, within HSERO_RS10140 at 2289.098 kb on + strand, within HSERO_RS10140 at 2289.106 kb on - strand, within HSERO_RS10140 at 2289.106 kb on - strand at 2289.106 kb on - strand, within HSERO_RS10140 at 2289.106 kb on - strand, within HSERO_RS10140 at 2289.151 kb on - strand at 2289.205 kb on - strand, within HSERO_RS10140 at 2289.279 kb on + strand, within HSERO_RS10140 at 2289.291 kb on + strand, within HSERO_RS10140
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 2,287,054 + HSERO_RS10125 0.73 -2.9 2,287,376 - -0.4 2,287,548 - -2.1 2,288,300 + HSERO_RS10135 0.59 +0.5 2,288,485 - HSERO_RS10135 0.87 +0.6 2,288,845 + -1.1 2,288,875 - -3.7 2,288,898 - -4.0 2,288,993 + HSERO_RS10140 0.13 -4.6 2,289,001 - HSERO_RS10140 0.14 -5.2 2,289,016 - HSERO_RS10140 0.15 -4.0 2,289,087 - HSERO_RS10140 0.21 -5.0 2,289,098 + HSERO_RS10140 0.22 -6.6 2,289,098 + HSERO_RS10140 0.22 -3.2 2,289,098 + HSERO_RS10140 0.22 -3.8 2,289,106 - HSERO_RS10140 0.22 -5.5 2,289,106 - -1.4 2,289,106 - HSERO_RS10140 0.22 -5.3 2,289,106 - HSERO_RS10140 0.22 -3.8 2,289,151 - -1.0 2,289,205 - HSERO_RS10140 0.31 -3.7 2,289,279 + HSERO_RS10140 0.37 -4.6 2,289,291 + HSERO_RS10140 0.38 -3.7
Or see this region's nucleotide sequence