Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS07315 and HSERO_RS07320 are separated by 151 nucleotides HSERO_RS07320 and HSERO_RS07325 are separated by 121 nucleotides HSERO_RS07325 and HSERO_RS07330 are separated by 423 nucleotides
HSERO_RS07315: HSERO_RS07315 - dihydrolipoamide dehydrogenase, at 1,677,210 to 1,679,000
_RS07315
HSERO_RS07320: HSERO_RS07320 - hypothetical protein, at 1,679,152 to 1,679,625
_RS07320
HSERO_RS07325: HSERO_RS07325 - chemotaxis protein, at 1,679,747 to 1,681,306
_RS07325
HSERO_RS07330: HSERO_RS07330 - major facilitator transporter, at 1,681,730 to 1,683,028
_RS07330
Position (kb) 1679
1680
1681
1682 Strain fitness (log2 ratio) -1
0
1
2 at 1679.020 kb on - strand at 1679.067 kb on - strand at 1679.264 kb on + strand, within HSERO_RS07320 at 1679.657 kb on - strand at 1679.732 kb on + strand at 1679.839 kb on + strand at 1679.899 kb on + strand at 1679.992 kb on - strand, within HSERO_RS07325 at 1680.008 kb on + strand, within HSERO_RS07325 at 1680.093 kb on - strand, within HSERO_RS07325 at 1680.811 kb on - strand, within HSERO_RS07325 at 1680.841 kb on - strand, within HSERO_RS07325 at 1680.992 kb on - strand, within HSERO_RS07325 at 1681.056 kb on - strand, within HSERO_RS07325 at 1681.086 kb on + strand, within HSERO_RS07325 at 1681.086 kb on + strand, within HSERO_RS07325 at 1681.086 kb on + strand, within HSERO_RS07325 at 1681.342 kb on + strand at 1681.614 kb on + strand at 1681.687 kb on + strand at 1681.909 kb on + strand, within HSERO_RS07330 at 1681.918 kb on - strand, within HSERO_RS07330 at 1681.956 kb on + strand, within HSERO_RS07330 at 1681.985 kb on + strand, within HSERO_RS07330 at 1682.187 kb on - strand, within HSERO_RS07330
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 1,679,020 - +0.1 1,679,067 - +0.3 1,679,264 + HSERO_RS07320 0.24 -0.6 1,679,657 - +0.1 1,679,732 + -0.4 1,679,839 + +0.5 1,679,899 + -0.3 1,679,992 - HSERO_RS07325 0.16 +0.0 1,680,008 + HSERO_RS07325 0.17 -0.2 1,680,093 - HSERO_RS07325 0.22 -0.4 1,680,811 - HSERO_RS07325 0.68 -0.1 1,680,841 - HSERO_RS07325 0.70 -0.7 1,680,992 - HSERO_RS07325 0.80 +0.2 1,681,056 - HSERO_RS07325 0.84 -1.6 1,681,086 + HSERO_RS07325 0.86 -0.8 1,681,086 + HSERO_RS07325 0.86 -0.3 1,681,086 + HSERO_RS07325 0.86 -0.6 1,681,342 + +1.9 1,681,614 + +0.0 1,681,687 + -0.4 1,681,909 + HSERO_RS07330 0.14 -0.7 1,681,918 - HSERO_RS07330 0.14 -0.0 1,681,956 + HSERO_RS07330 0.17 -0.3 1,681,985 + HSERO_RS07330 0.20 +1.0 1,682,187 - HSERO_RS07330 0.35 -0.2
Or see this region's nucleotide sequence