Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05720 and HSERO_RS05725 are separated by 121 nucleotides HSERO_RS05725 and HSERO_RS05730 are separated by 381 nucleotides
HSERO_RS05720: HSERO_RS05720 - alanine racemase, at 1,299,562 to 1,300,692
_RS05720
HSERO_RS05725: HSERO_RS05725 - methyl-accepting chemotaxis protein, at 1,300,814 to 1,302,361
_RS05725
HSERO_RS05730: HSERO_RS05730 - glucarate dehydratase, at 1,302,743 to 1,304,017
_RS05730
Position (kb)
1300
1301
1302
1303 Strain fitness (log2 ratio)
-2
-1
0
1 at 1299.858 kb on - strand, within HSERO_RS05720 at 1299.924 kb on + strand, within HSERO_RS05720 at 1299.925 kb on + strand, within HSERO_RS05720 at 1300.028 kb on + strand, within HSERO_RS05720 at 1300.061 kb on - strand, within HSERO_RS05720 at 1300.078 kb on - strand, within HSERO_RS05720 at 1300.180 kb on - strand, within HSERO_RS05720 at 1300.509 kb on - strand, within HSERO_RS05720 at 1300.638 kb on + strand at 1300.659 kb on + strand at 1300.939 kb on - strand at 1300.975 kb on + strand, within HSERO_RS05725 at 1300.983 kb on - strand, within HSERO_RS05725 at 1300.983 kb on - strand, within HSERO_RS05725 at 1301.082 kb on + strand, within HSERO_RS05725 at 1301.203 kb on - strand at 1301.330 kb on - strand, within HSERO_RS05725 at 1301.386 kb on + strand, within HSERO_RS05725 at 1301.753 kb on + strand, within HSERO_RS05725 at 1301.833 kb on + strand, within HSERO_RS05725 at 1301.846 kb on + strand, within HSERO_RS05725 at 1301.855 kb on - strand, within HSERO_RS05725 at 1301.874 kb on - strand, within HSERO_RS05725 at 1302.034 kb on + strand, within HSERO_RS05725 at 1302.065 kb on + strand, within HSERO_RS05725 at 1302.112 kb on - strand, within HSERO_RS05725 at 1302.135 kb on + strand, within HSERO_RS05725 at 1302.207 kb on - strand at 1302.243 kb on + strand at 1302.322 kb on - strand at 1302.441 kb on + strand at 1302.494 kb on + strand at 1302.587 kb on + strand at 1302.626 kb on + strand at 1302.690 kb on - strand at 1302.796 kb on - strand at 1302.880 kb on + strand, within HSERO_RS05730 at 1302.888 kb on - strand, within HSERO_RS05730 at 1303.133 kb on - strand, within HSERO_RS05730 at 1303.163 kb on + strand, within HSERO_RS05730 at 1303.163 kb on + strand, within HSERO_RS05730 at 1303.163 kb on + strand, within HSERO_RS05730 at 1303.212 kb on - strand, within HSERO_RS05730
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 1,299,858 - HSERO_RS05720 0.26 +0.2 1,299,924 + HSERO_RS05720 0.32 -0.0 1,299,925 + HSERO_RS05720 0.32 +0.4 1,300,028 + HSERO_RS05720 0.41 -0.2 1,300,061 - HSERO_RS05720 0.44 -0.1 1,300,078 - HSERO_RS05720 0.46 -0.3 1,300,180 - HSERO_RS05720 0.55 -0.6 1,300,509 - HSERO_RS05720 0.84 -0.3 1,300,638 + -0.4 1,300,659 + +0.5 1,300,939 - +0.1 1,300,975 + HSERO_RS05725 0.10 +0.3 1,300,983 - HSERO_RS05725 0.11 +0.6 1,300,983 - HSERO_RS05725 0.11 -0.0 1,301,082 + HSERO_RS05725 0.17 -0.4 1,301,203 - +0.9 1,301,330 - HSERO_RS05725 0.33 -0.5 1,301,386 + HSERO_RS05725 0.37 +0.0 1,301,753 + HSERO_RS05725 0.61 -0.2 1,301,833 + HSERO_RS05725 0.66 +1.2 1,301,846 + HSERO_RS05725 0.67 -0.7 1,301,855 - HSERO_RS05725 0.67 +0.2 1,301,874 - HSERO_RS05725 0.68 +0.2 1,302,034 + HSERO_RS05725 0.79 +0.2 1,302,065 + HSERO_RS05725 0.81 +0.1 1,302,112 - HSERO_RS05725 0.84 +0.2 1,302,135 + HSERO_RS05725 0.85 -2.1 1,302,207 - -0.7 1,302,243 + +0.4 1,302,322 - -0.6 1,302,441 + +0.0 1,302,494 + -0.6 1,302,587 + +0.9 1,302,626 + +0.1 1,302,690 - +0.1 1,302,796 - +1.2 1,302,880 + HSERO_RS05730 0.11 -1.1 1,302,888 - HSERO_RS05730 0.11 +0.0 1,303,133 - HSERO_RS05730 0.31 -0.7 1,303,163 + HSERO_RS05730 0.33 -0.3 1,303,163 + HSERO_RS05730 0.33 +0.2 1,303,163 + HSERO_RS05730 0.33 -0.3 1,303,212 - HSERO_RS05730 0.37 -0.3
Or see this region's nucleotide sequence