Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04660 and HSERO_RS04665 are separated by 61 nucleotides HSERO_RS04665 and HSERO_RS04670 are separated by 70 nucleotides HSERO_RS04670 and HSERO_RS04675 are separated by 453 nucleotides
HSERO_RS04660: HSERO_RS04660 - nitrile hydratase, at 1,015,296 to 1,015,955
_RS04660
HSERO_RS04665: HSERO_RS04665 - nitrile hydratase, at 1,016,017 to 1,016,616
_RS04665
HSERO_RS04670: HSERO_RS04670 - amidase, at 1,016,687 to 1,018,201
_RS04670
HSERO_RS04675: HSERO_RS04675 - hypothetical protein, at 1,018,655 to 1,019,566
_RS04675
Position (kb)
1016
1017
1018
1019 Strain fitness (log2 ratio)
-1
0
1 at 1015.696 kb on - strand, within HSERO_RS04660 at 1015.801 kb on + strand, within HSERO_RS04660 at 1015.801 kb on + strand, within HSERO_RS04660 at 1015.847 kb on - strand, within HSERO_RS04660 at 1015.992 kb on + strand at 1015.997 kb on + strand at 1015.997 kb on + strand at 1016.002 kb on + strand at 1016.002 kb on + strand at 1016.024 kb on + strand at 1016.029 kb on + strand at 1016.057 kb on + strand at 1016.277 kb on - strand, within HSERO_RS04665 at 1016.438 kb on + strand, within HSERO_RS04665 at 1016.438 kb on + strand, within HSERO_RS04665 at 1016.580 kb on + strand at 1016.696 kb on + strand at 1016.730 kb on + strand at 1016.738 kb on - strand at 1016.797 kb on - strand at 1017.081 kb on - strand, within HSERO_RS04670 at 1017.413 kb on - strand, within HSERO_RS04670 at 1017.920 kb on - strand, within HSERO_RS04670 at 1017.949 kb on + strand, within HSERO_RS04670 at 1018.174 kb on + strand at 1018.386 kb on - strand at 1018.473 kb on - strand at 1018.542 kb on - strand at 1018.589 kb on + strand at 1018.589 kb on + strand at 1018.589 kb on + strand at 1018.589 kb on + strand at 1018.597 kb on - strand at 1018.597 kb on - strand at 1018.597 kb on - strand at 1018.609 kb on - strand at 1018.711 kb on - strand at 1018.872 kb on - strand, within HSERO_RS04675 at 1018.878 kb on + strand, within HSERO_RS04675 at 1018.969 kb on - strand, within HSERO_RS04675
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 1,015,696 - HSERO_RS04660 0.61 +0.1 1,015,801 + HSERO_RS04660 0.77 +0.2 1,015,801 + HSERO_RS04660 0.77 -0.3 1,015,847 - HSERO_RS04660 0.83 -0.3 1,015,992 + -0.0 1,015,997 + -0.0 1,015,997 + +0.1 1,016,002 + +0.0 1,016,002 + +0.3 1,016,024 + +0.6 1,016,029 + +0.3 1,016,057 + -0.1 1,016,277 - HSERO_RS04665 0.43 -0.4 1,016,438 + HSERO_RS04665 0.70 -0.2 1,016,438 + HSERO_RS04665 0.70 +0.1 1,016,580 + +1.1 1,016,696 + -0.3 1,016,730 + +0.1 1,016,738 - +0.2 1,016,797 - +0.1 1,017,081 - HSERO_RS04670 0.26 +0.4 1,017,413 - HSERO_RS04670 0.48 -0.5 1,017,920 - HSERO_RS04670 0.81 +0.0 1,017,949 + HSERO_RS04670 0.83 -0.5 1,018,174 + +0.2 1,018,386 - -0.4 1,018,473 - +0.2 1,018,542 - +0.2 1,018,589 + -0.6 1,018,589 + -0.0 1,018,589 + +0.1 1,018,589 + +0.3 1,018,597 - +0.3 1,018,597 - +0.1 1,018,597 - -0.1 1,018,609 - +0.3 1,018,711 - +0.2 1,018,872 - HSERO_RS04675 0.24 +0.2 1,018,878 + HSERO_RS04675 0.24 -0.3 1,018,969 - HSERO_RS04675 0.34 -0.7
Or see this region's nucleotide sequence