Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04210 and HSERO_RS04215 overlap by 37 nucleotides HSERO_RS04215 and HSERO_RS04220 are separated by 67 nucleotides HSERO_RS04220 and HSERO_RS04225 are separated by 12 nucleotides HSERO_RS04225 and HSERO_RS04230 are separated by 15 nucleotides
HSERO_RS04210: HSERO_RS04210 - N-acetylmuramoyl-L-alanine amidase, at 925,067 to 926,428
_RS04210
HSERO_RS04215: HSERO_RS04215 - hydrolase, at 926,392 to 926,877
_RS04215
HSERO_RS04220: HSERO_RS04220 - epoxyqueuosine reductase, at 926,945 to 928,090
_RS04220
HSERO_RS04225: HSERO_RS04225 - permease, at 928,103 to 928,993
_RS04225
HSERO_RS04230: HSERO_RS04230 - LuxR family transcriptional regulator, at 929,009 to 929,599
_RS04230
Position (kb) 926
927
928
929 Strain fitness (log2 ratio) -1
0
1 at 927.125 kb on + strand, within HSERO_RS04220 at 927.125 kb on + strand, within HSERO_RS04220 at 928.032 kb on + strand at 928.119 kb on + strand at 928.191 kb on - strand at 928.444 kb on + strand, within HSERO_RS04225 at 928.489 kb on + strand, within HSERO_RS04225 at 928.666 kb on - strand, within HSERO_RS04225 at 928.720 kb on - strand, within HSERO_RS04225 at 928.733 kb on - strand, within HSERO_RS04225 at 928.801 kb on - strand, within HSERO_RS04225 at 928.835 kb on - strand, within HSERO_RS04225 at 928.918 kb on + strand at 928.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 927,125 + HSERO_RS04220 0.16 +0.5 927,125 + HSERO_RS04220 0.16 +0.5 928,032 + +0.5 928,119 + -0.2 928,191 - +0.1 928,444 + HSERO_RS04225 0.38 -0.2 928,489 + HSERO_RS04225 0.43 -0.2 928,666 - HSERO_RS04225 0.63 -0.1 928,720 - HSERO_RS04225 0.69 -0.4 928,733 - HSERO_RS04225 0.71 +0.6 928,801 - HSERO_RS04225 0.78 +0.2 928,835 - HSERO_RS04225 0.82 -1.5 928,918 + -0.3 928,937 - +0.3
Or see this region's nucleotide sequence