Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02045 and HSERO_RS02050 are separated by 163 nucleotides HSERO_RS02050 and HSERO_RS02055 are separated by 178 nucleotides HSERO_RS02055 and HSERO_RS02060 are separated by 97 nucleotides
HSERO_RS02045: HSERO_RS02045 - toxin HipA, at 446,069 to 447,436
_RS02045
HSERO_RS02050: HSERO_RS02050 - tRNA-dihydrouridine synthase B, at 447,600 to 448,622
_RS02050
HSERO_RS02055: HSERO_RS02055 - Fis family transcriptional regulator, at 448,801 to 449,034
_RS02055
HSERO_RS02060: HSERO_RS02060 - purine biosynthesis protein purH, at 449,132 to 450,703
_RS02060
Position (kb) 447
448
449 Strain fitness (log2 ratio) -1
0
1
2 at 446.631 kb on + strand, within HSERO_RS02045 at 447.012 kb on - strand, within HSERO_RS02045 at 447.080 kb on - strand, within HSERO_RS02045 at 447.096 kb on - strand, within HSERO_RS02045 at 447.096 kb on - strand, within HSERO_RS02045 at 447.283 kb on - strand, within HSERO_RS02045 at 447.311 kb on + strand at 447.394 kb on - strand at 447.408 kb on + strand at 447.420 kb on - strand at 447.697 kb on + strand at 447.703 kb on + strand, within HSERO_RS02050 at 447.879 kb on + strand, within HSERO_RS02050 at 447.882 kb on + strand, within HSERO_RS02050 at 447.984 kb on - strand, within HSERO_RS02050 at 448.370 kb on + strand, within HSERO_RS02050 at 448.647 kb on + strand at 448.647 kb on + strand at 448.662 kb on + strand at 448.670 kb on - strand at 448.727 kb on - strand at 448.770 kb on - strand at 448.770 kb on - strand at 448.780 kb on - strand at 449.066 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 446,631 + HSERO_RS02045 0.41 +1.0 447,012 - HSERO_RS02045 0.69 +0.4 447,080 - HSERO_RS02045 0.74 -0.8 447,096 - HSERO_RS02045 0.75 -0.0 447,096 - HSERO_RS02045 0.75 -0.3 447,283 - HSERO_RS02045 0.89 -0.0 447,311 + +1.1 447,394 - +0.1 447,408 + -0.3 447,420 - -0.6 447,697 + +0.1 447,703 + HSERO_RS02050 0.10 +0.0 447,879 + HSERO_RS02050 0.27 +0.0 447,882 + HSERO_RS02050 0.28 -0.3 447,984 - HSERO_RS02050 0.38 +2.1 448,370 + HSERO_RS02050 0.75 +0.4 448,647 + -0.3 448,647 + +0.2 448,662 + +0.0 448,670 - +2.1 448,727 - +1.5 448,770 - +2.3 448,770 - +1.8 448,780 - +0.1 449,066 - -0.7
Or see this region's nucleotide sequence