Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01285 and HSERO_RS01290 are separated by 102 nucleotides HSERO_RS01290 and HSERO_RS01295 are separated by 237 nucleotides HSERO_RS01295 and HSERO_RS01300 are separated by 174 nucleotides
HSERO_RS01285: HSERO_RS01285 - diguanylate cyclase, at 272,282 to 273,436
_RS01285
HSERO_RS01290: HSERO_RS01290 - hypothetical protein, at 273,539 to 273,724
_RS01290
HSERO_RS01295: HSERO_RS01295 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 273,962 to 275,932
_RS01295
HSERO_RS01300: HSERO_RS01300 - phosphate acetyltransferase, at 276,107 to 277,066
_RS01300
Position (kb) 273
274
275
276 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 272.987 kb on + strand, within HSERO_RS01285 at 272.995 kb on - strand, within HSERO_RS01285 at 273.000 kb on + strand, within HSERO_RS01285 at 273.107 kb on - strand, within HSERO_RS01285 at 273.207 kb on - strand, within HSERO_RS01285 at 273.286 kb on + strand, within HSERO_RS01285 at 273.406 kb on + strand at 273.407 kb on + strand at 273.451 kb on + strand at 273.451 kb on + strand at 273.451 kb on + strand at 273.459 kb on - strand at 273.485 kb on - strand at 273.515 kb on - strand at 273.608 kb on - strand, within HSERO_RS01290 at 273.682 kb on + strand, within HSERO_RS01290 at 273.682 kb on - strand, within HSERO_RS01290 at 273.732 kb on - strand at 273.950 kb on + strand at 273.950 kb on - strand at 274.027 kb on + strand at 274.027 kb on + strand at 274.091 kb on - strand at 274.123 kb on + strand at 274.123 kb on + strand at 274.123 kb on + strand at 274.131 kb on - strand at 274.242 kb on + strand, within HSERO_RS01295 at 274.304 kb on + strand, within HSERO_RS01295 at 274.392 kb on + strand, within HSERO_RS01295 at 274.455 kb on + strand, within HSERO_RS01295 at 274.463 kb on - strand, within HSERO_RS01295 at 274.465 kb on + strand, within HSERO_RS01295 at 274.490 kb on + strand at 274.544 kb on - strand, within HSERO_RS01295 at 274.548 kb on + strand, within HSERO_RS01295 at 274.590 kb on - strand, within HSERO_RS01295 at 274.723 kb on + strand, within HSERO_RS01295 at 274.743 kb on + strand, within HSERO_RS01295 at 274.782 kb on - strand, within HSERO_RS01295 at 274.787 kb on - strand, within HSERO_RS01295 at 274.795 kb on - strand, within HSERO_RS01295 at 275.164 kb on + strand, within HSERO_RS01295 at 275.321 kb on - strand, within HSERO_RS01295 at 275.584 kb on + strand, within HSERO_RS01295 at 275.584 kb on + strand, within HSERO_RS01295 at 275.819 kb on + strand at 275.926 kb on + strand at 275.934 kb on - strand at 275.990 kb on - strand at 276.010 kb on - strand at 276.015 kb on + strand at 276.085 kb on - strand at 276.090 kb on + strand at 276.264 kb on - strand, within HSERO_RS01300 at 276.269 kb on - strand, within HSERO_RS01300 at 276.415 kb on + strand, within HSERO_RS01300 at 276.455 kb on + strand, within HSERO_RS01300 at 276.503 kb on + strand, within HSERO_RS01300 at 276.536 kb on - strand, within HSERO_RS01300 at 276.536 kb on - strand, within HSERO_RS01300 at 276.551 kb on - strand, within HSERO_RS01300 at 276.683 kb on - strand, within HSERO_RS01300
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 272,987 + HSERO_RS01285 0.61 +0.2 272,995 - HSERO_RS01285 0.62 +0.5 273,000 + HSERO_RS01285 0.62 -0.2 273,107 - HSERO_RS01285 0.71 +0.6 273,207 - HSERO_RS01285 0.80 -0.3 273,286 + HSERO_RS01285 0.87 -0.1 273,406 + -0.4 273,407 + +0.1 273,451 + -0.0 273,451 + -0.2 273,451 + +0.5 273,459 - +0.4 273,485 - -0.1 273,515 - -0.0 273,608 - HSERO_RS01290 0.37 -0.2 273,682 + HSERO_RS01290 0.77 -1.0 273,682 - HSERO_RS01290 0.77 -0.8 273,732 - +0.2 273,950 + +3.5 273,950 - +3.3 274,027 + +2.7 274,027 + +3.0 274,091 - +3.7 274,123 + +3.2 274,123 + +3.1 274,123 + +2.4 274,131 - +3.1 274,242 + HSERO_RS01295 0.14 +3.3 274,304 + HSERO_RS01295 0.17 +3.2 274,392 + HSERO_RS01295 0.22 +2.5 274,455 + HSERO_RS01295 0.25 +3.7 274,463 - HSERO_RS01295 0.25 +3.5 274,465 + HSERO_RS01295 0.26 +3.3 274,490 + +2.9 274,544 - HSERO_RS01295 0.30 +3.2 274,548 + HSERO_RS01295 0.30 +3.9 274,590 - HSERO_RS01295 0.32 +3.1 274,723 + HSERO_RS01295 0.39 +2.5 274,743 + HSERO_RS01295 0.40 +2.8 274,782 - HSERO_RS01295 0.42 +3.4 274,787 - HSERO_RS01295 0.42 +3.4 274,795 - HSERO_RS01295 0.42 +3.2 275,164 + HSERO_RS01295 0.61 +3.3 275,321 - HSERO_RS01295 0.69 +3.4 275,584 + HSERO_RS01295 0.82 +3.4 275,584 + HSERO_RS01295 0.82 +3.4 275,819 + +3.4 275,926 + -0.1 275,934 - +1.3 275,990 - -0.7 276,010 - +0.0 276,015 + -1.1 276,085 - +0.3 276,090 + +0.0 276,264 - HSERO_RS01300 0.16 -1.0 276,269 - HSERO_RS01300 0.17 -0.3 276,415 + HSERO_RS01300 0.32 +0.6 276,455 + HSERO_RS01300 0.36 -0.3 276,503 + HSERO_RS01300 0.41 -0.8 276,536 - HSERO_RS01300 0.45 -0.3 276,536 - HSERO_RS01300 0.45 +0.3 276,551 - HSERO_RS01300 0.46 -0.1 276,683 - HSERO_RS01300 0.60 -0.3
Or see this region's nucleotide sequence