Experiment: BG11 with Nalidixic 0.0078125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aas and Synpcc7942_0919 are separated by 48 nucleotides Synpcc7942_0919 and psaK1 are separated by 196 nucleotides psaK1 and Synpcc7942_0921 are separated by 117 nucleotides Synpcc7942_0921 and gltX overlap by 4 nucleotides
Synpcc7942_0918: aas - long-chain-fatty-acid CoA ligase, at 924,079 to 926,028
aas
Synpcc7942_0919: Synpcc7942_0919 - hypothetical protein, at 926,077 to 926,523
_0919
Synpcc7942_0920: psaK1 - photosystem I reaction center, at 926,720 to 926,974
psaK1
Synpcc7942_0921: Synpcc7942_0921 - hypothetical protein, at 927,092 to 927,394
_0921
Synpcc7942_0922: gltX - Glutamate--tRNA ligase, at 927,391 to 928,293
gltX
Position (kb) 926
927 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 925.096 kb on - strand, within aas at 925.121 kb on + strand, within aas at 925.126 kb on + strand, within aas at 925.137 kb on + strand, within aas at 925.147 kb on + strand, within aas at 925.160 kb on + strand, within aas at 925.181 kb on - strand, within aas at 925.182 kb on + strand, within aas at 925.195 kb on + strand, within aas at 925.203 kb on - strand, within aas at 925.223 kb on - strand, within aas at 925.273 kb on + strand, within aas at 925.280 kb on - strand at 925.316 kb on + strand, within aas at 925.323 kb on - strand, within aas at 925.324 kb on - strand, within aas at 925.340 kb on - strand, within aas at 925.342 kb on - strand, within aas at 925.371 kb on + strand, within aas at 925.378 kb on - strand, within aas at 925.385 kb on - strand, within aas at 925.409 kb on - strand, within aas at 925.446 kb on + strand, within aas at 925.473 kb on + strand, within aas at 925.473 kb on + strand, within aas at 925.481 kb on - strand, within aas at 925.481 kb on - strand, within aas at 925.502 kb on + strand, within aas at 925.564 kb on + strand, within aas at 925.573 kb on - strand, within aas at 925.602 kb on + strand, within aas at 925.606 kb on - strand, within aas at 925.611 kb on + strand, within aas at 925.617 kb on + strand, within aas at 925.626 kb on - strand, within aas at 925.635 kb on - strand, within aas at 925.635 kb on - strand, within aas at 925.640 kb on - strand, within aas at 925.647 kb on + strand, within aas at 925.647 kb on + strand, within aas at 925.685 kb on + strand, within aas at 925.685 kb on - strand, within aas at 925.739 kb on + strand, within aas at 925.744 kb on + strand, within aas at 925.762 kb on - strand, within aas at 925.802 kb on - strand, within aas at 925.814 kb on + strand, within aas at 925.822 kb on - strand, within aas at 925.822 kb on - strand, within aas at 925.835 kb on + strand at 925.840 kb on + strand at 925.855 kb on - strand at 925.890 kb on + strand at 925.910 kb on + strand at 926.042 kb on + strand at 926.050 kb on - strand at 926.075 kb on + strand at 926.092 kb on + strand at 926.093 kb on - strand at 926.126 kb on - strand, within Synpcc7942_0919 at 926.186 kb on - strand, within Synpcc7942_0919 at 926.279 kb on - strand, within Synpcc7942_0919 at 926.436 kb on + strand, within Synpcc7942_0919 at 926.500 kb on - strand at 926.526 kb on - strand at 926.672 kb on + strand at 926.699 kb on + strand at 926.704 kb on + strand at 926.709 kb on + strand at 926.801 kb on + strand, within psaK1 at 926.806 kb on + strand, within psaK1 at 926.895 kb on + strand, within psaK1 at 926.933 kb on + strand, within psaK1 at 926.935 kb on + strand, within psaK1 at 927.007 kb on - strand at 927.048 kb on - strand at 927.114 kb on + strand at 927.122 kb on - strand at 927.122 kb on - strand at 927.165 kb on - strand, within Synpcc7942_0921 at 927.177 kb on - strand, within Synpcc7942_0921 at 927.177 kb on - strand, within Synpcc7942_0921 at 927.181 kb on + strand, within Synpcc7942_0921 at 927.201 kb on - strand, within Synpcc7942_0921 at 927.202 kb on + strand, within Synpcc7942_0921 at 927.275 kb on + strand, within Synpcc7942_0921 at 927.275 kb on + strand, within Synpcc7942_0921 at 927.339 kb on - strand, within Synpcc7942_0921 at 927.368 kb on - strand at 927.389 kb on - strand at 927.420 kb on + strand at 927.420 kb on + strand at 927.420 kb on + strand at 927.428 kb on + strand at 927.446 kb on + strand at 927.451 kb on + strand at 927.454 kb on - strand at 927.478 kb on + strand at 927.486 kb on - strand, within gltX at 927.508 kb on - strand, within gltX
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Nalidixic 0.0078125 mg/ml remove 925,096 - aas Synpcc7942_0918 0.52 -1.0 925,121 + aas Synpcc7942_0918 0.53 -0.4 925,126 + aas Synpcc7942_0918 0.54 -0.6 925,137 + aas Synpcc7942_0918 0.54 -1.3 925,147 + aas Synpcc7942_0918 0.55 +0.5 925,160 + aas Synpcc7942_0918 0.55 -0.9 925,181 - aas Synpcc7942_0918 0.57 -0.1 925,182 + aas Synpcc7942_0918 0.57 +0.3 925,195 + aas Synpcc7942_0918 0.57 -0.3 925,203 - aas Synpcc7942_0918 0.58 -1.5 925,223 - aas Synpcc7942_0918 0.59 +0.8 925,273 + aas Synpcc7942_0918 0.61 -1.8 925,280 - +0.8 925,316 + aas Synpcc7942_0918 0.63 -0.7 925,323 - aas Synpcc7942_0918 0.64 -0.5 925,324 - aas Synpcc7942_0918 0.64 -0.3 925,340 - aas Synpcc7942_0918 0.65 -0.7 925,342 - aas Synpcc7942_0918 0.65 -0.1 925,371 + aas Synpcc7942_0918 0.66 -1.1 925,378 - aas Synpcc7942_0918 0.67 -1.8 925,385 - aas Synpcc7942_0918 0.67 -0.2 925,409 - aas Synpcc7942_0918 0.68 -1.3 925,446 + aas Synpcc7942_0918 0.70 -2.8 925,473 + aas Synpcc7942_0918 0.71 -0.9 925,473 + aas Synpcc7942_0918 0.71 -0.6 925,481 - aas Synpcc7942_0918 0.72 -0.2 925,481 - aas Synpcc7942_0918 0.72 -1.2 925,502 + aas Synpcc7942_0918 0.73 +0.5 925,564 + aas Synpcc7942_0918 0.76 -0.3 925,573 - aas Synpcc7942_0918 0.77 -1.3 925,602 + aas Synpcc7942_0918 0.78 +0.3 925,606 - aas Synpcc7942_0918 0.78 -0.2 925,611 + aas Synpcc7942_0918 0.79 +0.8 925,617 + aas Synpcc7942_0918 0.79 -0.4 925,626 - aas Synpcc7942_0918 0.79 -0.7 925,635 - aas Synpcc7942_0918 0.80 -2.4 925,635 - aas Synpcc7942_0918 0.80 -1.1 925,640 - aas Synpcc7942_0918 0.80 -1.8 925,647 + aas Synpcc7942_0918 0.80 -0.1 925,647 + aas Synpcc7942_0918 0.80 -0.8 925,685 + aas Synpcc7942_0918 0.82 +0.2 925,685 - aas Synpcc7942_0918 0.82 -0.7 925,739 + aas Synpcc7942_0918 0.85 -2.4 925,744 + aas Synpcc7942_0918 0.85 -0.5 925,762 - aas Synpcc7942_0918 0.86 +0.1 925,802 - aas Synpcc7942_0918 0.88 +0.3 925,814 + aas Synpcc7942_0918 0.89 +0.0 925,822 - aas Synpcc7942_0918 0.89 -1.0 925,822 - aas Synpcc7942_0918 0.89 -3.0 925,835 + -0.8 925,840 + +0.5 925,855 - -0.7 925,890 + -1.1 925,910 + -0.9 926,042 + -0.3 926,050 - -2.6 926,075 + -0.1 926,092 + -0.3 926,093 - -2.1 926,126 - Synpcc7942_0919 0.11 -1.2 926,186 - Synpcc7942_0919 0.24 -1.5 926,279 - Synpcc7942_0919 0.45 -0.3 926,436 + Synpcc7942_0919 0.80 -1.2 926,500 - +0.3 926,526 - +0.2 926,672 + +0.5 926,699 + +1.0 926,704 + -0.3 926,709 + -0.7 926,801 + psaK1 Synpcc7942_0920 0.32 -0.4 926,806 + psaK1 Synpcc7942_0920 0.34 +1.7 926,895 + psaK1 Synpcc7942_0920 0.69 -0.2 926,933 + psaK1 Synpcc7942_0920 0.84 -0.0 926,935 + psaK1 Synpcc7942_0920 0.84 +0.0 927,007 - +0.1 927,048 - +0.7 927,114 + +0.6 927,122 - -0.5 927,122 - +0.3 927,165 - Synpcc7942_0921 0.24 -0.1 927,177 - Synpcc7942_0921 0.28 -2.0 927,177 - Synpcc7942_0921 0.28 +0.6 927,181 + Synpcc7942_0921 0.29 -0.8 927,201 - Synpcc7942_0921 0.36 +0.7 927,202 + Synpcc7942_0921 0.36 -0.6 927,275 + Synpcc7942_0921 0.60 -1.1 927,275 + Synpcc7942_0921 0.60 -0.6 927,339 - Synpcc7942_0921 0.82 -0.5 927,368 - -0.4 927,389 - +0.4 927,420 + -0.1 927,420 + +0.5 927,420 + -0.1 927,428 + -0.2 927,446 + +0.2 927,451 + +0.4 927,454 - -0.5 927,478 + +1.0 927,486 - gltX Synpcc7942_0922 0.11 -0.1 927,508 - gltX Synpcc7942_0922 0.13 +0.4
Or see this region's nucleotide sequence