Experiment: Cesium chloride 0.00078125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1819 and Pf6N2E2_1820 are separated by 18 nucleotides Pf6N2E2_1820 and Pf6N2E2_1821 are separated by 64 nucleotides Pf6N2E2_1821 and Pf6N2E2_1822 are separated by 25 nucleotides
Pf6N2E2_1819 - 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7), at 2,152,551 to 2,153,567
_1819
Pf6N2E2_1820 - hypothetical protein, at 2,153,586 to 2,153,702
_1820
Pf6N2E2_1821 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 2,153,767 to 2,154,276
_1821
Pf6N2E2_1822 - sensor histidine kinase, at 2,154,302 to 2,158,021
_1822
Position (kb)
2153
2154
2155 Strain fitness (log2 ratio)
-1
0
1 at 2152.845 kb on - strand, within Pf6N2E2_1819 at 2152.994 kb on - strand, within Pf6N2E2_1819 at 2152.994 kb on - strand, within Pf6N2E2_1819 at 2153.190 kb on + strand, within Pf6N2E2_1819 at 2153.191 kb on - strand, within Pf6N2E2_1819 at 2153.239 kb on + strand, within Pf6N2E2_1819 at 2153.240 kb on - strand, within Pf6N2E2_1819 at 2153.398 kb on + strand, within Pf6N2E2_1819 at 2153.399 kb on - strand, within Pf6N2E2_1819 at 2153.465 kb on - strand, within Pf6N2E2_1819 at 2153.549 kb on + strand at 2153.585 kb on - strand at 2154.134 kb on - strand, within Pf6N2E2_1821 at 2154.199 kb on + strand, within Pf6N2E2_1821 at 2154.299 kb on + strand at 2154.311 kb on - strand at 2154.476 kb on - strand at 2154.533 kb on + strand at 2154.749 kb on + strand, within Pf6N2E2_1822 at 2154.750 kb on - strand, within Pf6N2E2_1822 at 2154.795 kb on + strand, within Pf6N2E2_1822 at 2154.795 kb on + strand, within Pf6N2E2_1822 at 2154.796 kb on - strand, within Pf6N2E2_1822 at 2154.796 kb on - strand, within Pf6N2E2_1822 at 2154.796 kb on - strand, within Pf6N2E2_1822 at 2155.133 kb on - strand, within Pf6N2E2_1822 at 2155.219 kb on + strand, within Pf6N2E2_1822
Per-strain Table
Position Strand Gene LocusTag Fraction Cesium chloride 0.00078125 M remove 2,152,845 - Pf6N2E2_1819 0.29 +0.5 2,152,994 - Pf6N2E2_1819 0.44 -0.5 2,152,994 - Pf6N2E2_1819 0.44 +0.9 2,153,190 + Pf6N2E2_1819 0.63 -0.8 2,153,191 - Pf6N2E2_1819 0.63 -0.9 2,153,239 + Pf6N2E2_1819 0.68 +1.1 2,153,240 - Pf6N2E2_1819 0.68 +0.5 2,153,398 + Pf6N2E2_1819 0.83 -0.2 2,153,399 - Pf6N2E2_1819 0.83 -0.3 2,153,465 - Pf6N2E2_1819 0.90 -1.1 2,153,549 + -1.5 2,153,585 - -0.6 2,154,134 - Pf6N2E2_1821 0.72 -1.4 2,154,199 + Pf6N2E2_1821 0.85 -0.7 2,154,299 + +0.6 2,154,311 - -1.1 2,154,476 - +0.1 2,154,533 + +0.3 2,154,749 + Pf6N2E2_1822 0.12 +1.2 2,154,750 - Pf6N2E2_1822 0.12 -0.9 2,154,795 + Pf6N2E2_1822 0.13 -0.4 2,154,795 + Pf6N2E2_1822 0.13 +0.8 2,154,796 - Pf6N2E2_1822 0.13 +0.6 2,154,796 - Pf6N2E2_1822 0.13 -0.4 2,154,796 - Pf6N2E2_1822 0.13 +0.5 2,155,133 - Pf6N2E2_1822 0.22 -0.0 2,155,219 + Pf6N2E2_1822 0.25 +0.2
Or see this region's nucleotide sequence