Experiment: Cesium chloride 0.00078125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1609 and Pf6N2E2_1610 overlap by 4 nucleotides Pf6N2E2_1610 and Pf6N2E2_1611 overlap by 7 nucleotides Pf6N2E2_1611 and Pf6N2E2_1612 overlap by 4 nucleotides Pf6N2E2_1612 and Pf6N2E2_1613 are separated by 186 nucleotides
Pf6N2E2_1609 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 1,883,343 to 1,884,194
_1609
Pf6N2E2_1610 - Phosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 1,884,191 to 1,884,988
_1610
Pf6N2E2_1611 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 1,884,982 to 1,885,809
_1611
Pf6N2E2_1612 - Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1), at 1,885,806 to 1,886,573
_1612
Pf6N2E2_1613 - short-chain dehydrogenase/reductase, at 1,886,760 to 1,887,503
_1613
Position (kb)
1884
1885
1886 Strain fitness (log2 ratio)
-1
0
1
2 at 1883.988 kb on + strand, within Pf6N2E2_1609 at 1883.988 kb on + strand, within Pf6N2E2_1609 at 1883.988 kb on + strand, within Pf6N2E2_1609 at 1883.988 kb on + strand, within Pf6N2E2_1609 at 1883.998 kb on - strand, within Pf6N2E2_1609 at 1883.998 kb on - strand, within Pf6N2E2_1609 at 1884.047 kb on - strand, within Pf6N2E2_1609 at 1884.047 kb on - strand, within Pf6N2E2_1609 at 1884.147 kb on + strand at 1884.147 kb on + strand at 1884.147 kb on + strand at 1884.147 kb on + strand at 1884.147 kb on + strand at 1884.148 kb on - strand at 1884.148 kb on - strand at 1884.148 kb on - strand at 1884.148 kb on - strand at 1884.917 kb on + strand at 1884.933 kb on + strand at 1884.934 kb on - strand at 1884.934 kb on - strand at 1884.934 kb on - strand at 1885.136 kb on + strand, within Pf6N2E2_1611 at 1885.458 kb on + strand, within Pf6N2E2_1611 at 1885.458 kb on + strand, within Pf6N2E2_1611 at 1885.459 kb on - strand, within Pf6N2E2_1611 at 1885.498 kb on + strand, within Pf6N2E2_1611 at 1885.619 kb on - strand, within Pf6N2E2_1611 at 1885.932 kb on + strand, within Pf6N2E2_1612 at 1885.933 kb on - strand, within Pf6N2E2_1612 at 1886.057 kb on - strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.368 kb on - strand, within Pf6N2E2_1612 at 1886.368 kb on - strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.609 kb on - strand at 1886.698 kb on + strand at 1886.745 kb on + strand at 1886.766 kb on - strand at 1886.787 kb on - strand at 1886.791 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cesium chloride 0.00078125 M remove 1,883,988 + Pf6N2E2_1609 0.76 -0.3 1,883,988 + Pf6N2E2_1609 0.76 -0.0 1,883,988 + Pf6N2E2_1609 0.76 -0.6 1,883,988 + Pf6N2E2_1609 0.76 -0.1 1,883,998 - Pf6N2E2_1609 0.77 -0.7 1,883,998 - Pf6N2E2_1609 0.77 -0.7 1,884,047 - Pf6N2E2_1609 0.83 +0.0 1,884,047 - Pf6N2E2_1609 0.83 -0.6 1,884,147 + +0.5 1,884,147 + -0.3 1,884,147 + +1.0 1,884,147 + +1.3 1,884,147 + -0.4 1,884,148 - +0.6 1,884,148 - +0.4 1,884,148 - -0.1 1,884,148 - -0.2 1,884,917 + +0.9 1,884,933 + -0.2 1,884,934 - +0.1 1,884,934 - +0.5 1,884,934 - +0.0 1,885,136 + Pf6N2E2_1611 0.19 -0.8 1,885,458 + Pf6N2E2_1611 0.57 +0.1 1,885,458 + Pf6N2E2_1611 0.57 +0.8 1,885,459 - Pf6N2E2_1611 0.58 -0.2 1,885,498 + Pf6N2E2_1611 0.62 -0.4 1,885,619 - Pf6N2E2_1611 0.77 -1.7 1,885,932 + Pf6N2E2_1612 0.16 -0.4 1,885,933 - Pf6N2E2_1612 0.17 -1.2 1,886,057 - Pf6N2E2_1612 0.33 +0.0 1,886,367 + Pf6N2E2_1612 0.73 -1.1 1,886,367 + Pf6N2E2_1612 0.73 +2.7 1,886,367 + Pf6N2E2_1612 0.73 +0.1 1,886,367 + Pf6N2E2_1612 0.73 -0.3 1,886,368 - Pf6N2E2_1612 0.73 -0.8 1,886,368 - Pf6N2E2_1612 0.73 -0.3 1,886,451 + Pf6N2E2_1612 0.84 -1.5 1,886,451 + Pf6N2E2_1612 0.84 -1.1 1,886,451 + Pf6N2E2_1612 0.84 +0.6 1,886,451 + Pf6N2E2_1612 0.84 +0.1 1,886,609 - -0.8 1,886,698 + -0.2 1,886,745 + +0.1 1,886,766 - -0.3 1,886,787 - -0.2 1,886,791 + +0.6
Or see this region's nucleotide sequence