Experiment: Cesium chloride 0.00078125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1496 and Pf6N2E2_1497 overlap by 4 nucleotides Pf6N2E2_1497 and Pf6N2E2_1498 overlap by 4 nucleotides Pf6N2E2_1498 and Pf6N2E2_1499 are separated by 52 nucleotides
Pf6N2E2_1496 - Aspartate aminotransferase (EC 2.6.1.1), at 1,749,482 to 1,750,693
_1496
Pf6N2E2_1497 - D-2-hydroxyglutarate dehydrogenase, at 1,750,690 to 1,752,114
_1497
Pf6N2E2_1498 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 1,752,111 to 1,752,962
_1498
Pf6N2E2_1499 - ABC transporter amino acid-binding protein, at 1,753,015 to 1,753,857
_1499
Position (kb)
1750
1751
1752
1753 Strain fitness (log2 ratio)
-1
0
1 at 1749.798 kb on + strand, within Pf6N2E2_1496 at 1749.969 kb on + strand, within Pf6N2E2_1496 at 1749.969 kb on + strand, within Pf6N2E2_1496 at 1749.970 kb on - strand, within Pf6N2E2_1496 at 1750.063 kb on + strand, within Pf6N2E2_1496 at 1750.071 kb on + strand, within Pf6N2E2_1496 at 1750.500 kb on + strand, within Pf6N2E2_1496 at 1750.501 kb on - strand, within Pf6N2E2_1496 at 1750.501 kb on - strand, within Pf6N2E2_1496 at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.684 kb on - strand at 1750.684 kb on - strand at 1750.684 kb on - strand at 1750.805 kb on - strand at 1750.880 kb on - strand, within Pf6N2E2_1497 at 1750.949 kb on + strand, within Pf6N2E2_1497 at 1750.949 kb on + strand, within Pf6N2E2_1497 at 1750.985 kb on + strand, within Pf6N2E2_1497 at 1751.085 kb on + strand, within Pf6N2E2_1497 at 1751.116 kb on - strand, within Pf6N2E2_1497 at 1751.116 kb on - strand, within Pf6N2E2_1497 at 1751.499 kb on + strand, within Pf6N2E2_1497 at 1751.499 kb on + strand, within Pf6N2E2_1497 at 1751.500 kb on - strand, within Pf6N2E2_1497 at 1751.987 kb on - strand at 1752.036 kb on - strand at 1752.146 kb on - strand at 1752.381 kb on + strand, within Pf6N2E2_1498 at 1752.381 kb on + strand, within Pf6N2E2_1498 at 1752.382 kb on - strand, within Pf6N2E2_1498 at 1752.382 kb on - strand, within Pf6N2E2_1498 at 1752.387 kb on + strand, within Pf6N2E2_1498 at 1752.388 kb on - strand, within Pf6N2E2_1498 at 1752.593 kb on - strand, within Pf6N2E2_1498 at 1752.608 kb on - strand, within Pf6N2E2_1498 at 1752.769 kb on + strand, within Pf6N2E2_1498 at 1752.770 kb on - strand, within Pf6N2E2_1498 at 1752.770 kb on - strand, within Pf6N2E2_1498 at 1752.970 kb on + strand at 1752.970 kb on + strand at 1752.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cesium chloride 0.00078125 M remove 1,749,798 + Pf6N2E2_1496 0.26 -1.5 1,749,969 + Pf6N2E2_1496 0.40 -0.2 1,749,969 + Pf6N2E2_1496 0.40 +0.7 1,749,970 - Pf6N2E2_1496 0.40 +0.3 1,750,063 + Pf6N2E2_1496 0.48 +0.2 1,750,071 + Pf6N2E2_1496 0.49 -0.0 1,750,500 + Pf6N2E2_1496 0.84 +1.3 1,750,501 - Pf6N2E2_1496 0.84 -0.4 1,750,501 - Pf6N2E2_1496 0.84 +0.1 1,750,683 + -0.1 1,750,683 + +0.3 1,750,683 + +0.2 1,750,683 + -0.8 1,750,683 + +0.7 1,750,684 - +0.3 1,750,684 - +0.7 1,750,684 - +0.9 1,750,805 - +0.2 1,750,880 - Pf6N2E2_1497 0.13 -0.4 1,750,949 + Pf6N2E2_1497 0.18 +1.1 1,750,949 + Pf6N2E2_1497 0.18 -0.9 1,750,985 + Pf6N2E2_1497 0.21 -0.0 1,751,085 + Pf6N2E2_1497 0.28 +0.3 1,751,116 - Pf6N2E2_1497 0.30 -0.8 1,751,116 - Pf6N2E2_1497 0.30 +0.6 1,751,499 + Pf6N2E2_1497 0.57 -1.1 1,751,499 + Pf6N2E2_1497 0.57 -0.3 1,751,500 - Pf6N2E2_1497 0.57 -0.3 1,751,987 - -0.9 1,752,036 - +0.6 1,752,146 - -0.8 1,752,381 + Pf6N2E2_1498 0.32 +0.7 1,752,381 + Pf6N2E2_1498 0.32 +0.2 1,752,382 - Pf6N2E2_1498 0.32 +0.3 1,752,382 - Pf6N2E2_1498 0.32 -0.3 1,752,387 + Pf6N2E2_1498 0.32 -0.4 1,752,388 - Pf6N2E2_1498 0.33 +0.4 1,752,593 - Pf6N2E2_1498 0.57 +0.4 1,752,608 - Pf6N2E2_1498 0.58 +0.0 1,752,769 + Pf6N2E2_1498 0.77 -1.3 1,752,770 - Pf6N2E2_1498 0.77 -0.4 1,752,770 - Pf6N2E2_1498 0.77 +0.2 1,752,970 + +0.1 1,752,970 + +0.3 1,752,971 - +0.5
Or see this region's nucleotide sequence