Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1497

Experiment: Cesium chloride 0.00078125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1496 and Pf6N2E2_1497 overlap by 4 nucleotidesPf6N2E2_1497 and Pf6N2E2_1498 overlap by 4 nucleotidesPf6N2E2_1498 and Pf6N2E2_1499 are separated by 52 nucleotides Pf6N2E2_1496 - Aspartate aminotransferase (EC 2.6.1.1), at 1,749,482 to 1,750,693 _1496 Pf6N2E2_1497 - D-2-hydroxyglutarate dehydrogenase, at 1,750,690 to 1,752,114 _1497 Pf6N2E2_1498 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 1,752,111 to 1,752,962 _1498 Pf6N2E2_1499 - ABC transporter amino acid-binding protein, at 1,753,015 to 1,753,857 _1499 Position (kb) 1750 1751 1752 1753Strain fitness (log2 ratio) -1 0 1at 1749.798 kb on + strand, within Pf6N2E2_1496at 1749.969 kb on + strand, within Pf6N2E2_1496at 1749.969 kb on + strand, within Pf6N2E2_1496at 1749.970 kb on - strand, within Pf6N2E2_1496at 1750.063 kb on + strand, within Pf6N2E2_1496at 1750.071 kb on + strand, within Pf6N2E2_1496at 1750.500 kb on + strand, within Pf6N2E2_1496at 1750.501 kb on - strand, within Pf6N2E2_1496at 1750.501 kb on - strand, within Pf6N2E2_1496at 1750.683 kb on + strandat 1750.683 kb on + strandat 1750.683 kb on + strandat 1750.683 kb on + strandat 1750.683 kb on + strandat 1750.684 kb on - strandat 1750.684 kb on - strandat 1750.684 kb on - strandat 1750.805 kb on - strandat 1750.880 kb on - strand, within Pf6N2E2_1497at 1750.949 kb on + strand, within Pf6N2E2_1497at 1750.949 kb on + strand, within Pf6N2E2_1497at 1750.985 kb on + strand, within Pf6N2E2_1497at 1751.085 kb on + strand, within Pf6N2E2_1497at 1751.116 kb on - strand, within Pf6N2E2_1497at 1751.116 kb on - strand, within Pf6N2E2_1497at 1751.499 kb on + strand, within Pf6N2E2_1497at 1751.499 kb on + strand, within Pf6N2E2_1497at 1751.500 kb on - strand, within Pf6N2E2_1497at 1751.987 kb on - strandat 1752.036 kb on - strandat 1752.146 kb on - strandat 1752.381 kb on + strand, within Pf6N2E2_1498at 1752.381 kb on + strand, within Pf6N2E2_1498at 1752.382 kb on - strand, within Pf6N2E2_1498at 1752.382 kb on - strand, within Pf6N2E2_1498at 1752.387 kb on + strand, within Pf6N2E2_1498at 1752.388 kb on - strand, within Pf6N2E2_1498at 1752.593 kb on - strand, within Pf6N2E2_1498at 1752.608 kb on - strand, within Pf6N2E2_1498at 1752.769 kb on + strand, within Pf6N2E2_1498at 1752.770 kb on - strand, within Pf6N2E2_1498at 1752.770 kb on - strand, within Pf6N2E2_1498at 1752.970 kb on + strandat 1752.970 kb on + strandat 1752.971 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cesium chloride 0.00078125 M
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1,749,798 + Pf6N2E2_1496 0.26 -1.5
1,749,969 + Pf6N2E2_1496 0.40 -0.2
1,749,969 + Pf6N2E2_1496 0.40 +0.7
1,749,970 - Pf6N2E2_1496 0.40 +0.3
1,750,063 + Pf6N2E2_1496 0.48 +0.2
1,750,071 + Pf6N2E2_1496 0.49 -0.0
1,750,500 + Pf6N2E2_1496 0.84 +1.3
1,750,501 - Pf6N2E2_1496 0.84 -0.4
1,750,501 - Pf6N2E2_1496 0.84 +0.1
1,750,683 + -0.1
1,750,683 + +0.3
1,750,683 + +0.2
1,750,683 + -0.8
1,750,683 + +0.7
1,750,684 - +0.3
1,750,684 - +0.7
1,750,684 - +0.9
1,750,805 - +0.2
1,750,880 - Pf6N2E2_1497 0.13 -0.4
1,750,949 + Pf6N2E2_1497 0.18 +1.1
1,750,949 + Pf6N2E2_1497 0.18 -0.9
1,750,985 + Pf6N2E2_1497 0.21 -0.0
1,751,085 + Pf6N2E2_1497 0.28 +0.3
1,751,116 - Pf6N2E2_1497 0.30 -0.8
1,751,116 - Pf6N2E2_1497 0.30 +0.6
1,751,499 + Pf6N2E2_1497 0.57 -1.1
1,751,499 + Pf6N2E2_1497 0.57 -0.3
1,751,500 - Pf6N2E2_1497 0.57 -0.3
1,751,987 - -0.9
1,752,036 - +0.6
1,752,146 - -0.8
1,752,381 + Pf6N2E2_1498 0.32 +0.7
1,752,381 + Pf6N2E2_1498 0.32 +0.2
1,752,382 - Pf6N2E2_1498 0.32 +0.3
1,752,382 - Pf6N2E2_1498 0.32 -0.3
1,752,387 + Pf6N2E2_1498 0.32 -0.4
1,752,388 - Pf6N2E2_1498 0.33 +0.4
1,752,593 - Pf6N2E2_1498 0.57 +0.4
1,752,608 - Pf6N2E2_1498 0.58 +0.0
1,752,769 + Pf6N2E2_1498 0.77 -1.3
1,752,770 - Pf6N2E2_1498 0.77 -0.4
1,752,770 - Pf6N2E2_1498 0.77 +0.2
1,752,970 + +0.1
1,752,970 + +0.3
1,752,971 - +0.5

Or see this region's nucleotide sequence