Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_00510 and IAI47_00515 are separated by 20 nucleotides IAI47_00515 and IAI47_00520 are separated by 54 nucleotides IAI47_00520 and IAI47_00525 are separated by 240 nucleotides
IAI47_00510: IAI47_00510 - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 115,983 to 118,091
_00510
IAI47_00515: IAI47_00515 - DNA-directed RNA polymerase subunit omega, at 118,112 to 118,387
_00515
IAI47_00520: IAI47_00520 - guanylate kinase, at 118,442 to 119,065
_00520
IAI47_00525: IAI47_00525 - NAD-dependent DNA ligase LigB, at 119,306 to 121,057
_00525
Position (kb) 118
119
120 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 118.088 kb on - strand at 118.088 kb on - strand at 118.088 kb on - strand at 118.197 kb on - strand, within IAI47_00515 at 118.250 kb on - strand, within IAI47_00515 at 118.250 kb on - strand, within IAI47_00515 at 118.250 kb on - strand, within IAI47_00515 at 118.256 kb on - strand, within IAI47_00515 at 118.388 kb on - strand at 118.388 kb on - strand at 118.388 kb on - strand at 118.388 kb on - strand at 118.388 kb on - strand at 118.388 kb on - strand at 118.388 kb on - strand at 118.388 kb on - strand at 118.397 kb on - strand at 118.397 kb on - strand at 118.397 kb on - strand at 118.429 kb on - strand at 118.429 kb on - strand at 118.429 kb on - strand at 118.429 kb on - strand at 118.440 kb on - strand at 118.440 kb on - strand at 119.111 kb on - strand at 119.111 kb on - strand at 119.111 kb on - strand at 119.113 kb on - strand at 119.113 kb on - strand at 119.113 kb on - strand at 119.113 kb on - strand at 119.113 kb on - strand at 119.113 kb on - strand at 119.113 kb on - strand at 119.201 kb on + strand at 119.201 kb on + strand at 119.201 kb on + strand at 119.201 kb on + strand at 119.202 kb on - strand at 119.202 kb on - strand at 119.217 kb on - strand at 119.262 kb on + strand at 119.262 kb on + strand at 119.262 kb on + strand at 119.263 kb on - strand at 119.263 kb on - strand at 119.305 kb on + strand at 119.305 kb on + strand at 119.305 kb on + strand at 119.305 kb on + strand at 119.306 kb on - strand at 119.306 kb on - strand at 119.306 kb on - strand at 119.306 kb on - strand at 119.456 kb on + strand at 119.456 kb on + strand at 119.456 kb on + strand at 119.456 kb on + strand at 119.456 kb on + strand at 119.456 kb on + strand at 119.456 kb on + strand at 119.457 kb on - strand at 119.457 kb on - strand at 119.457 kb on - strand at 119.459 kb on + strand at 119.459 kb on + strand at 119.459 kb on + strand at 119.459 kb on + strand at 119.460 kb on - strand at 119.460 kb on - strand at 119.495 kb on + strand, within IAI47_00525 at 119.495 kb on + strand, within IAI47_00525 at 119.496 kb on - strand, within IAI47_00525 at 119.496 kb on - strand, within IAI47_00525 at 119.521 kb on + strand, within IAI47_00525 at 119.521 kb on + strand, within IAI47_00525 at 119.521 kb on + strand, within IAI47_00525 at 119.522 kb on - strand, within IAI47_00525 at 119.522 kb on - strand, within IAI47_00525 at 119.522 kb on - strand, within IAI47_00525 at 119.540 kb on - strand, within IAI47_00525 at 119.558 kb on + strand, within IAI47_00525 at 119.558 kb on + strand, within IAI47_00525 at 119.558 kb on + strand, within IAI47_00525 at 119.558 kb on + strand, within IAI47_00525 at 119.559 kb on - strand, within IAI47_00525 at 119.559 kb on - strand, within IAI47_00525 at 119.559 kb on - strand, within IAI47_00525 at 119.559 kb on - strand, within IAI47_00525 at 119.559 kb on - strand, within IAI47_00525 at 119.559 kb on - strand, within IAI47_00525 at 119.608 kb on + strand, within IAI47_00525 at 119.609 kb on - strand, within IAI47_00525 at 119.648 kb on + strand, within IAI47_00525 at 119.649 kb on - strand, within IAI47_00525 at 119.649 kb on - strand, within IAI47_00525 at 119.649 kb on - strand, within IAI47_00525 at 119.717 kb on + strand, within IAI47_00525 at 119.717 kb on + strand, within IAI47_00525 at 119.717 kb on + strand, within IAI47_00525 at 119.745 kb on + strand, within IAI47_00525 at 119.745 kb on + strand, within IAI47_00525 at 119.746 kb on - strand, within IAI47_00525 at 119.746 kb on - strand, within IAI47_00525 at 119.746 kb on - strand, within IAI47_00525 at 119.818 kb on + strand, within IAI47_00525 at 119.819 kb on - strand, within IAI47_00525 at 119.844 kb on + strand, within IAI47_00525 at 119.845 kb on - strand, within IAI47_00525 at 119.845 kb on - strand, within IAI47_00525
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 118,088 - +3.3 118,088 - -0.2 118,088 - +0.1 118,197 - IAI47_00515 0.31 +4.7 118,250 - IAI47_00515 0.50 -0.0 118,250 - IAI47_00515 0.50 +2.4 118,250 - IAI47_00515 0.50 +0.4 118,256 - IAI47_00515 0.52 -2.4 118,388 - -0.1 118,388 - +2.3 118,388 - -2.6 118,388 - -2.4 118,388 - +1.0 118,388 - +1.1 118,388 - +1.9 118,388 - -1.6 118,397 - -0.4 118,397 - -0.6 118,397 - +0.8 118,429 - -0.3 118,429 - -1.9 118,429 - +0.5 118,429 - -1.6 118,440 - +1.4 118,440 - -0.4 119,111 - -1.4 119,111 - -0.4 119,111 - -1.9 119,113 - +0.1 119,113 - -0.0 119,113 - -1.2 119,113 - +2.4 119,113 - +1.2 119,113 - +0.3 119,113 - +0.6 119,201 + -0.6 119,201 + +1.6 119,201 + -0.2 119,201 + -1.2 119,202 - -0.4 119,202 - -0.9 119,217 - -1.8 119,262 + +1.0 119,262 + -0.9 119,262 + -1.3 119,263 - -0.6 119,263 - +0.5 119,305 + +0.0 119,305 + -0.4 119,305 + +1.9 119,305 + +0.6 119,306 - -0.2 119,306 - -0.4 119,306 - +1.0 119,306 - -0.2 119,456 + -1.0 119,456 + +0.5 119,456 + -2.0 119,456 + +1.1 119,456 + +0.7 119,456 + -1.2 119,456 + -0.4 119,457 - -1.7 119,457 - -0.4 119,457 - +0.8 119,459 + +1.3 119,459 + +0.4 119,459 + +1.0 119,459 + -1.2 119,460 - +0.0 119,460 - -0.8 119,495 + IAI47_00525 0.11 +0.4 119,495 + IAI47_00525 0.11 +2.2 119,496 - IAI47_00525 0.11 -0.8 119,496 - IAI47_00525 0.11 -3.2 119,521 + IAI47_00525 0.12 -0.1 119,521 + IAI47_00525 0.12 +0.9 119,521 + IAI47_00525 0.12 -2.4 119,522 - IAI47_00525 0.12 -0.8 119,522 - IAI47_00525 0.12 +1.8 119,522 - IAI47_00525 0.12 -0.6 119,540 - IAI47_00525 0.13 -0.3 119,558 + IAI47_00525 0.14 +1.8 119,558 + IAI47_00525 0.14 -0.9 119,558 + IAI47_00525 0.14 +1.7 119,558 + IAI47_00525 0.14 +2.1 119,559 - IAI47_00525 0.14 +1.4 119,559 - IAI47_00525 0.14 -1.4 119,559 - IAI47_00525 0.14 +1.1 119,559 - IAI47_00525 0.14 +0.3 119,559 - IAI47_00525 0.14 -0.0 119,559 - IAI47_00525 0.14 +0.1 119,608 + IAI47_00525 0.17 +1.6 119,609 - IAI47_00525 0.17 -2.8 119,648 + IAI47_00525 0.20 +2.0 119,649 - IAI47_00525 0.20 +1.1 119,649 - IAI47_00525 0.20 +1.1 119,649 - IAI47_00525 0.20 -0.3 119,717 + IAI47_00525 0.23 -3.9 119,717 + IAI47_00525 0.23 +0.7 119,717 + IAI47_00525 0.23 -0.4 119,745 + IAI47_00525 0.25 -0.4 119,745 + IAI47_00525 0.25 +3.4 119,746 - IAI47_00525 0.25 +3.3 119,746 - IAI47_00525 0.25 -0.2 119,746 - IAI47_00525 0.25 -2.0 119,818 + IAI47_00525 0.29 +1.1 119,819 - IAI47_00525 0.29 +0.0 119,844 + IAI47_00525 0.31 +3.0 119,845 - IAI47_00525 0.31 -0.6 119,845 - IAI47_00525 0.31 -1.8
Or see this region's nucleotide sequence