Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS14385 and HSERO_RS14390 are separated by 391 nucleotides HSERO_RS14390 and HSERO_RS14395 are separated by 678 nucleotides
HSERO_RS14385: HSERO_RS14385 - FeMo cofactor biosynthesis protein NifB, at 3,255,986 to 3,257,563
_RS14385
HSERO_RS14390: HSERO_RS14390 - ATPase AAA, at 3,257,955 to 3,259,574
_RS14390
HSERO_RS14395: HSERO_RS14395 - oxygen-binding protein, at 3,260,253 to 3,260,699
_RS14395
Position (kb)
3257
3258
3259
3260 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3257.030 kb on - strand, within HSERO_RS14385 at 3257.040 kb on + strand, within HSERO_RS14385 at 3257.094 kb on - strand, within HSERO_RS14385 at 3257.177 kb on - strand, within HSERO_RS14385 at 3257.368 kb on + strand at 3257.434 kb on - strand at 3257.491 kb on - strand at 3257.594 kb on + strand at 3257.682 kb on - strand at 3257.734 kb on + strand at 3257.792 kb on - strand at 3257.804 kb on - strand at 3257.813 kb on + strand at 3258.015 kb on + strand at 3258.047 kb on - strand at 3258.093 kb on + strand at 3258.364 kb on - strand, within HSERO_RS14390 at 3258.365 kb on - strand, within HSERO_RS14390 at 3258.446 kb on - strand, within HSERO_RS14390 at 3258.504 kb on - strand, within HSERO_RS14390 at 3258.504 kb on - strand, within HSERO_RS14390 at 3258.516 kb on + strand, within HSERO_RS14390 at 3258.543 kb on - strand, within HSERO_RS14390 at 3258.737 kb on - strand, within HSERO_RS14390 at 3258.985 kb on + strand, within HSERO_RS14390 at 3259.118 kb on + strand, within HSERO_RS14390 at 3259.200 kb on - strand, within HSERO_RS14390 at 3259.200 kb on - strand, within HSERO_RS14390 at 3259.249 kb on - strand, within HSERO_RS14390 at 3259.303 kb on + strand, within HSERO_RS14390 at 3259.486 kb on + strand at 3259.486 kb on + strand at 3259.486 kb on + strand at 3259.494 kb on - strand at 3259.494 kb on - strand at 3259.579 kb on - strand at 3259.630 kb on - strand at 3259.636 kb on + strand at 3259.743 kb on - strand at 3260.017 kb on - strand at 3260.017 kb on - strand at 3260.191 kb on - strand at 3260.191 kb on - strand at 3260.301 kb on - strand, within HSERO_RS14395 at 3260.381 kb on + strand, within HSERO_RS14395
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 3,257,030 - HSERO_RS14385 0.66 -3.7 3,257,040 + HSERO_RS14385 0.67 -3.6 3,257,094 - HSERO_RS14385 0.70 -2.5 3,257,177 - HSERO_RS14385 0.75 -3.4 3,257,368 + -1.5 3,257,434 - -4.0 3,257,491 - -4.2 3,257,594 + -3.1 3,257,682 - -3.6 3,257,734 + +0.5 3,257,792 - -1.1 3,257,804 - -0.4 3,257,813 + -0.2 3,258,015 + -3.1 3,258,047 - -2.8 3,258,093 + -4.9 3,258,364 - HSERO_RS14390 0.25 -3.2 3,258,365 - HSERO_RS14390 0.25 -2.7 3,258,446 - HSERO_RS14390 0.30 -4.1 3,258,504 - HSERO_RS14390 0.34 -2.8 3,258,504 - HSERO_RS14390 0.34 -3.2 3,258,516 + HSERO_RS14390 0.35 -1.6 3,258,543 - HSERO_RS14390 0.36 -3.6 3,258,737 - HSERO_RS14390 0.48 -2.2 3,258,985 + HSERO_RS14390 0.64 -4.1 3,259,118 + HSERO_RS14390 0.72 -3.1 3,259,200 - HSERO_RS14390 0.77 -2.4 3,259,200 - HSERO_RS14390 0.77 -3.7 3,259,249 - HSERO_RS14390 0.80 -3.9 3,259,303 + HSERO_RS14390 0.83 -3.5 3,259,486 + -2.9 3,259,486 + -2.5 3,259,486 + -3.3 3,259,494 - -5.5 3,259,494 - -3.3 3,259,579 - -3.6 3,259,630 - +0.1 3,259,636 + -2.7 3,259,743 - -1.0 3,260,017 - -0.5 3,260,017 - +0.8 3,260,191 - +0.6 3,260,191 - +1.4 3,260,301 - HSERO_RS14395 0.11 +0.8 3,260,381 + HSERO_RS14395 0.29 +0.4
Or see this region's nucleotide sequence