Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_14025 and IAI47_14030 are separated by 8 nucleotides IAI47_14030 and IAI47_14035 are separated by 10 nucleotides
IAI47_14025: IAI47_14025 - peptidoglycan DD-transpeptidase MrdA, at 2,963,188 to 2,965,092
_14025
IAI47_14030: IAI47_14030 - peptidoglycan glycosyltransferase MrdB, at 2,965,101 to 2,966,213
_14030
IAI47_14035: IAI47_14035 - endolytic peptidoglycan transglycosylase RlpA, at 2,966,224 to 2,967,321
_14035
Position (kb)
2965
2966
2967 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2965.023 kb on + strand at 2965.023 kb on + strand at 2965.023 kb on + strand at 2965.023 kb on + strand at 2965.023 kb on + strand at 2965.023 kb on + strand at 2965.023 kb on + strand at 2965.023 kb on + strand at 2965.023 kb on + strand at 2965.023 kb on + strand at 2965.035 kb on + strand at 2965.035 kb on + strand at 2965.041 kb on + strand at 2965.041 kb on + strand at 2965.041 kb on + strand at 2965.041 kb on + strand at 2965.090 kb on + strand at 2966.201 kb on + strand at 2966.201 kb on + strand at 2966.201 kb on + strand at 2966.201 kb on + strand at 2966.212 kb on - strand at 2966.212 kb on - strand at 2966.229 kb on + strand at 2966.230 kb on - strand at 2966.277 kb on + strand at 2966.277 kb on + strand at 2966.278 kb on - strand at 2966.278 kb on - strand at 2966.298 kb on + strand at 2966.299 kb on - strand at 2966.299 kb on - strand at 2966.299 kb on - strand at 2966.321 kb on - strand at 2966.321 kb on - strand at 2966.362 kb on + strand, within IAI47_14035 at 2966.362 kb on + strand, within IAI47_14035 at 2966.362 kb on + strand, within IAI47_14035 at 2966.362 kb on + strand, within IAI47_14035 at 2966.363 kb on - strand, within IAI47_14035 at 2966.363 kb on - strand, within IAI47_14035 at 2966.363 kb on - strand, within IAI47_14035 at 2966.363 kb on - strand, within IAI47_14035 at 2966.363 kb on - strand, within IAI47_14035 at 2966.395 kb on + strand, within IAI47_14035 at 2966.395 kb on + strand, within IAI47_14035 at 2966.395 kb on + strand, within IAI47_14035 at 2966.396 kb on - strand, within IAI47_14035 at 2966.396 kb on - strand, within IAI47_14035 at 2966.396 kb on - strand, within IAI47_14035 at 2966.443 kb on + strand, within IAI47_14035 at 2966.443 kb on + strand, within IAI47_14035 at 2966.443 kb on + strand, within IAI47_14035 at 2966.471 kb on - strand, within IAI47_14035 at 2966.471 kb on - strand, within IAI47_14035 at 2966.515 kb on + strand, within IAI47_14035 at 2966.515 kb on + strand, within IAI47_14035 at 2966.515 kb on + strand, within IAI47_14035 at 2966.516 kb on - strand, within IAI47_14035 at 2966.516 kb on - strand, within IAI47_14035 at 2966.516 kb on - strand, within IAI47_14035 at 2966.547 kb on + strand, within IAI47_14035 at 2966.547 kb on + strand, within IAI47_14035 at 2966.547 kb on + strand, within IAI47_14035 at 2966.566 kb on + strand, within IAI47_14035 at 2966.566 kb on + strand, within IAI47_14035 at 2966.566 kb on + strand, within IAI47_14035 at 2966.566 kb on + strand, within IAI47_14035 at 2966.567 kb on - strand, within IAI47_14035 at 2966.567 kb on - strand, within IAI47_14035 at 2966.567 kb on - strand, within IAI47_14035 at 2966.567 kb on - strand, within IAI47_14035 at 2966.567 kb on - strand, within IAI47_14035 at 2966.567 kb on - strand, within IAI47_14035 at 2966.567 kb on - strand, within IAI47_14035 at 2966.567 kb on - strand, within IAI47_14035 at 2966.567 kb on - strand, within IAI47_14035 at 2966.567 kb on - strand, within IAI47_14035 at 2966.632 kb on + strand, within IAI47_14035 at 2966.633 kb on - strand, within IAI47_14035 at 2966.633 kb on - strand, within IAI47_14035 at 2966.685 kb on + strand, within IAI47_14035 at 2966.685 kb on + strand, within IAI47_14035 at 2966.716 kb on + strand, within IAI47_14035 at 2966.716 kb on + strand, within IAI47_14035 at 2966.716 kb on + strand, within IAI47_14035 at 2966.717 kb on - strand, within IAI47_14035 at 2966.717 kb on - strand, within IAI47_14035 at 2966.717 kb on - strand, within IAI47_14035 at 2966.788 kb on + strand, within IAI47_14035 at 2966.788 kb on + strand, within IAI47_14035 at 2966.788 kb on + strand, within IAI47_14035 at 2966.788 kb on + strand, within IAI47_14035 at 2966.788 kb on + strand, within IAI47_14035 at 2966.788 kb on + strand, within IAI47_14035 at 2966.789 kb on - strand, within IAI47_14035 at 2966.789 kb on - strand, within IAI47_14035 at 2966.789 kb on - strand, within IAI47_14035 at 2966.789 kb on - strand, within IAI47_14035 at 2966.789 kb on - strand, within IAI47_14035 at 2966.789 kb on - strand, within IAI47_14035 at 2966.978 kb on + strand, within IAI47_14035 at 2966.978 kb on + strand, within IAI47_14035 at 2966.979 kb on - strand, within IAI47_14035 at 2966.988 kb on + strand, within IAI47_14035 at 2966.988 kb on + strand, within IAI47_14035 at 2966.988 kb on + strand, within IAI47_14035 at 2966.988 kb on + strand, within IAI47_14035 at 2966.988 kb on + strand, within IAI47_14035 at 2967.097 kb on + strand, within IAI47_14035 at 2967.097 kb on + strand, within IAI47_14035 at 2967.097 kb on + strand, within IAI47_14035 at 2967.098 kb on - strand, within IAI47_14035 at 2967.098 kb on - strand, within IAI47_14035 at 2967.098 kb on - strand, within IAI47_14035 at 2967.098 kb on - strand, within IAI47_14035 at 2967.098 kb on - strand, within IAI47_14035 at 2967.098 kb on - strand, within IAI47_14035 at 2967.098 kb on - strand, within IAI47_14035 at 2967.098 kb on - strand, within IAI47_14035 at 2967.098 kb on - strand, within IAI47_14035 at 2967.098 kb on - strand, within IAI47_14035 at 2967.098 kb on - strand, within IAI47_14035 at 2967.169 kb on + strand, within IAI47_14035 at 2967.211 kb on + strand, within IAI47_14035 at 2967.212 kb on - strand at 2967.212 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,965,023 + +0.1 2,965,023 + -0.0 2,965,023 + +1.4 2,965,023 + -0.2 2,965,023 + +1.1 2,965,023 + +0.3 2,965,023 + +2.0 2,965,023 + +0.8 2,965,023 + +1.3 2,965,023 + +0.4 2,965,035 + +1.2 2,965,035 + +0.1 2,965,041 + -0.6 2,965,041 + +0.4 2,965,041 + -1.3 2,965,041 + -0.5 2,965,090 + +2.5 2,966,201 + -1.3 2,966,201 + -0.5 2,966,201 + -0.1 2,966,201 + -1.1 2,966,212 - -0.2 2,966,212 - -0.1 2,966,229 + -0.5 2,966,230 - -0.9 2,966,277 + +1.0 2,966,277 + +0.7 2,966,278 - -0.1 2,966,278 - -1.5 2,966,298 + +0.8 2,966,299 - -0.9 2,966,299 - -0.7 2,966,299 - -0.4 2,966,321 - -1.4 2,966,321 - +0.6 2,966,362 + IAI47_14035 0.13 -0.1 2,966,362 + IAI47_14035 0.13 +1.0 2,966,362 + IAI47_14035 0.13 -0.2 2,966,362 + IAI47_14035 0.13 +1.2 2,966,363 - IAI47_14035 0.13 -0.2 2,966,363 - IAI47_14035 0.13 -1.6 2,966,363 - IAI47_14035 0.13 -0.2 2,966,363 - IAI47_14035 0.13 -0.1 2,966,363 - IAI47_14035 0.13 -2.9 2,966,395 + IAI47_14035 0.16 -0.3 2,966,395 + IAI47_14035 0.16 -0.6 2,966,395 + IAI47_14035 0.16 +0.4 2,966,396 - IAI47_14035 0.16 -0.1 2,966,396 - IAI47_14035 0.16 -1.0 2,966,396 - IAI47_14035 0.16 +0.6 2,966,443 + IAI47_14035 0.20 +0.6 2,966,443 + IAI47_14035 0.20 +0.6 2,966,443 + IAI47_14035 0.20 +1.1 2,966,471 - IAI47_14035 0.22 +0.7 2,966,471 - IAI47_14035 0.22 +0.8 2,966,515 + IAI47_14035 0.27 +0.3 2,966,515 + IAI47_14035 0.27 +0.4 2,966,515 + IAI47_14035 0.27 -0.0 2,966,516 - IAI47_14035 0.27 +0.6 2,966,516 - IAI47_14035 0.27 -2.1 2,966,516 - IAI47_14035 0.27 +0.6 2,966,547 + IAI47_14035 0.29 +0.0 2,966,547 + IAI47_14035 0.29 +0.6 2,966,547 + IAI47_14035 0.29 +1.4 2,966,566 + IAI47_14035 0.31 +0.4 2,966,566 + IAI47_14035 0.31 +1.4 2,966,566 + IAI47_14035 0.31 -0.1 2,966,566 + IAI47_14035 0.31 +2.5 2,966,567 - IAI47_14035 0.31 +0.7 2,966,567 - IAI47_14035 0.31 +0.9 2,966,567 - IAI47_14035 0.31 +1.4 2,966,567 - IAI47_14035 0.31 -0.5 2,966,567 - IAI47_14035 0.31 +0.1 2,966,567 - IAI47_14035 0.31 -0.3 2,966,567 - IAI47_14035 0.31 -0.0 2,966,567 - IAI47_14035 0.31 +0.8 2,966,567 - IAI47_14035 0.31 -1.6 2,966,567 - IAI47_14035 0.31 -0.6 2,966,632 + IAI47_14035 0.37 -1.3 2,966,633 - IAI47_14035 0.37 -0.2 2,966,633 - IAI47_14035 0.37 -0.5 2,966,685 + IAI47_14035 0.42 +1.2 2,966,685 + IAI47_14035 0.42 +0.6 2,966,716 + IAI47_14035 0.45 -0.4 2,966,716 + IAI47_14035 0.45 +0.9 2,966,716 + IAI47_14035 0.45 +0.6 2,966,717 - IAI47_14035 0.45 -1.3 2,966,717 - IAI47_14035 0.45 +0.7 2,966,717 - IAI47_14035 0.45 -0.5 2,966,788 + IAI47_14035 0.51 +0.4 2,966,788 + IAI47_14035 0.51 +0.6 2,966,788 + IAI47_14035 0.51 +0.3 2,966,788 + IAI47_14035 0.51 -0.1 2,966,788 + IAI47_14035 0.51 +0.7 2,966,788 + IAI47_14035 0.51 +0.5 2,966,789 - IAI47_14035 0.51 -0.4 2,966,789 - IAI47_14035 0.51 +0.2 2,966,789 - IAI47_14035 0.51 +0.4 2,966,789 - IAI47_14035 0.51 +1.7 2,966,789 - IAI47_14035 0.51 +0.4 2,966,789 - IAI47_14035 0.51 +0.3 2,966,978 + IAI47_14035 0.69 -0.6 2,966,978 + IAI47_14035 0.69 +2.7 2,966,979 - IAI47_14035 0.69 -0.6 2,966,988 + IAI47_14035 0.70 -0.7 2,966,988 + IAI47_14035 0.70 -0.5 2,966,988 + IAI47_14035 0.70 -0.4 2,966,988 + IAI47_14035 0.70 +0.6 2,966,988 + IAI47_14035 0.70 -0.1 2,967,097 + IAI47_14035 0.80 +0.7 2,967,097 + IAI47_14035 0.80 -0.4 2,967,097 + IAI47_14035 0.80 +1.5 2,967,098 - IAI47_14035 0.80 +0.7 2,967,098 - IAI47_14035 0.80 +0.7 2,967,098 - IAI47_14035 0.80 -1.1 2,967,098 - IAI47_14035 0.80 +0.6 2,967,098 - IAI47_14035 0.80 +0.5 2,967,098 - IAI47_14035 0.80 +1.0 2,967,098 - IAI47_14035 0.80 +0.8 2,967,098 - IAI47_14035 0.80 +0.3 2,967,098 - IAI47_14035 0.80 -0.6 2,967,098 - IAI47_14035 0.80 -0.1 2,967,098 - IAI47_14035 0.80 -1.7 2,967,169 + IAI47_14035 0.86 -0.8 2,967,211 + IAI47_14035 0.90 +0.1 2,967,212 - -0.8 2,967,212 - +0.5
Or see this region's nucleotide sequence