Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_10215 and IAI47_10220 are separated by 3 nucleotides IAI47_10220 and IAI47_10225 are separated by 54 nucleotides IAI47_10225 and IAI47_10230 are separated by 143 nucleotides
IAI47_10215: IAI47_10215 - pyrroloquinoline-quinone synthase PqqC, at 2,159,032 to 2,159,787
_10215
IAI47_10220: IAI47_10220 - pyrroloquinoline quinone biosynthesis protein PqqB, at 2,159,791 to 2,160,708
_10220
IAI47_10225: IAI47_10225 - pyrroloquinoline quinone precursor peptide PqqA, at 2,160,763 to 2,160,834
_10225
IAI47_10230: IAI47_10230 - dipeptidase, at 2,160,978 to 2,161,994
_10230
Position (kb)
2160
2161 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2159.792 kb on + strand at 2159.833 kb on + strand at 2159.834 kb on - strand at 2159.834 kb on - strand at 2159.877 kb on + strand at 2159.898 kb on + strand, within IAI47_10220 at 2159.899 kb on - strand, within IAI47_10220 at 2159.899 kb on - strand, within IAI47_10220 at 2159.899 kb on - strand, within IAI47_10220 at 2160.088 kb on + strand, within IAI47_10220 at 2160.088 kb on + strand, within IAI47_10220 at 2160.122 kb on + strand, within IAI47_10220 at 2160.122 kb on + strand, within IAI47_10220 at 2160.123 kb on - strand, within IAI47_10220 at 2160.206 kb on + strand, within IAI47_10220 at 2160.206 kb on + strand, within IAI47_10220 at 2160.206 kb on + strand, within IAI47_10220 at 2160.225 kb on + strand, within IAI47_10220 at 2160.226 kb on - strand, within IAI47_10220 at 2160.304 kb on + strand, within IAI47_10220 at 2160.304 kb on + strand, within IAI47_10220 at 2160.304 kb on + strand, within IAI47_10220 at 2160.304 kb on + strand, within IAI47_10220 at 2160.305 kb on - strand, within IAI47_10220 at 2160.305 kb on - strand, within IAI47_10220 at 2160.378 kb on + strand, within IAI47_10220 at 2160.378 kb on + strand, within IAI47_10220 at 2160.510 kb on + strand, within IAI47_10220 at 2160.511 kb on - strand, within IAI47_10220 at 2160.511 kb on - strand, within IAI47_10220 at 2160.511 kb on - strand, within IAI47_10220 at 2160.511 kb on - strand, within IAI47_10220 at 2160.511 kb on - strand, within IAI47_10220 at 2160.550 kb on + strand, within IAI47_10220 at 2160.550 kb on + strand, within IAI47_10220 at 2160.550 kb on + strand, within IAI47_10220 at 2160.550 kb on + strand, within IAI47_10220 at 2160.551 kb on - strand, within IAI47_10220 at 2160.551 kb on - strand, within IAI47_10220 at 2160.551 kb on - strand, within IAI47_10220 at 2160.551 kb on - strand, within IAI47_10220 at 2160.551 kb on - strand, within IAI47_10220 at 2160.551 kb on - strand, within IAI47_10220 at 2160.582 kb on + strand, within IAI47_10220 at 2160.583 kb on - strand, within IAI47_10220 at 2160.624 kb on + strand at 2160.625 kb on - strand at 2160.625 kb on - strand at 2160.651 kb on + strand at 2160.651 kb on + strand at 2160.651 kb on + strand at 2160.651 kb on + strand at 2160.651 kb on + strand at 2160.652 kb on - strand at 2160.652 kb on - strand at 2160.702 kb on + strand at 2160.708 kb on + strand at 2160.708 kb on + strand at 2160.708 kb on + strand at 2160.708 kb on + strand at 2160.709 kb on - strand at 2160.709 kb on - strand at 2160.765 kb on - strand at 2160.779 kb on + strand, within IAI47_10225 at 2160.779 kb on + strand, within IAI47_10225 at 2160.779 kb on + strand, within IAI47_10225 at 2160.780 kb on - strand, within IAI47_10225 at 2160.780 kb on - strand, within IAI47_10225 at 2160.780 kb on - strand, within IAI47_10225 at 2160.780 kb on - strand, within IAI47_10225 at 2160.780 kb on - strand, within IAI47_10225 at 2160.807 kb on + strand, within IAI47_10225 at 2160.807 kb on + strand, within IAI47_10225 at 2160.808 kb on - strand, within IAI47_10225 at 2160.808 kb on - strand, within IAI47_10225 at 2160.808 kb on - strand, within IAI47_10225 at 2160.808 kb on - strand, within IAI47_10225 at 2160.808 kb on - strand, within IAI47_10225 at 2160.839 kb on + strand at 2160.839 kb on + strand at 2160.839 kb on + strand at 2160.839 kb on + strand at 2160.839 kb on + strand at 2160.840 kb on - strand at 2160.840 kb on - strand at 2160.840 kb on - strand at 2160.860 kb on - strand at 2161.048 kb on + strand at 2161.048 kb on + strand at 2161.048 kb on + strand at 2161.049 kb on - strand at 2161.049 kb on - strand at 2161.164 kb on + strand, within IAI47_10230 at 2161.165 kb on - strand, within IAI47_10230 at 2161.203 kb on + strand, within IAI47_10230 at 2161.227 kb on - strand, within IAI47_10230 at 2161.259 kb on + strand, within IAI47_10230 at 2161.680 kb on - strand, within IAI47_10230 at 2161.680 kb on - strand, within IAI47_10230 at 2161.680 kb on - strand, within IAI47_10230 at 2161.680 kb on - strand, within IAI47_10230 at 2161.682 kb on + strand, within IAI47_10230 at 2161.682 kb on + strand, within IAI47_10230 at 2161.682 kb on + strand, within IAI47_10230 at 2161.682 kb on + strand, within IAI47_10230 at 2161.683 kb on - strand, within IAI47_10230 at 2161.683 kb on - strand, within IAI47_10230 at 2161.817 kb on + strand, within IAI47_10230 at 2161.818 kb on - strand, within IAI47_10230 at 2161.818 kb on - strand, within IAI47_10230 at 2161.818 kb on - strand, within IAI47_10230 at 2161.818 kb on - strand, within IAI47_10230 at 2161.819 kb on + strand, within IAI47_10230 at 2161.819 kb on + strand, within IAI47_10230 at 2161.820 kb on - strand, within IAI47_10230 at 2161.820 kb on - strand, within IAI47_10230 at 2161.820 kb on - strand, within IAI47_10230 at 2161.820 kb on - strand, within IAI47_10230 at 2161.820 kb on - strand, within IAI47_10230 at 2161.820 kb on - strand, within IAI47_10230 at 2161.820 kb on - strand, within IAI47_10230 at 2161.820 kb on - strand, within IAI47_10230 at 2161.820 kb on - strand, within IAI47_10230 at 2161.820 kb on - strand, within IAI47_10230
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,159,792 + +2.2 2,159,833 + +2.0 2,159,834 - +3.1 2,159,834 - +2.1 2,159,877 + -0.4 2,159,898 + IAI47_10220 0.12 -1.0 2,159,899 - IAI47_10220 0.12 +0.4 2,159,899 - IAI47_10220 0.12 +0.4 2,159,899 - IAI47_10220 0.12 +0.5 2,160,088 + IAI47_10220 0.32 +0.4 2,160,088 + IAI47_10220 0.32 -1.5 2,160,122 + IAI47_10220 0.36 +0.9 2,160,122 + IAI47_10220 0.36 +0.6 2,160,123 - IAI47_10220 0.36 +0.8 2,160,206 + IAI47_10220 0.45 +0.6 2,160,206 + IAI47_10220 0.45 +1.2 2,160,206 + IAI47_10220 0.45 +1.4 2,160,225 + IAI47_10220 0.47 -1.2 2,160,226 - IAI47_10220 0.47 +0.4 2,160,304 + IAI47_10220 0.56 -0.5 2,160,304 + IAI47_10220 0.56 +1.3 2,160,304 + IAI47_10220 0.56 +0.0 2,160,304 + IAI47_10220 0.56 +1.2 2,160,305 - IAI47_10220 0.56 +0.8 2,160,305 - IAI47_10220 0.56 +1.5 2,160,378 + IAI47_10220 0.64 -0.3 2,160,378 + IAI47_10220 0.64 +2.0 2,160,510 + IAI47_10220 0.78 -0.4 2,160,511 - IAI47_10220 0.78 +0.5 2,160,511 - IAI47_10220 0.78 +0.8 2,160,511 - IAI47_10220 0.78 +1.8 2,160,511 - IAI47_10220 0.78 +1.2 2,160,511 - IAI47_10220 0.78 -0.7 2,160,550 + IAI47_10220 0.83 -0.1 2,160,550 + IAI47_10220 0.83 +0.6 2,160,550 + IAI47_10220 0.83 +0.9 2,160,550 + IAI47_10220 0.83 +0.2 2,160,551 - IAI47_10220 0.83 +1.6 2,160,551 - IAI47_10220 0.83 -0.4 2,160,551 - IAI47_10220 0.83 +0.0 2,160,551 - IAI47_10220 0.83 +1.0 2,160,551 - IAI47_10220 0.83 -0.1 2,160,551 - IAI47_10220 0.83 +0.6 2,160,582 + IAI47_10220 0.86 +0.9 2,160,583 - IAI47_10220 0.86 +2.2 2,160,624 + +0.3 2,160,625 - +0.4 2,160,625 - +0.2 2,160,651 + -0.2 2,160,651 + +0.1 2,160,651 + +1.4 2,160,651 + +0.4 2,160,651 + +0.4 2,160,652 - -1.6 2,160,652 - -1.1 2,160,702 + -0.2 2,160,708 + -1.2 2,160,708 + -0.7 2,160,708 + +0.7 2,160,708 + +2.0 2,160,709 - +0.5 2,160,709 - -0.2 2,160,765 - +0.8 2,160,779 + IAI47_10225 0.22 +1.8 2,160,779 + IAI47_10225 0.22 -0.8 2,160,779 + IAI47_10225 0.22 +1.8 2,160,780 - IAI47_10225 0.24 +0.0 2,160,780 - IAI47_10225 0.24 +2.9 2,160,780 - IAI47_10225 0.24 +1.2 2,160,780 - IAI47_10225 0.24 +1.0 2,160,780 - IAI47_10225 0.24 +4.1 2,160,807 + IAI47_10225 0.61 +0.2 2,160,807 + IAI47_10225 0.61 +0.6 2,160,808 - IAI47_10225 0.62 +1.3 2,160,808 - IAI47_10225 0.62 -0.4 2,160,808 - IAI47_10225 0.62 +0.8 2,160,808 - IAI47_10225 0.62 +2.3 2,160,808 - IAI47_10225 0.62 +0.7 2,160,839 + +1.2 2,160,839 + -0.4 2,160,839 + +0.6 2,160,839 + -1.4 2,160,839 + -1.7 2,160,840 - +1.8 2,160,840 - +0.3 2,160,840 - -0.4 2,160,860 - +1.5 2,161,048 + +0.6 2,161,048 + +0.2 2,161,048 + +0.4 2,161,049 - +1.6 2,161,049 - +1.7 2,161,164 + IAI47_10230 0.18 +0.8 2,161,165 - IAI47_10230 0.18 -0.0 2,161,203 + IAI47_10230 0.22 -0.1 2,161,227 - IAI47_10230 0.24 +1.5 2,161,259 + IAI47_10230 0.28 +1.8 2,161,680 - IAI47_10230 0.69 -1.1 2,161,680 - IAI47_10230 0.69 +1.5 2,161,680 - IAI47_10230 0.69 +0.4 2,161,680 - IAI47_10230 0.69 -0.8 2,161,682 + IAI47_10230 0.69 +0.1 2,161,682 + IAI47_10230 0.69 -0.6 2,161,682 + IAI47_10230 0.69 +1.0 2,161,682 + IAI47_10230 0.69 +1.1 2,161,683 - IAI47_10230 0.69 +1.8 2,161,683 - IAI47_10230 0.69 +1.4 2,161,817 + IAI47_10230 0.82 -1.0 2,161,818 - IAI47_10230 0.83 -0.9 2,161,818 - IAI47_10230 0.83 +1.8 2,161,818 - IAI47_10230 0.83 +0.9 2,161,818 - IAI47_10230 0.83 +1.5 2,161,819 + IAI47_10230 0.83 -0.5 2,161,819 + IAI47_10230 0.83 +1.1 2,161,820 - IAI47_10230 0.83 +1.0 2,161,820 - IAI47_10230 0.83 +2.3 2,161,820 - IAI47_10230 0.83 +1.1 2,161,820 - IAI47_10230 0.83 +1.8 2,161,820 - IAI47_10230 0.83 +1.0 2,161,820 - IAI47_10230 0.83 +0.3 2,161,820 - IAI47_10230 0.83 +1.5 2,161,820 - IAI47_10230 0.83 +0.4 2,161,820 - IAI47_10230 0.83 +1.2 2,161,820 - IAI47_10230 0.83 +1.2
Or see this region's nucleotide sequence