Experiment: Varel_Bryant medium with Zinc Pyrithione 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2141 and BT2142 are separated by 8 nucleotides BT2142 and BT2143 are separated by 351 nucleotides
BT2141: BT2141 - putative N-acetylmuramoyl-L-alanine amidase amiA (NCBI ptt file), at 2,694,941 to 2,696,203
BT2141
BT2142: BT2142 - conserved hypothetical protein, with a conserved domain (NCBI ptt file), at 2,696,212 to 2,697,105
BT2142
BT2143: BT2143 - chromosomal replication initiator protein dnaA (NCBI ptt file), at 2,697,457 to 2,698,869
BT2143
Position (kb)
2696
2697
2698 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2695.224 kb on - strand, within BT2141 at 2695.224 kb on - strand, within BT2141 at 2695.226 kb on - strand, within BT2141 at 2695.427 kb on + strand, within BT2141 at 2695.451 kb on + strand, within BT2141 at 2695.484 kb on + strand, within BT2141 at 2695.514 kb on + strand, within BT2141 at 2695.514 kb on + strand, within BT2141 at 2695.514 kb on + strand, within BT2141 at 2695.730 kb on + strand, within BT2141 at 2695.749 kb on - strand, within BT2141 at 2695.858 kb on + strand, within BT2141 at 2695.859 kb on - strand, within BT2141 at 2695.901 kb on - strand, within BT2141 at 2695.901 kb on - strand, within BT2141 at 2695.943 kb on - strand, within BT2141 at 2696.022 kb on - strand, within BT2141 at 2696.022 kb on - strand, within BT2141 at 2696.045 kb on + strand, within BT2141 at 2696.047 kb on + strand, within BT2141 at 2696.069 kb on + strand, within BT2141 at 2696.082 kb on + strand at 2696.197 kb on + strand at 2696.308 kb on - strand, within BT2142 at 2696.433 kb on - strand, within BT2142 at 2696.703 kb on - strand, within BT2142 at 2696.996 kb on - strand, within BT2142 at 2697.132 kb on + strand at 2697.161 kb on + strand at 2697.184 kb on + strand at 2697.394 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Zinc Pyrithione 0.0125 mM remove 2,695,224 - BT2141 0.22 +1.0 2,695,224 - BT2141 0.22 -1.8 2,695,226 - BT2141 0.23 +1.0 2,695,427 + BT2141 0.38 -0.4 2,695,451 + BT2141 0.40 -0.5 2,695,484 + BT2141 0.43 -0.0 2,695,514 + BT2141 0.45 -2.5 2,695,514 + BT2141 0.45 +0.5 2,695,514 + BT2141 0.45 +2.3 2,695,730 + BT2141 0.62 -1.6 2,695,749 - BT2141 0.64 -0.0 2,695,858 + BT2141 0.73 -1.4 2,695,859 - BT2141 0.73 -0.4 2,695,901 - BT2141 0.76 -0.0 2,695,901 - BT2141 0.76 -0.6 2,695,943 - BT2141 0.79 -0.6 2,696,022 - BT2141 0.86 +1.0 2,696,022 - BT2141 0.86 +1.0 2,696,045 + BT2141 0.87 +0.1 2,696,047 + BT2141 0.88 -1.4 2,696,069 + BT2141 0.89 -0.6 2,696,082 + -0.6 2,696,197 + +1.6 2,696,308 - BT2142 0.11 +1.6 2,696,433 - BT2142 0.25 -0.6 2,696,703 - BT2142 0.55 -2.0 2,696,996 - BT2142 0.88 -1.3 2,697,132 + +1.0 2,697,161 + -0.7 2,697,184 + +0.4 2,697,394 + -0.0
Or see this region's nucleotide sequence