Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_16145 and IAI47_16150 are separated by 29 nucleotides IAI47_16150 and IAI47_16155 are separated by 306 nucleotides IAI47_16155 and IAI47_16160 are separated by 36 nucleotides
IAI47_16145: IAI47_16145 - isoleucine--tRNA ligase, at 3,416,964 to 3,419,780
_16145
IAI47_16150: IAI47_16150 - bifunctional riboflavin kinase/FAD synthetase, at 3,419,810 to 3,420,739
_16150
IAI47_16155: IAI47_16155 - 30S ribosomal protein S20, at 3,421,046 to 3,421,309
_16155
IAI47_16160: IAI47_16160 - transcriptional activator NhaR, at 3,421,346 to 3,422,263
_16160
Position (kb)
3419
3420
3421 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3420.772 kb on - strand at 3420.772 kb on - strand at 3420.772 kb on - strand at 3420.772 kb on - strand at 3420.774 kb on - strand at 3420.774 kb on - strand at 3420.774 kb on - strand at 3420.796 kb on - strand at 3420.823 kb on - strand at 3420.856 kb on + strand at 3420.857 kb on - strand at 3420.857 kb on - strand at 3420.857 kb on - strand at 3420.871 kb on + strand at 3420.876 kb on - strand at 3420.880 kb on + strand at 3420.880 kb on + strand at 3420.921 kb on + strand at 3420.955 kb on - strand at 3421.369 kb on + strand at 3421.370 kb on - strand at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.456 kb on + strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.457 kb on - strand, within IAI47_16160 at 3421.461 kb on + strand, within IAI47_16160 at 3421.461 kb on + strand, within IAI47_16160 at 3421.461 kb on + strand, within IAI47_16160 at 3421.461 kb on + strand, within IAI47_16160 at 3421.461 kb on + strand, within IAI47_16160 at 3421.462 kb on - strand, within IAI47_16160 at 3421.462 kb on - strand, within IAI47_16160 at 3421.462 kb on - strand, within IAI47_16160 at 3421.462 kb on - strand, within IAI47_16160 at 3421.477 kb on + strand, within IAI47_16160 at 3421.478 kb on - strand, within IAI47_16160 at 3421.478 kb on - strand, within IAI47_16160 at 3421.550 kb on + strand, within IAI47_16160 at 3421.550 kb on + strand, within IAI47_16160 at 3421.550 kb on + strand, within IAI47_16160 at 3421.550 kb on + strand, within IAI47_16160 at 3421.550 kb on + strand, within IAI47_16160 at 3421.551 kb on - strand, within IAI47_16160 at 3421.561 kb on + strand, within IAI47_16160 at 3421.562 kb on - strand, within IAI47_16160 at 3421.562 kb on - strand, within IAI47_16160 at 3421.562 kb on - strand, within IAI47_16160 at 3421.636 kb on + strand, within IAI47_16160 at 3421.637 kb on - strand, within IAI47_16160
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 3,420,772 - +2.9 3,420,772 - +0.2 3,420,772 - +1.5 3,420,772 - +0.3 3,420,774 - +1.3 3,420,774 - +0.7 3,420,774 - +1.2 3,420,796 - -0.2 3,420,823 - +0.7 3,420,856 + -0.4 3,420,857 - +1.3 3,420,857 - +0.1 3,420,857 - +2.0 3,420,871 + +0.0 3,420,876 - -0.5 3,420,880 + -0.3 3,420,880 + +0.5 3,420,921 + -0.3 3,420,955 - -2.1 3,421,369 + -1.5 3,421,370 - -1.4 3,421,456 + IAI47_16160 0.12 -3.1 3,421,456 + IAI47_16160 0.12 -0.8 3,421,456 + IAI47_16160 0.12 -2.2 3,421,456 + IAI47_16160 0.12 -0.5 3,421,456 + IAI47_16160 0.12 -1.4 3,421,456 + IAI47_16160 0.12 -0.2 3,421,456 + IAI47_16160 0.12 +0.6 3,421,456 + IAI47_16160 0.12 -2.2 3,421,456 + IAI47_16160 0.12 +0.3 3,421,456 + IAI47_16160 0.12 -1.4 3,421,456 + IAI47_16160 0.12 +1.4 3,421,456 + IAI47_16160 0.12 +0.7 3,421,457 - IAI47_16160 0.12 -0.3 3,421,457 - IAI47_16160 0.12 -0.8 3,421,457 - IAI47_16160 0.12 -1.3 3,421,457 - IAI47_16160 0.12 +1.6 3,421,457 - IAI47_16160 0.12 -0.9 3,421,457 - IAI47_16160 0.12 -0.1 3,421,457 - IAI47_16160 0.12 -2.0 3,421,457 - IAI47_16160 0.12 -1.7 3,421,461 + IAI47_16160 0.13 -0.4 3,421,461 + IAI47_16160 0.13 +0.1 3,421,461 + IAI47_16160 0.13 -1.2 3,421,461 + IAI47_16160 0.13 -0.1 3,421,461 + IAI47_16160 0.13 +0.0 3,421,462 - IAI47_16160 0.13 -1.8 3,421,462 - IAI47_16160 0.13 -0.7 3,421,462 - IAI47_16160 0.13 -0.0 3,421,462 - IAI47_16160 0.13 -0.1 3,421,477 + IAI47_16160 0.14 -0.5 3,421,478 - IAI47_16160 0.14 -0.3 3,421,478 - IAI47_16160 0.14 -0.1 3,421,550 + IAI47_16160 0.22 -0.3 3,421,550 + IAI47_16160 0.22 +0.7 3,421,550 + IAI47_16160 0.22 +0.6 3,421,550 + IAI47_16160 0.22 +0.4 3,421,550 + IAI47_16160 0.22 -2.0 3,421,551 - IAI47_16160 0.22 -0.6 3,421,561 + IAI47_16160 0.23 -1.6 3,421,562 - IAI47_16160 0.24 -0.6 3,421,562 - IAI47_16160 0.24 -1.7 3,421,562 - IAI47_16160 0.24 +1.4 3,421,636 + IAI47_16160 0.32 +2.0 3,421,637 - IAI47_16160 0.32 -1.0
Or see this region's nucleotide sequence